PMID- 36034652
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 2296-2646 (Print)
IS  - 2296-2646 (Electronic)
IS  - 2296-2646 (Linking)
VI  - 10
DP  - 2022
TI  - Development of a reliable UHPLC-MS/MS method for simultaneous determination of 
      zearalenone and zearalenone-14-glucoside in various feed products.
PG  - 955266
LID - 10.3389/fchem.2022.955266 [doi]
LID - 955266
AB  - A reliable ultra-high-performance liquid chromatography-tandem mass spectrometry 
      method (UHPLC-MS/MS) was developed for the simultaneous determination of two 
      mycotoxins, that is, zearalenone (ZEN) and zearalenone-14-glucoside (ZEN-14G) in 
      formula feed, concentrated feed, and premixed feed products. An improved sample 
      pretreatment was achieved with the hydrophilic-lipophilic balance (HLB) 
      cartridges efficiently removing the impurities and enriching the target analytes 
      in different feeds. The critical parameters affecting the performance of the 
      solid-phase extraction (SPE) procedure were carefully optimized, and 20% 
      acetonitrile in water as the loading solution, 50% methanol in water as the 
      washing solvent, and 5 ml of methanol as the elution solvent yielded the optimal 
      purification efficiencies. The established method was thoroughly validated in 
      terms of linearity (R (2) >/= 0.999), sensitivity (limit of quantification in the 
      range of 0.50-5.00 mug kg(-1)), recovery (89.35 +/- 2.67% to 110.93 +/- 1.56%), and 
      precision (RSD, 3.00-14.20%), and it was then successfully applied to investigate 
      a total of 60 feed samples. Among them, 50 samples were found to be contaminated 
      with ZEN (an incidence of 83.3%) at levels ranging from 0.63 to 615.24 mug kg(-1), 
      whereas 22 samples were contaminated with ZEN-14G (an incidence of 36.7%) in the 
      range of 0.89-15.31 mug kg(-1). The developed method proved to be a specific and 
      reliable tool for intensive monitoring of ZEN and ZEN-14G in complex feed 
      matrices.
CI  - Copyright (c) 2022 Zhang, Cai, Fan, Huang, Zhao, Cao, Zhao, Tangni and Han.
FAU - Zhang, Zhiqi
AU  - Zhang Z
AD  - Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of 
      Agricultural Sciences, Shanghai, China.
FAU - Cai, Yaling
AU  - Cai Y
AD  - Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of 
      Agricultural Sciences, Shanghai, China.
FAU - Fan, Kai
AU  - Fan K
AD  - Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of 
      Agricultural Sciences, Shanghai, China.
FAU - Huang, Qingwen
AU  - Huang Q
AD  - Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of 
      Agricultural Sciences, Shanghai, China.
FAU - Zhao, Xiuying
AU  - Zhao X
AD  - Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of 
      Agricultural Sciences, Shanghai, China.
FAU - Cao, Haojie
AU  - Cao H
AD  - Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of 
      Agricultural Sciences, Shanghai, China.
FAU - Zhao, Zhihui
AU  - Zhao Z
AD  - Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of 
      Agricultural Sciences, Shanghai, China.
FAU - Tangni, Emmanuel K
AU  - Tangni EK
AD  - Organic Contaminants and Additives, Chemical and Physical Health Risks, Brussels, 
      Belgium.
FAU - Han, Zheng
AU  - Han Z
AD  - Institute for Agro-Food Standards and Testing Technology, Shanghai Academy of 
      Agricultural Sciences, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20220810
PL  - Switzerland
TA  - Front Chem
JT  - Frontiers in chemistry
JID - 101627988
PMC - PMC9399508
OTO - NOTNLM
OT  - HLB cartridges
OT  - feed products
OT  - ultra-high-performance liquid chromatography-tandem mass spectrometry
OT  - zearalenone
OT  - zearalenone-14-glucoside
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/30 06:00
MHDA- 2022/08/30 06:01
PMCR- 2022/01/01
CRDT- 2022/08/29 05:12
PHST- 2022/05/28 00:00 [received]
PHST- 2022/07/05 00:00 [accepted]
PHST- 2022/08/29 05:12 [entrez]
PHST- 2022/08/30 06:00 [pubmed]
PHST- 2022/08/30 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 955266 [pii]
AID - 10.3389/fchem.2022.955266 [doi]
PST - epublish
SO  - Front Chem. 2022 Aug 10;10:955266. doi: 10.3389/fchem.2022.955266. eCollection 
      2022.