PMID- 36012318
OWN - NLM
STAT- MEDLINE
DCOM- 20220829
LR  - 20220830
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 16
DP  - 2022 Aug 12
TI  - Welding Fume Instillation in Isolated Perfused Mouse Lungs-Effects of Zinc- and 
      Copper-Containing Welding Fumes.
LID - 10.3390/ijms23169052 [doi]
LID - 9052
AB  - Zinc- and copper-containing welding fumes can cause systemic inflammation after 
      exposure in humans. Recent ex vivo studies have shown that the observed 
      inflammation originates from exposed immune cells. In vitro studies identified 
      the soluble fraction of metal particles as the main effectors. Isolated perfused 
      mouse lungs (IPLs) were perfused and ventilated for 270 min. Lungs were instilled 
      with saline solution (control), welding fume particle suspension (WFs) or the 
      soluble fraction of the welding fumes (SF-WFs). Bronchoalveolar lavage fluid 
      (BALF) and perfusate samples were analyzed for cytokine levels and lung tissue 
      mRNA expression levels were analyzed via RT-PCR. All lungs instilled with WFs did 
      not complete the experiments due to a fatal reduction in tidal volume. 
      Accordingly, IL-6 and MPO levels were significantly higher in BALF of WF lungs 
      compared to the control. IL-6 and MPO mRNA expression levels were also increased 
      for WFs. Lungs instilled with SF-WFs only showed mild reactions in tidal volume, 
      with BALF and mRNA expression levels not significantly differing from the 
      control. Zinc- and copper-containing welding fume particles adversely affect IPLs 
      when instilled, as evidenced by the fatal loss in tidal volume and increased 
      cytokine expression and secretion. The effects are mainly caused by the 
      particles, not by the soluble fraction.
FAU - Krabbe, Julia
AU  - Krabbe J
AUID- ORCID: 0000-0003-4205-9783
AD  - Institute of Occupational, Social and Environmental Medicine, Medical Faculty, 
      RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
FAU - Kraus, Thomas
AU  - Kraus T
AD  - Institute of Occupational, Social and Environmental Medicine, Medical Faculty, 
      RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
FAU - Krabbe, Hanif
AU  - Krabbe H
AD  - European Vascular Centre Aachen-Maastricht, Department of Vascular Surgery, 
      Medical Faculty, University Hospital RWTH Aachen, Pauwelsstrasse 30, 52074 Aachen, 
      Germany.
FAU - Martin, Christian
AU  - Martin C
AD  - Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen 
      University, Wendlingweg 2, 52074 Aachen, Germany.
FAU - Ziegler, Patrick
AU  - Ziegler P
AD  - Institute of Occupational, Social and Environmental Medicine, Medical Faculty, 
      RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20220812
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Air Pollutants, Occupational)
RN  - 0 (Cytokines)
RN  - 0 (Gases)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 789U1901C5 (Copper)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - *Air Pollutants, Occupational/toxicity
MH  - Animals
MH  - Copper/pharmacology
MH  - Cytokines/metabolism
MH  - Gases/pharmacology
MH  - Inflammation/etiology
MH  - Inhalation Exposure
MH  - Interleukin-6/genetics/pharmacology
MH  - Lung/metabolism
MH  - Mice
MH  - RNA, Messenger/genetics
MH  - *Welding
MH  - Zinc/pharmacology
PMC - PMC9408907
OTO - NOTNLM
OT  - copper
OT  - isolated perfused lung
OT  - myeloperoxidase
OT  - welding fumes
OT  - zinc
COIS- All authors have carried out research in the past funded by the Institution for 
      Statutory Accident Insurance and Prevention in the woodworking and metalworking 
      industry (BGHM), Mainz, Germany, with unrestricted grants to the University 
      Hospital RWTH Aachen. J.K. and T.K. have held paid lectures about welding fume 
      effects.
EDAT- 2022/08/27 06:00
MHDA- 2022/08/30 06:00
PMCR- 2022/08/12
CRDT- 2022/08/26 01:22
PHST- 2022/06/29 00:00 [received]
PHST- 2022/08/08 00:00 [revised]
PHST- 2022/08/09 00:00 [accepted]
PHST- 2022/08/26 01:22 [entrez]
PHST- 2022/08/27 06:00 [pubmed]
PHST- 2022/08/30 06:00 [medline]
PHST- 2022/08/12 00:00 [pmc-release]
AID - ijms23169052 [pii]
AID - ijms-23-09052 [pii]
AID - 10.3390/ijms23169052 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Aug 12;23(16):9052. doi: 10.3390/ijms23169052.