PMID- 36035183
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220830
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 13
DP  - 2022
TI  - RNA sequencing and integrative analysis reveal pathways and hub genes associated 
      with TGFbeta1 stimulation on prostatic stromal cells.
PG  - 919103
LID - 10.3389/fgene.2022.919103 [doi]
LID - 919103
AB  - Objective: Benign prostatic hyperplasia (BPH) is the most common urological 
      disease in elderly men. The transforming growth factor beta 1 (TGFbeta1) plays an 
      important role in the proliferation and differentiation of BPH stroma. However, 
      it is not clear yet which important pathways and key genes are the downstream of 
      TGFbeta1 acting on prostatic stromal cells. Methods: GSE132714 is currently the 
      newer, available, and best high-throughput sequencing data set for BPH disease 
      and includes the largest number of BPH cases. We examined the TGFbeta1 expression 
      level in BPH and normal prostate (NP) by analyzing the GSE132714 data set as well 
      as carrying out immunohistochemistry of 15 BPH and 15 NP samples. Primary 
      prostatic stromal cells (PrSCs) were isolated from five fresh BPH tissues. RNA 
      sequencing and bioinformatics analysis were used to reveal important pathways and 
      hub genes associated with TGFbeta1 stimulation on PrSCs. Results: TGFbeta1 was 
      upregulated in BPH stroma compared to NP stroma. A total of 497 genes (244 
      upregulated and 253 downregulated) were differentially expressed in PrSCs with 
      and without TGFbeta1 stimulation. The Gene Ontology revealed that differentially 
      expressed genes (DEGs) were mainly enriched in progesterone secretion, 
      interleukin-7 receptor binding, and CSF1-CSF1R complex. The Wnt signaling 
      pathway, PI3K-Akt signaling pathway, JAK-STAT signaling pathway, and Hippo 
      signaling pathway were screened based on the Kyoto Encyclopedia of Genes and 
      Genomes (KEGG) analyses. FN1, SMAD3, CXCL12, VCAM1, and ICAM1 were selected as 
      hub genes according to the degree of connection from the protein-protein 
      interaction (PPI) network. Conclusion: This study sheds some new insights into 
      the role of TGFbeta1 in BPH stroma and provides some clues for the identification of 
      potential downstream mechanisms and targets.
CI  - Copyright (c) 2022 Xiang, Du, Wang, Liu, Yan, Hao, Liu, Guan and Ping.
FAU - Xiang, Peng
AU  - Xiang P
AD  - Department of Urology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
FAU - Du, Zhen
AU  - Du Z
AD  - Department of Urology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
FAU - Wang, Mingdong
AU  - Wang M
AD  - Department of Urology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
FAU - Liu, Dan
AU  - Liu D
AD  - Department of Urology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
FAU - Yan, Wei
AU  - Yan W
AD  - Department of Urology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
FAU - Hao, Yongxiu
AU  - Hao Y
AD  - Department of Epidemiology and Biostatistics, School of Public Health, Peking 
      University, Beijing, China.
FAU - Liu, Yutong
AU  - Liu Y
AD  - Department of Urology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
FAU - Guan, Di
AU  - Guan D
AD  - Department of Urology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
FAU - Ping, Hao
AU  - Ping H
AD  - Department of Urology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20220812
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC9412917
OTO - NOTNLM
OT  - RNA sequencing
OT  - TGFbeta1
OT  - benign prostatic hyperplasia
OT  - bioinformatics analysis
OT  - prostatic stromal cells
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/30 06:00
MHDA- 2022/08/30 06:01
PMCR- 2022/08/12
CRDT- 2022/08/29 05:20
PHST- 2022/04/13 00:00 [received]
PHST- 2022/06/29 00:00 [accepted]
PHST- 2022/08/29 05:20 [entrez]
PHST- 2022/08/30 06:00 [pubmed]
PHST- 2022/08/30 06:01 [medline]
PHST- 2022/08/12 00:00 [pmc-release]
AID - 919103 [pii]
AID - 10.3389/fgene.2022.919103 [doi]
PST - epublish
SO  - Front Genet. 2022 Aug 12;13:919103. doi: 10.3389/fgene.2022.919103. eCollection 
      2022.