PMID- 36018448 OWN - NLM STAT- MEDLINE DCOM- 20230127 LR - 20230202 IS - 1436-3771 (Electronic) IS - 1432-6981 (Linking) VI - 27 IP - 1 DP - 2023 Jan TI - Enhanced bone tissue regeneration with hydrogel-based scaffolds by embedding parathyroid hormone in mesoporous bioactive glass. PG - 125-137 LID - 10.1007/s00784-022-04696-3 [doi] AB - OBJECTIVES: To evaluate hydrogel-based scaffolds embedded with parathyroid hormone (PTH)-loaded mesoporous bioactive glass (MBG) on the enhancement of bone tissue regeneration in vitro. MATERIALS AND METHODS: MBG was produced via sol-gel technique followed by PTH solution imbibition. PTH-loaded MBG was blended into the hydrogels and submitted to a lyophilisation process associated with a chemical crosslinking reaction to the production of the scaffolds. Characterisation of the MBG and PTH-loaded MBG scaffolds, including the scanning electron microscope (SEM) connected with an X-ray detector (EDX), Fourier transform infrared (FTIR), compression strength, rheological measurements, swelling and degradation rates, and PTH release analysis, were performed. Also, bioactivity using simulated-body fluid (SBF), biocompatibility (MTT), and osteogenic differentiation analyses (von Kossa and Alizarin Red stainings, and mu-computed tomography, muCT) of the scaffolds were carried out. RESULTS: SEM images demonstrated MBG particles dispersed into the hydrogel-based scaffold structure, which was homogeneously porous and well interconnected. EDX and FTIR revealed large amounts of carbon, oxygen, sodium, and silica in the scaffold composition. Bioactivity experiments revealed changes on sample surfaces over the analysed period, indicating the formation of carbonated hydroxyapatite; however, the chemical composition remained stable. PTH-loaded hydrogel-based scaffolds were biocompatible for stem cells from human-exfoliated deciduous teeth (SHED). A high quantity of calcium deposits on the extracellular matrix of SHED was found for PTH-loaded hydrogel-based scaffolds. muCT images showed MBG particles dispersed into the scaffolds' structure, and a porous, lamellar, and interconnected hydrogel architecture. CONCLUSIONS: PTH-loaded hydrogel-based scaffolds demonstrated consistent morphology and physicochemical properties for bone tissue regeneration, as well as bioactivity, biocompatibility, and osteoinductivity in vitro. Thus, the scaffolds presented here are recommended for future studies on 3D printing. CLINICAL RELEVANCE: Bone tissue regeneration is still a challenge for several approaches to oral and maxillofacial surgeries, though tissue engineering applying SHED, scaffolds, and osteoinductive mediators might help to overcome this clinical issue. CI - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Sordi, Mariane Beatriz AU - Sordi MB AUID- ORCID: 0000-0001-7873-0765 AD - Centre for Dental Implants Research (CEPID), Federal University of Santa Catarina (UFSC), Campus Trindade, Florianopolis, SC, Brazil. AD - Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, Guy's Hospital, King's College London, London, UK. AD - Applied Virology Laboratory (LVA), Federal University of Santa Catarina (UFSC), Campus Trindade, Florianopolis, SC, Brazil. FAU - Fredel, Marcio Celso AU - Fredel MC AUID- ORCID: 0000-0001-7020-762X AD - Ceramic and Composite Materials Research Group (CERMAT), Federal University of Santa Catarina (UFSC), Campus Trindade, Florianopolis, SC, Brazil. FAU - da Cruz, Ariadne Cristiane Cabral AU - da Cruz ACC AUID- ORCID: 0000-0001-7306-4708 AD - Centre for Dental Implants Research (CEPID), Federal University of Santa Catarina (UFSC), Campus Trindade, Florianopolis, SC, Brazil. Ariadne.cruz@ufsc.br. AD - Applied Virology Laboratory (LVA), Federal University of Santa Catarina (UFSC), Campus Trindade, Florianopolis, SC, Brazil. Ariadne.cruz@ufsc.br. FAU - Sharpe, Paul Thomas AU - Sharpe PT AUID- ORCID: 0000-0003-2116-9561 AD - Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, Guy's Hospital, King's College London, London, UK. FAU - de Souza Magini, Ricardo AU - de Souza Magini R AUID- ORCID: 0000-0002-6380-8870 AD - Centre for Dental Implants Research (CEPID), Federal University of Santa Catarina (UFSC), Campus Trindade, Florianopolis, SC, Brazil. LA - eng GR - 1113_2015/International Team for Implantology/ PT - Journal Article DEP - 20220826 PL - Germany TA - Clin Oral Investig JT - Clinical oral investigations JID - 9707115 RN - 0 (Parathyroid Hormone) RN - 0 (Hydrogels) RN - 0 (Biocompatible Materials) MH - Humans MH - *Tissue Scaffolds/chemistry MH - *Osteogenesis MH - Parathyroid Hormone/pharmacology MH - Hydrogels/pharmacology MH - Bone Regeneration MH - Glass/chemistry MH - Porosity MH - Biocompatible Materials/chemistry OTO - NOTNLM OT - Bioactive glass OT - Bone regeneration OT - Hydrogel OT - Parathyroid hormone OT - Stem cells from human-exfoliated deciduous teeth EDAT- 2022/08/27 06:00 MHDA- 2023/01/28 06:00 CRDT- 2022/08/26 11:19 PHST- 2022/03/18 00:00 [received] PHST- 2022/08/18 00:00 [accepted] PHST- 2022/08/27 06:00 [pubmed] PHST- 2023/01/28 06:00 [medline] PHST- 2022/08/26 11:19 [entrez] AID - 10.1007/s00784-022-04696-3 [pii] AID - 10.1007/s00784-022-04696-3 [doi] PST - ppublish SO - Clin Oral Investig. 2023 Jan;27(1):125-137. doi: 10.1007/s00784-022-04696-3. Epub 2022 Aug 26.