PMID- 36041391
OWN - NLM
STAT- MEDLINE
DCOM- 20220928
LR  - 20220928
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 101
IP  - 10
DP  - 2022 Oct
TI  - Role of EtMIC4 EGF-like in regulating the apoptosis of Eimeria tenella host cells 
      via the EGFR pathway.
PG  - 102075
LID - S0032-5791(22)00366-2 [pii]
LID - 10.1016/j.psj.2022.102075 [doi]
LID - 102075
AB  - This study aimed to explore the role and key point of EtMIC4 EGF-like recombinant 
      protein in regulating the apoptosis of Eimeria tenella host cells via the 
      epidermal growth factor receptor (EGFR) pathway. The cells were treated with 
      EtMIC4 EGF-like protein, EGFR-specific siRNA, or both. Infection and apoptosis 
      rates as well as dynamic changes in the key genes and proteins of the EGFR 
      signaling pathway in the host cells were determined. Results showed that the E. 
      tenella and EtMIC4 EGF-like group had the highest infection rate (P < 0.01). In 
      cells treated with EtMIC4 EGF-like for 4 to 24 h, the apoptosis rate was 
      significantly decreased (P < 0.01) and the relative mRNA expression and protein 
      phosphorylation levels of EGFR, protein kinase B (AKT), and extracellular 
      regulated protein kinases (ERK) were significantly increased (P < 0.01). In E. 
      tenella sporozoites infected for 4 to 96 h, the rate of host cell apoptosis 
      induced by E. tenella infection was significantly (P < 0.01) reduced by EtMIC4 
      EGF-like. The relative mRNA expression and protein phosphorylation levels of 
      EGFR, AKT, and ERK in the host cells of E. tenella + EtMIC4 EGF-like group were 
      significantly increased (P < 0.01). These results indicated that E. tenella could 
      activate the EGFR pathway through EtMIC4 EGF-like and regulate the expression of 
      key genes in the AKT and ERK signaling pathways, thereby inhibiting cell 
      apoptosis.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Zhang, Xue-Song
AU  - Zhang XS
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China.
FAU - Zhao, Yong-Juan
AU  - Zhao YJ
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China; School of Food and Environment, Jinzhong College of Information, 
      Taigu, Jinzhong, 030801, China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China.
FAU - Xu, Tong
AU  - Xu T
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China.
FAU - Cui, Kai-Ling
AU  - Cui KL
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China.
FAU - Duan, Bu-Ting
AU  - Duan BT
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China.
FAU - Lv, Xiao-Ling
AU  - Lv XL
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China.
FAU - Zhang, Li
AU  - Zhang L
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China.
FAU - Xu, Zhi-Yong
AU  - Xu ZY
AD  - College of Animal Science and Veterinary Medicine, Henan Institute of Science and 
      Technology, Xinxiang 453003, China.
FAU - Bai, Rui
AU  - Bai R
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China.
FAU - Zheng, Ming-Xue
AU  - Zheng MX
AD  - College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, 
      030801, China. Electronic address: zhengmingxue288@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220726
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Recombinant Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Chickens/genetics
MH  - *Eimeria tenella/physiology
MH  - Epidermal Growth Factor/metabolism
MH  - ErbB Receptors/genetics
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Messenger/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Recombinant Proteins/metabolism
PMC - PMC9449671
OTO - NOTNLM
OT  - EGFR pathway
OT  - Eimeria tenella
OT  - EtMIC4 EGF-like
OT  - apoptosis
EDAT- 2022/08/31 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/07/26
CRDT- 2022/08/30 18:27
PHST- 2022/05/10 00:00 [received]
PHST- 2022/07/06 00:00 [revised]
PHST- 2022/07/19 00:00 [accepted]
PHST- 2022/08/31 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/08/30 18:27 [entrez]
PHST- 2022/07/26 00:00 [pmc-release]
AID - S0032-5791(22)00366-2 [pii]
AID - 102075 [pii]
AID - 10.1016/j.psj.2022.102075 [doi]
PST - ppublish
SO  - Poult Sci. 2022 Oct;101(10):102075. doi: 10.1016/j.psj.2022.102075. Epub 2022 Jul 
      26.