PMID- 36017468
OWN - NLM
STAT- MEDLINE
DCOM- 20220829
LR  - 20220831
IS  - 1537-744X (Electronic)
IS  - 2356-6140 (Print)
IS  - 1537-744X (Linking)
VI  - 2022
DP  - 2022
TI  - Diagnostic Performance of Three ELISAs for Detection of Antibodies against 
      SARS-CoV-2 in Human Samples.
PG  - 7754329
LID - 10.1155/2022/7754329 [doi]
LID - 7754329
AB  - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection that 
      causes coronavirus disease 2019 (COVID-19) is a disease with a high rate of 
      transmission. Serological tests are important to perform surveys and to determine 
      the immunological status of the population. Based on this, we evaluated three 
      enzyme-linked immunoassays (ELISAs) using different antigens from SARS-CoV-2 in a 
      cohort of 161 patients. The performance of the ELISA developed for immunoglobulin 
      G (IgG) measurement against SARS-CoV-2 was evaluated based on sensitivity, 
      specificity, and accuracy. We found specificities of 0.98, 0.98, and 0.99 and 
      sensitivities of 0.99, 0.91, and 0.87 for the nucleocapsid (N) protein, spike 
      protein, and receptor binding domain (RBD) fraction, respectively. The accuracy 
      assessment indicated the N protein (accuracy = 0.98) as the antigen most likely 
      to give a correct diagnosis. Overall, the antibody responses were present for all 
      three proteins in subjects with confirmed SARS-CoV-2 infections, showing a 
      similar pattern of antibody production for different antigens. In summary, these 
      highly sensitive and specific ELISAs, with a more competitive price, appear to be 
      a valid approach for the serodiagnosis of COVID-19.
CI  - Copyright (c) 2022 Cassio Meira et al.
FAU - Meira, Cassio
AU  - Meira C
AUID- ORCID: 0000-0002-7578-3615
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
FAU - Silva, Dahara
AU  - Silva D
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
FAU - Santos, Ivanilson
AU  - Santos I
AUID- ORCID: 0000-0002-2331-6150
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
FAU - Barreto, Breno
AU  - Barreto B
AUID- ORCID: 0000-0002-9897-2443
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
FAU - Rocha, Vinicius
AU  - Rocha V
AUID- ORCID: 0000-0002-5309-5950
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
FAU - Santos, Emanuelle
AU  - Santos E
AUID- ORCID: 0000-0002-5213-8197
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
FAU - Dos Reis, Bruna
AU  - Dos Reis B
AUID- ORCID: 0000-0002-3863-7483
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
FAU - Evangelista, Afranio
AU  - Evangelista A
AUID- ORCID: 0000-0001-8011-4128
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
FAU - Ribeiro Dos Santos, Ricardo
AU  - Ribeiro Dos Santos R
AUID- ORCID: 0000-0001-9216-0498
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
FAU - Machado, Bruna
AU  - Machado B
AUID- ORCID: 0000-0003-1655-0325
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
FAU - Ribeiro, Guilherme
AU  - Ribeiro G
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
AD  - School of Medicine, Federal University of Bahia (UFBA), Salvador, Brazil.
FAU - Badaro, Roberto
AU  - Badaro R
AUID- ORCID: 0000-0002-2861-5061
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
FAU - Soares, Milena
AU  - Soares M
AUID- ORCID: 0000-0001-7549-2992
AD  - SENAI Institute of Innovation in Health Advanced Systems (CIMATEC ISI SAS), 
      University Center SENAI/CIMATEC, Salvador, Brazil.
AD  - Goncalo Moniz Institute Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, 
      Brazil.
LA  - eng
PT  - Journal Article
DEP - 20220816
PL  - United States
TA  - ScientificWorldJournal
JT  - TheScientificWorldJournal
JID - 101131163
RN  - 0 (Antibodies, Viral)
RN  - 0 (Spike Glycoprotein, Coronavirus)
RN  - 0 (spike protein, SARS-CoV-2)
SB  - IM
MH  - Antibodies, Viral
MH  - *COVID-19/diagnosis/immunology
MH  - COVID-19 Testing
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - *SARS-CoV-2/immunology
MH  - Sensitivity and Specificity
MH  - Serologic Tests
MH  - Spike Glycoprotein, Coronavirus
PMC - PMC9398874
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as potential 
      conflicts of interest.
EDAT- 2022/08/27 06:00
MHDA- 2022/08/30 06:00
PMCR- 2022/08/16
CRDT- 2022/08/26 02:34
PHST- 2022/02/04 00:00 [received]
PHST- 2022/05/15 00:00 [revised]
PHST- 2022/05/30 00:00 [accepted]
PHST- 2022/08/26 02:34 [entrez]
PHST- 2022/08/27 06:00 [pubmed]
PHST- 2022/08/30 06:00 [medline]
PHST- 2022/08/16 00:00 [pmc-release]
AID - 10.1155/2022/7754329 [doi]
PST - epublish
SO  - ScientificWorldJournal. 2022 Aug 16;2022:7754329. doi: 10.1155/2022/7754329. 
      eCollection 2022.