PMID- 36017065 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220827 IS - 1939-4551 (Print) IS - 1939-4551 (Electronic) IS - 1939-4551 (Linking) VI - 15 IP - 8 DP - 2022 Aug TI - Microbiome profiling of nasal extracellular vesicles in patients with allergic rhinitis. PG - 100674 LID - 10.1016/j.waojou.2022.100674 [doi] LID - 100674 AB - BACKGROUND: Nasal microbiota is crucial for the pathogenesis of allergic rhinitis (AR), which has been reported to be different from that of healthy individuals. However, no study has investigated the microbiota in nasal extracellular vesicles (EVs). We aimed to compare the microbiome composition and diversity in EVs between AR patients and healthy controls (HCs) and reveal the potential metabolic mechanisms in AR. METHODS: Eosinophil counts and serum immunoglobulin E (IgE) levels were measured in patients with AR (n = 20) and HCs (n = 19). Nasal EVs were identified using transmission electron microscopy and flow cytometry. 16S rRNA sequencing was used to profile the microbial communities. Alpha and beta diversities were analyzed to determine microbial diversity. Taxonomic abundance was analyzed based on the linear discriminant analysis effect size (LEfSe). Microbial metabolic pathways were characterized using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUst2) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. RESULTS: Eosinophils, total serum IgE, and IgE specific to Dermatophagoides were increased in patients with AR. Alpha diversity in nasal EVs from patients with AR was lower than that in HCs. Beta diversity showed microbiome differences between the AR and HCs groups. The microbial abundance was distinct between AR and HCs at different taxonomic levels. Significantly higher levels of the genera Acetobacter, Mycoplasma, Escherichia, and Halomonas were observed in AR patients than in HCs. Conversely, Zoogloea, Streptococcus, Burkholderia, and Pseudomonas were more abundant in the HCs group than in the AR group. Moreover, 35 microbial metabolic pathways recognized in AR patients and HCs, and 25 pathways were more abundant in the AR group. CONCLUSION: Patients with AR had distinct microbiota characteristics in nasal EVs compared to that in HCs. The metabolic mechanisms of the microbiota that regulate AR development were also different. These findings show that nasal fluid may reflect the specific pattern of microbiome EVs in patients with AR. CI - (c) 2022 The Author(s). FAU - Chiang, Tsai-Yeh AU - Chiang TY AD - Department of Otorhinolaryngology, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Xiamen Chang Gung Allergology Consortium, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. FAU - Yang, Yu-Ru AU - Yang YR AD - Department of Otorhinolaryngology, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. FAU - Zhuo, Ming-Ying AU - Zhuo MY AD - Department of Otorhinolaryngology, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. FAU - Yang, Feng AU - Yang F AD - Department of Otorhinolaryngology, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. FAU - Zhang, Ying-Fei AU - Zhang YF AD - Department of Otorhinolaryngology, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. FAU - Fu, Chia-Hsiang AU - Fu CH AD - Department of Otolaryngology-Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan City, Taiwan. AD - Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan City, Taiwan. FAU - Lee, Ta-Jen AU - Lee TJ AD - Department of Otorhinolaryngology, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Department of Otolaryngology-Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan City, Taiwan. AD - Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan City, Taiwan. FAU - Chung, Wen-Hung AU - Chung WH AD - Xiamen Chang Gung Allergology Consortium, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Medical Research Center, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Department of Dermatology, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Department of Dermatology and Drug Hypersensitivity Clinical and Research Center, Chang Gung Memorial Hospital, Linkou, Taipei and Keelung, Taiwan. AD - Cancer Vaccine and Immune Cell Therapy Core Laboratory, Department of Medical Research, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan. AD - Whole-Genome Research Core Laboratory of Human Diseases, Chang Gung Memorial Hospital, Keelung, Taiwan. FAU - Chen, Liang AU - Chen L AD - Xiamen Chang Gung Allergology Consortium, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Department of Respiratory and Critical Care Medicine, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Department of Allergy and Immunology, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. FAU - Chang, Chih-Jung AU - Chang CJ AD - Xiamen Chang Gung Allergology Consortium, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Medical Research Center, Xiamen Chang Gung Hospital, Xiamen, Fujian, China. AD - Department of Dermatology and Drug Hypersensitivity Clinical and Research Center, Chang Gung Memorial Hospital, Linkou, Taipei and Keelung, Taiwan. AD - School of Medicine, Huaqiao University, Quanzhou, Fujian, China. LA - eng PT - Journal Article DEP - 20220806 PL - United States TA - World Allergy Organ J JT - The World Allergy Organization journal JID - 101481283 PMC - PMC9386106 OTO - NOTNLM OT - 16S rRNA sequencing OT - AR, Allergic rhinitis OT - ASV, Amplicon sequence variant OT - Allergic rhinitis OT - EVs, Extracellular vesicles OT - Extracellular vesicle OT - FITC, Fluorescein isothiocyanate OT - GPI, Glycosylphosphatidylinositol OT - HCs, Healthy controls OT - HDM, House dust mite OT - KEGG, Kyoto Encyclopedia of Genes and Genomes OT - LEfSe, Linear discriminant analysis (LDA) effect size OT - LPS, Lipopolysaccharide OT - MAMPs, Microorganism-associated molecular patterns OT - MRPP, Multiple response permutation procedure OT - Microbiota OT - OMVs, Outer membrane vesicles OT - PBS, Phosphate-buffered saline OT - PCoA, Principal coordinate analysis OT - PLS-DA, Partial least squares discriminant analysis OT - PRRs, Pattern recognition receptors OT - TEM, Transmission electron microscopy OT - UPGMA, Unweighted pair-group method with arithmetic means EDAT- 2022/08/27 06:00 MHDA- 2022/08/27 06:01 PMCR- 2022/08/06 CRDT- 2022/08/26 02:25 PHST- 2022/03/31 00:00 [received] PHST- 2022/06/22 00:00 [revised] PHST- 2022/07/05 00:00 [accepted] PHST- 2022/08/26 02:25 [entrez] PHST- 2022/08/27 06:00 [pubmed] PHST- 2022/08/27 06:01 [medline] PHST- 2022/08/06 00:00 [pmc-release] AID - S1939-4551(22)00050-3 [pii] AID - 100674 [pii] AID - 10.1016/j.waojou.2022.100674 [doi] PST - epublish SO - World Allergy Organ J. 2022 Aug 6;15(8):100674. doi: 10.1016/j.waojou.2022.100674. eCollection 2022 Aug.