PMID- 36032674
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 2296-889X (Print)
IS  - 2296-889X (Electronic)
IS  - 2296-889X (Linking)
VI  - 9
DP  - 2022
TI  - Increased expressions of CD123, CD63, CD203c, and Fc epsilon receptor I on blood 
      leukocytes of allergic asthma.
PG  - 907092
LID - 10.3389/fmolb.2022.907092 [doi]
LID - 907092
AB  - Background : Altered basophil identification markers have been discovered to 
      associate with allergic asthma (AA) in recent years. However, little is known 
      about the expression of basophil markers in blood granulocytes. Aim: To parallel 
      test blood basophils in peripheral blood mononuclear cell (PBMC) and granulocyte 
      populations of patients with AA and AA combined with allergic rhinitis (ARA) 
      Methods: The expressions of surface molecules were determined via flow cytometry. 
      CD123 expressing cells in blood were isolated using a cell sorting technique, and 
      mouse AA models were employed for in vivo study. Results: The numbers of 
      CD123(+)HLA-DR(-) cells in the granulocytes of AA and ARA patients markedly 
      increased. However, only 49.7% of CD123(+)HLA-DR(-) cells in granulocytes and 
      99.0% of CD123(+)HLA-DR(-) cells in PBMCs were basophils. Almost all 
      CD123(+)HLA-DR(-) cells expressed CD63 regardless in granulocytes or PBMC. The 
      numbers of CD63, Fc epsilon receptor I (FcepsilonRI), and CD203c expressing cells 
      markedly enhanced in CD123(+)HLA-DR(-) granulocytes of AA and ARA patients. Mean 
      fluorescence intensity (MFI) of CD63 and CD203c expressions on CD123(+)HLA-DR(-) 
      PBMC and granulocytes of AA and ARA patients dramatically elevated. House dust 
      mite extract (HDME) and Artemisia sieversiana wild allergen extract (ASWE) 
      enhanced the numbers of CD63(+)CD123(+)HLA-DR(-) granulocytes and PBMC and the 
      MFI of CD203c expression on CD123(+)HLA-DR(-) granulocyte of AA and ARA patients. 
      Histamine, tryptase, and PGD2 enhanced proportions of CD123(+) KU812 cells. ASWE- 
      and HDME-induced AA mice showed upregulated CD63 expression on basophils. In 
      conclusion, upregulated expressions of CD123, CD203c, CD63, and FcepsilonRIalpha in PBMC 
      and granulocytes of patients with AA and ARA suggest that CD123(+)HLA-DR(-) cells 
      may contribute to the development of AA and ARA.
CI  - Copyright (c) 2022 Xie, Chen, Zhang, Wang, Zang, Zhan, Gu, Wang and He.
FAU - Xie, Hua
AU  - Xie H
AD  - The PLA Center of Respiratory and Allergic Disease Diagnosing Management, General 
      Hospital of Northern Theater Command, Shenyang, China.
AD  - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Chen, Liping
AU  - Chen L
AD  - Translational Medicine Institute, Shenyang Medical College, Shenyang, China.
AD  - Department of Respiratory and Critical Care Medicine, The Second Hospital of 
      Senyang Medical College, Shenyang, China.
FAU - Zhang, Huiyun
AU  - Zhang H
AD  - Translational Medicine Institute, Shenyang Medical College, Shenyang, China.
FAU - Wang, Junling
AU  - Wang J
AD  - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Zang, Yanyan
AU  - Zang Y
AD  - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Zhan, Mengmeng
AU  - Zhan M
AD  - Translational Medicine Institute, Shenyang Medical College, Shenyang, China.
FAU - Gu, Fangqiu
AU  - Gu F
AD  - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
FAU - Wang, Shunlan
AU  - Wang S
AD  - Central Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, 
      Central South University, Haikou, China.
FAU - He, Shaoheng
AU  - He S
AD  - The PLA Center of Respiratory and Allergic Disease Diagnosing Management, General 
      Hospital of Northern Theater Command, Shenyang, China.
AD  - Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of 
      Jinzhou Medical University, Jinzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220811
PL  - Switzerland
TA  - Front Mol Biosci
JT  - Frontiers in molecular biosciences
JID - 101653173
PMC - PMC9403487
OTO - NOTNLM
OT  - CD123
OT  - CD203c
OT  - CD63
OT  - FcepsilonRI
OT  - basophil
OT  - granulocyte
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as potential 
      conflicts of interest.
EDAT- 2022/08/30 06:00
MHDA- 2022/08/30 06:01
PMCR- 2022/01/01
CRDT- 2022/08/29 04:44
PHST- 2022/03/29 00:00 [received]
PHST- 2022/06/27 00:00 [accepted]
PHST- 2022/08/29 04:44 [entrez]
PHST- 2022/08/30 06:00 [pubmed]
PHST- 2022/08/30 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 907092 [pii]
AID - 10.3389/fmolb.2022.907092 [doi]
PST - epublish
SO  - Front Mol Biosci. 2022 Aug 11;9:907092. doi: 10.3389/fmolb.2022.907092. 
      eCollection 2022.