PMID- 36012702
OWN - NLM
STAT- MEDLINE
DCOM- 20220829
LR  - 20230308
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 16
DP  - 2022 Aug 21
TI  - Efficient Anchoring of Erianthus arundinaceus Chromatin Introgressed into 
      Sugarcane by Specific Molecular Markers.
LID - 10.3390/ijms23169435 [doi]
LID - 9435
AB  - Erianthus arundinaceus is a valuable gene reservoir for sugarcane improvement. 
      However, insufficient molecular markers for high-accuracy identification and 
      tracking of the introgression status of E. arundinaceus chromatin impede 
      sugarcane breeding. Fortunately, suppression subtractive hybridization (SSH) 
      technology provides an excellent opportunity for the development of 
      high-throughput E. arundinaceus-specific molecular markers at a reasonable cost. 
      In this study, we constructed a SSH library of E. arundinaceus. In total, 288 
      clones of E. arundinaceus-specific repetitive sequences were screened out and 
      their distribution patterns on chromosomes were characterized by fluorescence in 
      situ hybridization (FISH). A subtelomeric repetitive sequence Ea086 and a 
      diffusive repetitive sequence Ea009, plus 45S rDNA-bearing E. arundinaceus 
      chromosome repetitive sequence EaITS were developed as E. arundinaceus-specific 
      molecular markers, namely, Ea086-128, Ea009-257, and EaITS-278, covering all the 
      E. arundinaceus chromosomes for high-accuracy identification of putative progeny. 
      Both Ea086-128 and Ea009-257 were successfully applied to identify the 
      authenticity of F1, BC1, BC2, BC3, and BC4 progeny between sugarcane and E. 
      arundinaceus. In addition, EaITS-278 was a 45S rDNA-bearing E. arundinaceus 
      chromosome-specific molecular marker for rapid tracking of the inherited status 
      of this chromosome in a sugarcane background. Three BC3 progeny had apparently 
      lost the 45S rDNA-bearing E. arundinaceus chromosome. We reported herein a highly 
      effective and reliable SSH-based technology for discovery of high-throughput E. 
      arundinaceus-specific sequences bearing high potential as molecular markers. 
      Given its reliability and savings in time and efforts, the method is also 
      suitable for development of species-specific molecular markers for other 
      important wild relatives to accelerate introgression of wild relatives into 
      sugarcane.
FAU - Wu, Jiayun
AU  - Wu J
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - Guangdong Sugarcane Genetic Improvement Engineering Center, Institute of Nanfan 
      and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, China.
FAU - Zhang, Mingxiao
AU  - Zhang M
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
FAU - Liu, Jiarui
AU  - Liu J
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
FAU - Huang, Yongji
AU  - Huang Y
AUID- ORCID: 0000-0003-2513-5983
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
FAU - Xu, Liangnian
AU  - Xu L
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
FAU - Deng, Zuhu
AU  - Deng Z
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - State Key Laboratory for Protection and Utilization of Subtropical 
      Agro-Bioresources, Guangxi University, Nanning 530004, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
FAU - Zhao, Xinwang
AU  - Zhao X
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou 350002, China.
AD  - State Key Laboratory for Protection and Utilization of Subtropical 
      Agro-Bioresources, Guangxi University, Nanning 530004, China.
AD  - Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, 
      Fujian Agriculture and Forestry University, Fuzhou 350002, China.
LA  - eng
GR  - 32001605/National Natural Science Foundation of China/
GR  - KFA17626A, KFA20082A, KHF210001A/Special Fund for Science and Technology 
      Innovation of Fujian Agriculture and Forestry University/
PT  - Journal Article
DEP - 20220821
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Chromatin)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromatin/genetics
MH  - Chromosomes, Plant/genetics
MH  - DNA, Ribosomal
MH  - Genetic Markers
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Breeding
MH  - Poaceae/genetics
MH  - Reproducibility of Results
MH  - *Saccharum/genetics
PMC - PMC9408830
OTO - NOTNLM
OT  - Erianthus arundinaceus
OT  - chromosome
OT  - fluorescence in situ hybridization (FISH)
OT  - species-specific molecular marker
OT  - sugarcane
OT  - suppression subtractive hybridization (SSH)
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/27 06:00
MHDA- 2022/08/30 06:00
PMCR- 2022/08/21
CRDT- 2022/08/26 01:25
PHST- 2022/08/08 00:00 [received]
PHST- 2022/08/18 00:00 [revised]
PHST- 2022/08/18 00:00 [accepted]
PHST- 2022/08/26 01:25 [entrez]
PHST- 2022/08/27 06:00 [pubmed]
PHST- 2022/08/30 06:00 [medline]
PHST- 2022/08/21 00:00 [pmc-release]
AID - ijms23169435 [pii]
AID - ijms-23-09435 [pii]
AID - 10.3390/ijms23169435 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Aug 21;23(16):9435. doi: 10.3390/ijms23169435.