PMID- 36010314
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 12
IP  - 8
DP  - 2022 Aug 14
TI  - Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 
      2018 to 2019.
LID - 10.3390/diagnostics12081964 [doi]
LID - 1964
AB  - OBJECTIVE: To investigate the common pathogens of viral encephalitis (VE) in 
      children, and to provide guidance for the empirical diagnosis and treatment of 
      patients with VE. METHODS: A total of 227 cerebrospinal fluid (CSF) samples were 
      collected from pediatric patients with VE in Zhejiang province from January 2018 
      to December 2019. The samples were tested using multiplex and singleplex Reverse 
      Transcription-Polymerase Chain Reaction (RT-PCR) with primers specific to 
      enterovirus (EV), varicella-zoster virus (VZV), mumps virus (MuV), 
      cytomegalovirus (CMV), herpes simplex virus type 1 (HSV-1)/type 2 (HSV-2), 
      Epstein-Barr virus (EBV), and human herpesvirus 6 (HHV-6). The data of the two 
      analyses were compared and then verified using Sanger sequencing. RESULTS: Of the 
      227 CSF samples, 90 were shown to be positive for multiplex RT-PCR with a 
      positivity rate of 39.65% and a 95% confidence interval (33.2%, 46.1%). EV was 
      the most common cause of VE, followed by EBV, HHV-6, MuV, CMV, VZV, and HSV-1. 
      Most included cases occurred in summer, accounting for 49.78% of all cases. For 
      EV, EBV, and HSV-2, multiplex RT-PCR showed a positivity rate of 34.36%, which 
      was not statistically different from that of 30.4% shown by singleplex RT-PCR. 
      The sequences of EV, EBV, VZV, MuV, CMV, HSV-1, HHV-6, and HSV-2 were confirmed 
      by sequencing the PCR products obtained from multiplex and singleplex PCR. 
      CONCLUSIONS: In children, VE is more prevalent in the summer than in other 
      seasons in Zhejiang province, and EV may be the most common causative pathogen.
FAU - Liu, Juan-Juan
AU  - Liu JJ
AD  - Department of Infectious Diseases, National Clinical Research Center for Child 
      Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 
      310052, China.
FAU - Teng, Li-Ping
AU  - Teng LP
AD  - Department of Infectious Diseases, National Clinical Research Center for Child 
      Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 
      310052, China.
AD  - Department of Rheumatology Immunology and Allergy, National Clinical Research 
      Center for Child Health, Children's Hospital, Zhejiang University School of 
      Medicine, Hangzhou 310052, China.
FAU - Hua, Chun-Zhen
AU  - Hua CZ
AUID- ORCID: 0000-0001-9311-6229
AD  - Department of Infectious Diseases, National Clinical Research Center for Child 
      Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 
      310052, China.
FAU - Xie, Yong-Ping
AU  - Xie YP
AD  - Department of Infectious Diseases, National Clinical Research Center for Child 
      Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 
      310052, China.
FAU - Pan, Yan-Xiang
AU  - Pan YX
AD  - Department of Clinical Laboratory Center, National Clinical Research Center for 
      Child Health, Children's Hospital, Zhejiang University School of Medicine, 
      Hangzhou 310052, China.
FAU - Hu, Bo-Fei
AU  - Hu BF
AD  - Department of Infectious Diseases, National Clinical Research Center for Child 
      Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 
      310052, China.
FAU - Hu, Wei-Lin
AU  - Hu WL
AD  - Department of Infectious Diseases, National Clinical Research Center for Child 
      Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 
      310052, China.
AD  - Department of Medical Microbiology, Zhejiang University School of Medicine, 
      Hangzhou 310058, China.
FAU - Wang, Wei-Jian
AU  - Wang WJ
AD  - Department of Research and Development, Health Gene Technologies Co., Ltd., 
      Ningbo 315000, China.
LA  - eng
PT  - Journal Article
DEP - 20220814
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC9407060
OTO - NOTNLM
OT  - children
OT  - etiology
OT  - multiplex RT-PCR
OT  - viral encephalitis
COIS- The authors declare no conflict of interest.
EDAT- 2022/08/27 06:00
MHDA- 2022/08/27 06:01
PMCR- 2022/08/14
CRDT- 2022/08/26 01:10
PHST- 2022/05/30 00:00 [received]
PHST- 2022/08/08 00:00 [revised]
PHST- 2022/08/11 00:00 [accepted]
PHST- 2022/08/26 01:10 [entrez]
PHST- 2022/08/27 06:00 [pubmed]
PHST- 2022/08/27 06:01 [medline]
PHST- 2022/08/14 00:00 [pmc-release]
AID - diagnostics12081964 [pii]
AID - diagnostics-12-01964 [pii]
AID - 10.3390/diagnostics12081964 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2022 Aug 14;12(8):1964. doi: 10.3390/diagnostics12081964.