PMID- 36006542
OWN - NLM
STAT- MEDLINE
DCOM- 20221013
LR  - 20221018
IS  - 1573-742X (Electronic)
IS  - 0929-5305 (Linking)
VI  - 54
IP  - 3
DP  - 2022 Oct
TI  - MicroRNA-342-3p loaded by human umbilical cord mesenchymal stem cells-derived 
      exosomes attenuates deep vein thrombosis by downregulating EDNRA.
PG  - 411-419
LID - 10.1007/s11239-022-02694-6 [doi]
AB  - Exosomes (exos) exert biological functions to maintain the dynamic balance of 
      cells and tissues by transferring biological cargo to recipient cells. Thus, this 
      study explored human umbilical cord mesenchymal stem cells (hucMSCs)-derived exo 
      transfer of microRNA (miR)-342-3p in deep vein thrombosis (DVT). DVT rat models 
      were established via inferior vena cava (IVC) ligation. HucMSCs-exos were 
      extracted and injected into rats with DVT to observe whether they could 
      influences thrombus formation in vivo. HucMSCs-exos were co-cultured with human 
      umbilical vein endothelial cells (HUVECs) in vitro to observe angiogenesis. 
      miR-342-3p and endothelin A receptor (EDNRA) expression in rats with DVT, as well 
      as their interaction was analyzed. miR-342-3p was downregulated and EDNRA was 
      upregulated in rats with DVT. HucMSCs-exos inhibited the formation of thrombus in 
      rats with DVT, as well as promoted angiogenesis of HUVECs. Upregulated miR-342-3p 
      delivery by hucMSCs-exos alleviated DVT in rats and improved angiogenesis of 
      HUVECs. miR-342-3p targeted EDNRA, and the effect of hucMSCs-exos transfer of 
      upregulated miR-342-3p was rescued by overexpressing EDNRA. Briefly, miR-342-3p 
      loaded by hucMSCs-exos attenuates DVT by downregulating EDNRA, offering a novel 
      direction to treat DVT.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Pan, Zhiyu
AU  - Pan Z
AD  - Department of Vascular Surgery, Minhang Hospital, Fudan University, No. 39 
      Xinling Road, Minhang District, Shanghai, 201100, China.
FAU - Chen, Qian
AU  - Chen Q
AD  - Department of Vascular Surgery, Minhang Hospital, Fudan University, No. 39 
      Xinling Road, Minhang District, Shanghai, 201100, China.
FAU - Ding, Hongjian
AU  - Ding H
AD  - Department of Vascular Surgery, Minhang Hospital, Fudan University, No. 39 
      Xinling Road, Minhang District, Shanghai, 201100, China.
FAU - Li, Huaqing
AU  - Li H
AUID- ORCID: 0000-0001-9568-9135
AD  - Department of Vascular Surgery, Minhang Hospital, Fudan University, No. 39 
      Xinling Road, Minhang District, Shanghai, 201100, China. Lihuaqing166@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220825
PL  - Netherlands
TA  - J Thromb Thrombolysis
JT  - Journal of thrombosis and thrombolysis
JID - 9502018
RN  - 0 (MIRN342 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptor, Endothelin A)
SB  - IM
MH  - Animals
MH  - *Exosomes/genetics
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Rats
MH  - Receptor, Endothelin A/metabolism
MH  - Umbilical Cord/metabolism
MH  - *Venous Thrombosis/genetics/metabolism/therapy
OTO - NOTNLM
OT  - Angiogenesis
OT  - Deep vein thrombosis
OT  - Endothelin A receptor
OT  - Exosomes
OT  - Human umbilical cord mesenchymal stem cells
OT  - microRNA-342-3p
EDAT- 2022/08/26 06:00
MHDA- 2022/10/14 06:00
CRDT- 2022/08/25 11:22
PHST- 2022/08/06 00:00 [accepted]
PHST- 2022/08/26 06:00 [pubmed]
PHST- 2022/10/14 06:00 [medline]
PHST- 2022/08/25 11:22 [entrez]
AID - 10.1007/s11239-022-02694-6 [pii]
AID - 10.1007/s11239-022-02694-6 [doi]
PST - ppublish
SO  - J Thromb Thrombolysis. 2022 Oct;54(3):411-419. doi: 10.1007/s11239-022-02694-6. 
      Epub 2022 Aug 25.