PMID- 36030735
OWN - NLM
STAT- MEDLINE
DCOM- 20221110
LR  - 20221110
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 252
DP  - 2023 Jan 15
TI  - Coumarin-based derivatization reagent for LC-MS analysis of amino acids.
PG  - 123730
LID - S0039-9140(22)00526-4 [pii]
LID - 10.1016/j.talanta.2022.123730 [doi]
AB  - In this paper, we report about the application of a sensitive fluorescent 
      derivatization reagent Coumarin151-N-Hydroxysuccinimidyl Carbamate (Cou151DSC) 
      for amino compounds using high-performance liquid chromatography (HPLC) 
      compatible with ultraviolet (UV), fluorescence detector (FLD) and electrospray 
      ionization - tandem mass spectrometry (ESI-MS/MS)-positive mode. We optimized 
      derivatization procedure and validated an analytical method to determine 24 amino 
      acids in Kvass drink using Norvaline as an internal standard. Compared to 
      6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate (6-AQC), the derivatization with 
      Cou151 DSC is faster and milder, for 5 min at 40 degrees C instead of 15 min at 55 degrees C. The 
      limit of quantitation (LOQ, pmol on column) for 21 amino acids in this work is 
      lower 1.1-30.0 times than values obtained with 6-AQC. The derivatives have 
      excitation wavelength at 355 nm and emission wavelength at 486 nm. Their MS/MS 
      fragmentation behaviors were examined together with 23 other amino compounds. We 
      found three possibilities to lose a neutral group which can be Coumarin 151 
      isocyanate Cou151NCO (255 Da), amine Coumarin 151 (229 Da) or urea Cou151CONH(2) 
      (272 Da). The accuracy of the proposed method was within 83-107% with good 
      relative standard deviations (RSDs) of equal or less than 6%. The recoveries were 
      from 82 to 120% in four spiked concentrations, repeatability was between 0 and 
      14%. The intra- and inter-day precision are less than 13% and 18%, respectively.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Bui, Nguyen Kim Ngan
AU  - Bui NKN
AD  - University of Tartu, Institute of Chemistry, Ravila 14a, Tartu 50411, Estonia. 
      Electronic address: nguyen.bui@ut.ee.
FAU - Selberg, Sigrid
AU  - Selberg S
AD  - University of Tartu, Institute of Chemistry, Ravila 14a, Tartu 50411, Estonia.
FAU - Herodes, Koit
AU  - Herodes K
AD  - University of Tartu, Institute of Chemistry, Ravila 14a, Tartu 50411, Estonia.
FAU - Leito, Ivo
AU  - Leito I
AD  - University of Tartu, Institute of Chemistry, Ravila 14a, Tartu 50411, Estonia.
LA  - eng
PT  - Journal Article
DEP - 20220805
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Amino Acids)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Amines)
RN  - 0 (Coumarins)
SB  - IM
MH  - Chromatography, Liquid/methods
MH  - *Amino Acids/analysis
MH  - *Tandem Mass Spectrometry
MH  - Indicators and Reagents
MH  - Chromatography, High Pressure Liquid/methods
MH  - Amines
MH  - Coumarins/analysis
OTO - NOTNLM
OT  - Amino acids
OT  - Cou151DSC
OT  - Coumarin151-N-Hydroxysuccinimidyl Carbamate
OT  - Derivatization
OT  - ESI
OT  - LC-MS
OT  - MS/MS
OT  - Neutral loss
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/08/29 06:00
MHDA- 2022/11/11 06:00
CRDT- 2022/08/28 18:20
PHST- 2022/03/06 00:00 [received]
PHST- 2022/07/04 00:00 [revised]
PHST- 2022/07/06 00:00 [accepted]
PHST- 2022/08/29 06:00 [pubmed]
PHST- 2022/11/11 06:00 [medline]
PHST- 2022/08/28 18:20 [entrez]
AID - S0039-9140(22)00526-4 [pii]
AID - 10.1016/j.talanta.2022.123730 [doi]
PST - ppublish
SO  - Talanta. 2023 Jan 15;252:123730. doi: 10.1016/j.talanta.2022.123730. Epub 2022 
      Aug 5.