PMID- 36015366
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 14
IP  - 8
DP  - 2022 Aug 20
TI  - Association of Indocyanine Green with Chitosan Oleate Coated PLGA Nanoparticles 
      for Photodynamic Therapy.
LID - 10.3390/pharmaceutics14081740 [doi]
LID - 1740
AB  - Indocyanine green (ICG) is a safe dye widely used in the biomedical field. Its 
      photodynamic effect (PDT), originating from laser irradiation at 803 nm, opens 
      interesting perspectives in theranostic applications. To overcome its low water 
      stability, ICG can be shielded with nanoparticles (NPs). In this work, previously 
      developed NPs based on poly lactic-co-glycolic acid (PLGA) coated with chitosan 
      oleate (CS-OA) and loaded with resveratrol as a hydrophobic model drug have been 
      proposed as an ICG carrier. These systems have been selected for their observed 
      immunostimulatory properties. The possible loading of the dye by adsorption onto 
      NP surface by electrostatic interaction was studied here in comparison with the 
      encapsulation into the PLGA core. The ICG-chitosan (CS) interaction has been 
      characterized by spectrophotometry, spectroscopy and in-cell in vitro assays. 
      Fluorescence quenching was observed due to the ionic interaction between ICG and 
      CS and was studied considering the dye:polymer stoichiometry and the effect of 
      the NP dilution in cell culture medium (DMEM). The NP systems have been compared 
      in vitro, assessing their behaviour in Caco-2 cell lines. A reduction in cell 
      viability was observed after irradiation of ICG associated with NPs, evident also 
      for the samples loaded by adsorption. These findings open the opportunity to 
      exploit the association of PDT's effect on ICG with the properties of CS-OA 
      coated NPs, whose immunostimulatory effect can be associated with PDT mechanism 
      in cancer therapy.
FAU - Miele, Dalila
AU  - Miele D
AUID- ORCID: 0000-0001-6396-7452
AD  - Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy.
FAU - Sorrenti, Milena
AU  - Sorrenti M
AUID- ORCID: 0000-0001-8249-5761
AD  - Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy.
FAU - Catenacci, Laura
AU  - Catenacci L
AUID- ORCID: 0000-0001-6791-3785
AD  - Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy.
FAU - Minzioni, Paolo
AU  - Minzioni P
AUID- ORCID: 0000-0002-3087-8602
AD  - Department of Electrical, Computer and Biomedical Engineering, University of 
      Pavia, 27100 Pavia, Italy.
FAU - Marrubini, Giorgio
AU  - Marrubini G
AUID- ORCID: 0000-0003-1399-5783
AD  - Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy.
FAU - Amendola, Valeria
AU  - Amendola V
AUID- ORCID: 0000-0001-5219-6074
AD  - Department of Chemistry, University of Pavia, 27100 Pavia, Italy.
FAU - Maestri, Marcello
AU  - Maestri M
AUID- ORCID: 0000-0002-5693-9151
AD  - IRCCS Policlinico San Matteo Foundation, 27100 Pavia, Italy.
FAU - Giunchedi, Paolo
AU  - Giunchedi P
AUID- ORCID: 0000-0002-2471-1355
AD  - Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100 
      Sassari, Italy.
FAU - Bonferoni, Maria Cristina
AU  - Bonferoni MC
AUID- ORCID: 0000-0002-1194-9372
AD  - Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy.
LA  - eng
PT  - Journal Article
DEP - 20220820
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC9414095
OTO - NOTNLM
OT  - PLGA nanoparticles
OT  - chitosan oleate
OT  - indocyanine green
OT  - photodynamic effect
COIS- The authors declare no conflict of interest.
EDAT- 2022/08/27 06:00
MHDA- 2022/08/27 06:01
PMCR- 2022/08/20
CRDT- 2022/08/26 01:42
PHST- 2022/07/31 00:00 [received]
PHST- 2022/08/14 00:00 [revised]
PHST- 2022/08/18 00:00 [accepted]
PHST- 2022/08/26 01:42 [entrez]
PHST- 2022/08/27 06:00 [pubmed]
PHST- 2022/08/27 06:01 [medline]
PHST- 2022/08/20 00:00 [pmc-release]
AID - pharmaceutics14081740 [pii]
AID - pharmaceutics-14-01740 [pii]
AID - 10.3390/pharmaceutics14081740 [doi]
PST - epublish
SO  - Pharmaceutics. 2022 Aug 20;14(8):1740. doi: 10.3390/pharmaceutics14081740.