PMID- 36029491
OWN - NLM
STAT- MEDLINE
DCOM- 20220830
LR  - 20220830
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 68
IP  - 5
DP  - 2022 May 31
TI  - Effects of Atractylon on Proliferation and Apoptosis of Intestinal Cancer Cells 
      Through PI3K/AKT/mTOR Signaling Pathway.
PG  - 153-160
LID - 10.14715/cmb/2022.68.5.21 [doi]
AB  - The study aimed to explore the effects of atractylon on the proliferation and 
      apoptosis of intestinal cancer cells through the phosphatidylinositol 3-hydroxy 
      kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) 
      signaling pathway. The intestinal cancer HT29 cell lines were cultured in vitro, 
      and atractylon at different concentrations (15 and 30 mg/mL) was added. Then cell 
      proliferative activity was detected via cell counting kit-8 (CCK8) assay, and the 
      proportion of positive cells was determined using EdU staining. The content of 
      interferon-gamma (INF-gamma), tumor necrosis factor-alpha (TNF-alpha) and matrix 
      metalloproteinase-9 (MMP-9) was detected via enzyme-linked immunosorbent assay 
      (ELISA), and the apoptosis of HT29 cells was detected through terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. 
      Moreover, reverse transcription-polymerase chain reaction (RT-PCR) was performed 
      to determine the messenger ribonucleic acid (mRNA) levels of proliferation, 
      apoptosis and PI3K/AKT/mTOR signaling pathway-related genes, and Western blotting 
      was used to analyze the expression of the PI3K/AKT/mTOR signaling pathway. The 
      cell growth status was poorer with a lower density in the 15 mg/mL atractylon 
      group and basically normal morphological structure in the 30 mg/mL atractylon 
      group. The number of cells significantly declined and the proliferative activity 
      was also significantly weakened in the 30 mg/mL atractylon group. There were 
      obviously more apoptotic cells in the 30 mg/mL atractylon group. Besides, INF-gamma, 
      TNF-alpha and MMP-9 were all evidently decreased in the 30 mg/mL atractylon group. 
      Expressions of B-cell lymphoma-2 (Bcl-2), PI3K, AKT and mTOR obviously declined 
      in the 30 mg/mL atractylon group, and they were raised in the NC group, while the 
      expression of Caspase3 showed the opposite trends. Atractylon at an appropriate 
      concentration can inhibit the proliferation and promote the apoptosis of 
      intestinal cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway, which 
      can be used to treat colorectal cancer and other related diseases.
FAU - Mao, Junjun
AU  - Mao J
AD  - Department of Oncology, Yantai Hospital of Traditional Chinese Medicine, 
      Shandong, 264001, China. humian106275451@163.com.
FAU - Wang, Xinping
AU  - Wang X
AD  - Department of Clinical Laboratory, Yantai Hospital of Traditional Chinese 
      Medicine, Shandong, 264001, China. humian106275451@163.com.
FAU - Yu, Minghui
AU  - Yu M
AD  - Department of Clinical Laboratory, Yantai Wanhua Hospital, Shandong, 264001, 
      China. humian106275451@163.com.
FAU - Sun, Chenkun
AU  - Sun C
AD  - Department of General Surgery, Yantai Hospital of Traditional Chinese Medicine, 
      Shandong, 264001, China. sunlight823@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20220531
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Sesquiterpenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 6989-21-5 (atractylon)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Humans
MH  - *Intestinal Neoplasms
MH  - Matrix Metalloproteinase 9
MH  - Phosphatidylinositol 3-Kinase
MH  - Phosphatidylinositol 3-Kinases
MH  - *Proto-Oncogene Proteins c-akt
MH  - Sesquiterpenes
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases
MH  - Tumor Necrosis Factor-alpha
EDAT- 2022/08/28 06:00
MHDA- 2022/08/31 06:00
CRDT- 2022/08/27 14:11
PHST- 2022/05/18 00:00 [received]
PHST- 2022/08/27 14:11 [entrez]
PHST- 2022/08/28 06:00 [pubmed]
PHST- 2022/08/31 06:00 [medline]
AID - 10.14715/cmb/2022.68.5.21 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2022 May 31;68(5):153-160. doi: 
      10.14715/cmb/2022.68.5.21.