PMID- 36033670
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 7
IP  - 33
DP  - 2022 Aug 23
TI  - Effects of Detergents on the Spectral Features of B820 Bacteriochlorophyll a in 
      Light-Harvesting Complex 3.
PG  - 29231-29235
LID - 10.1021/acsomega.2c03323 [doi]
AB  - Excitonic coupling of bacteriochlorophyll (BChl) a in light-harvesting (LH) 
      proteins of purple photosynthetic bacteria is key for efficient photon capture 
      and energy transfer. Environmental factors can affect the spectral features of 
      these BChl a pigments and investigating these effects can provide insight into 
      the molecular mechanisms underlying the photosynthetic spectral tuning. The 
      present study analyzes the spectral alterations of the Q(y) band of B820 BChl a 
      within the LH3 protein in relation to the type and concentration of detergents in 
      the buffer. Changing the detergent from lauryl dimethylamine N-oxide (LDAO) to 
      n-dodecyl-beta-d-maltoside (DDM) caused a red shift in the B820 Q(y) band 
      accompanied by hyperchromism; these spectral alterations were completely reversed 
      by exchanging back from DDM to LDAO. These results reflect the different effects 
      of harsh vs mild detergents on the perturbation of LH3. The B820 Q(y) band did 
      not change when LDAO or NaCl concentration was altered, suggesting that 
      electrostatic effects by external components have little influence on the 
      spectral features of B820 BChl a in LH3.
CI  - (c) 2022 The Authors. Published by American Chemical Society.
FAU - Saga, Yoshitaka
AU  - Saga Y
AUID- ORCID: 0000-0001-7384-9169
AD  - Department of Chemistry, Faculty of Science and Engineering, Kindai University, 
      Higashi-Osaka, Osaka 577-8502, Japan.
FAU - Hamanishi, Kohei
AU  - Hamanishi K
AD  - Department of Chemistry, Faculty of Science and Engineering, Kindai University, 
      Higashi-Osaka, Osaka 577-8502, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220811
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC9404518
COIS- The authors declare no competing financial interest.
EDAT- 2022/08/30 06:00
MHDA- 2022/08/30 06:01
PMCR- 2022/08/11
CRDT- 2022/08/29 04:57
PHST- 2022/05/28 00:00 [received]
PHST- 2022/08/03 00:00 [accepted]
PHST- 2022/08/29 04:57 [entrez]
PHST- 2022/08/30 06:00 [pubmed]
PHST- 2022/08/30 06:01 [medline]
PHST- 2022/08/11 00:00 [pmc-release]
AID - 10.1021/acsomega.2c03323 [doi]
PST - epublish
SO  - ACS Omega. 2022 Aug 11;7(33):29231-29235. doi: 10.1021/acsomega.2c03323. 
      eCollection 2022 Aug 23.