PMID- 36016406
OWN - NLM
STAT- MEDLINE
DCOM- 20220829
LR  - 20230310
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 14
IP  - 8
DP  - 2022 Aug 15
TI  - The Genetic Stability, Replication Kinetics and Cytopathogenicity of Recombinant 
      Avian Coronaviruses with a T16A or an A26F Mutation within the E Protein Is 
      Cell-Type Dependent.
LID - 10.3390/v14081784 [doi]
LID - 1784
AB  - The envelope (E) protein of the avian coronavirus infectious bronchitis virus 
      (IBV) is a small-membrane protein present in two forms during infection: a 
      monomer and a pentameric ion channel. Each form has an independent role during 
      replication; the monomer disrupts the secretory pathway, and the pentamer 
      facilitates virion production. The presence of a T16A or A26F mutation within E 
      exclusively generates the pentameric or monomeric form, respectively. We 
      generated two recombinant IBVs (rIBVs) based on the apathogenic molecular clone 
      Beau-R, containing either a T16A or A26F mutation, denoted as BeauR-T16A and 
      BeauR-A26F. The replication and genetic stability of the rIBVs were assessed in 
      several different cell types, including primary and continuous cells, ex vivo 
      tracheal organ cultures (TOCs) and in ovo. Different replication profiles were 
      observed between cell cultures of different origins. BeauR-A26F replicated to a 
      lower level than Beau-R in Vero cells and in ovo but not in DF1, primary chicken 
      kidney (CK) cells or TOCs. Genetic stability and cytopathic effects were found to 
      differ depending on the cell system. The effect of the T16A and A26F mutations 
      appear to be cell-type dependent, which, therefore, highlights the importance of 
      cell type in the investigation of the IBV E protein.
FAU - Webb, Isobel
AU  - Webb I
AD  - The Pirbright Institute, Woking GU24 0NF, UK.
AD  - School of Cellular and Molecular Medicine, Faculty of Life Sciences, The 
      University of Bristol, Bristol BS8 1TH, UK.
FAU - Keep, Sarah
AU  - Keep S
AUID- ORCID: 0000-0001-9583-630X
AD  - The Pirbright Institute, Woking GU24 0NF, UK.
FAU - Littolff, Kieran
AU  - Littolff K
AD  - The Pirbright Institute, Woking GU24 0NF, UK.
FAU - Stuart, Jamie
AU  - Stuart J
AD  - The Pirbright Institute, Woking GU24 0NF, UK.
FAU - Freimanis, Graham
AU  - Freimanis G
AUID- ORCID: 0000-0001-8186-2638
AD  - The Pirbright Institute, Woking GU24 0NF, UK.
FAU - Britton, Paul
AU  - Britton P
AD  - The Pirbright Institute, Woking GU24 0NF, UK.
FAU - Davidson, Andrew D
AU  - Davidson AD
AD  - School of Cellular and Molecular Medicine, Faculty of Life Sciences, The 
      University of Bristol, Bristol BS8 1TH, UK.
FAU - Maier, Helena J
AU  - Maier HJ
AUID- ORCID: 0000-0002-2901-5578
AD  - The Pirbright Institute, Woking GU24 0NF, UK.
FAU - Bickerton, Erica
AU  - Bickerton E
AUID- ORCID: 0000-0002-4012-1283
AD  - The Pirbright Institute, Woking GU24 0NF, UK.
LA  - eng
GR  - BBS/E/I/00007035/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/00007031/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/00007039/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/00007034/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/00007030/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/00007037/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/COV07001/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
GR  - BBS/E/I/00007038/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220815
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Chlorocebus aethiops
MH  - *Coronavirus Infections/veterinary
MH  - *Gammacoronavirus
MH  - *Infectious bronchitis virus/genetics
MH  - Mutation
MH  - Vero Cells
PMC - PMC9415719
OTO - NOTNLM
OT  - avian coronavirus
OT  - cell type
OT  - chicken
OT  - envelope protein
OT  - viroporin
COIS- The authors declare no conflict of interest.
EDAT- 2022/08/27 06:00
MHDA- 2022/08/30 06:00
PMCR- 2022/08/15
CRDT- 2022/08/26 01:49
PHST- 2022/07/20 00:00 [received]
PHST- 2022/08/09 00:00 [revised]
PHST- 2022/08/11 00:00 [accepted]
PHST- 2022/08/26 01:49 [entrez]
PHST- 2022/08/27 06:00 [pubmed]
PHST- 2022/08/30 06:00 [medline]
PHST- 2022/08/15 00:00 [pmc-release]
AID - v14081784 [pii]
AID - viruses-14-01784 [pii]
AID - 10.3390/v14081784 [doi]
PST - epublish
SO  - Viruses. 2022 Aug 15;14(8):1784. doi: 10.3390/v14081784.