PMID- 36028098
OWN - NLM
STAT- MEDLINE
DCOM- 20221101
LR  - 20230112
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 110
DP  - 2022 Dec
TI  - Intermittent fasting attenuates lipopolysaccharide-induced acute lung injury in 
      mice by modulating macrophage polarization.
PG  - 109133
LID - S0955-2863(22)00201-7 [pii]
LID - 10.1016/j.jnutbio.2022.109133 [doi]
AB  - Acute lung injury (ALI) is a spectrum of acute and life-threatening pulmonary 
      inflammatory conditions. Treatment of ALI remains a clinical challenge. Recently, 
      intermittent fasting (IF) has been shown to improve health and alleviate many 
      diseases. In this study, we tested whether IF attenuated ALI and investigated the 
      mechanism underlying this process. In vivo, the effects of IF on ALI were 
      evaluated in a lipopolysaccharide (LPS)-induced murine ALI model. We found that 
      two times of 24-h fasting in a week before ALI efficiently ameliorated 
      LPS-induced lung injury in mice, characterized by alleviated lung lesions, 
      wet-to-dry weight ratio, myeloperoxidase activity, malondialdehyde content, and 
      lower levels of tumor necrosis factor-alpha, interleukin-6, and interleukin-1beta. In 
      vitro, functional assays were conducted to assess IF on the inflammatory response 
      and macrophage polarization of bone marrow-derived macrophages (BMDMs) treated 
      with LPS or IL-4. And PPARgamma antagonist GW9662 and AMPK siRNA were used to test 
      the role of PPARgamma and AMPK in the IF-mediated improvement of ALI. The results 
      showed that IF (serum deprivation) suppressed macrophage M1 activation and 
      promoted M2 activation in LPS-treated BMDMs. While, IF also augmented macrophage 
      M2 polarization in IL-4-treated BMDMs. Further mechanistic studies showed that 
      the promotive effect of IF on M2 polarization was related to the activation of 
      the PPARgamma and AMPK pathways. In conclusion, this study suggests that IF enhances 
      M2 polarization by activating the AMPK and PPARgamma pathways, thus facilitating 
      anti-inflammatory response and ameliorating ALI.
CI  - Copyright (c) 2022. Published by Elsevier Inc.
FAU - Ma, Li
AU  - Ma L
AD  - Department of Anesthesiology, Liuzhou People's Hospital, Liuzhou, Guangxi, 
      545006, China.
FAU - Chen, Yan-Qing
AU  - Chen YQ
AD  - Department of Anesthesiology, Liuzhou People's Hospital, Liuzhou, Guangxi, 
      545006, China.
FAU - You, Zhi-Jian
AU  - You ZJ
AD  - Department of Anesthesiology, Liuzhou People's Hospital, Liuzhou, Guangxi, 
      545006, China.
FAU - Jiang, Zhong-Sheng
AU  - Jiang ZS
AD  - Department of Infection, Liuzhou People's Hospital, Liuzhou, Guangxi, 545001, 
      China.
FAU - Fang, Yu
AU  - Fang Y
AD  - Medical laboratory and Pathology Center, The First Hospital of Hunan University 
      of Chinese Medicine, Changsha, Hunan, 410021, China. Electronic address: 
      fangyu.stone@163.com.
FAU - Dong, Liang
AU  - Dong L
AD  - Department of Anesthesiology, Liuzhou People's Hospital, Liuzhou, Guangxi, 
      545006, China. Electronic address: donglqqsx2008@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220824
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Lipopolysaccharides)
RN  - 0 (PPAR gamma)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Lipopolysaccharides/metabolism
MH  - Fasting
MH  - PPAR gamma/metabolism
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Interleukin-4/metabolism/pharmacology/therapeutic use
MH  - *Acute Lung Injury/chemically induced
MH  - Macrophages/metabolism
MH  - Lung
OTO - NOTNLM
OT  - AMPK
OT  - Acute lung injury
OT  - PPARgamma
OT  - intermittent fasting
OT  - macrophage polarization
EDAT- 2022/08/27 06:00
MHDA- 2022/11/02 06:00
CRDT- 2022/08/26 19:25
PHST- 2022/01/09 00:00 [received]
PHST- 2022/05/14 00:00 [revised]
PHST- 2022/08/02 00:00 [accepted]
PHST- 2022/08/27 06:00 [pubmed]
PHST- 2022/11/02 06:00 [medline]
PHST- 2022/08/26 19:25 [entrez]
AID - S0955-2863(22)00201-7 [pii]
AID - 10.1016/j.jnutbio.2022.109133 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2022 Dec;110:109133. doi: 10.1016/j.jnutbio.2022.109133. Epub 
      2022 Aug 24.