PMID- 36044708
OWN - NLM
STAT- MEDLINE
DCOM- 20221115
LR  - 20231102
IS  - 1522-1555 (Electronic)
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 323
IP  - 5
DP  - 2022 Nov 1
TI  - Carbohydrate intake in recovery from aerobic exercise differentiates skeletal 
      muscle microRNA expression.
PG  - E435-E447
LID - 10.1152/ajpendo.00110.2022 [doi]
AB  - Posttranscriptional regulation by microRNA (miRNA) facilitates exercise and 
      diet-induced skeletal muscle adaptations. However, the impact of diet on miRNA 
      expression during postexercise recovery remains unclear. The objective of this 
      study was to examine the effects of consuming carbohydrate or a nutrient-free 
      control on skeletal muscle miRNA expression during 3 h of recovery from aerobic 
      exercise. Using a randomized, crossover design, seven men (means +/- SD, age: 
      21 +/- 3 yr; body mass: 83 +/- 13 kg; V̇o(2peak): 43 +/- 2 mL/kg/min) completed 
      two-cycle ergometry glycogen depletion trials followed by 3 h of recovery while 
      consuming either carbohydrate (CHO: 1 g/kg/h) or control (CON: nutrient free). 
      Muscle biopsy samples were obtained under resting fasted conditions at baseline 
      and at the end of the 3-h recovery (REC) period. miRNA expression was determined 
      using unbiased RT-qPCR microarray analysis. Trials were separated by 7 days. 
      Twenty-five miRNAs were different (P < 0.05) between CHO and CON at REC, with 
      Let7i-5p and miR-195-5p being the most predictive of treatment. In vitro 
      overexpression of Let7i-5p and miR-195-p5 in C2C12 skeletal muscle cells 
      decreased (P < 0.05) the expression of protein breakdown (Foxo1, Trim63, Casp3, 
      and Atf4) genes, ubiquitylation, and protease enzyme activity compared with 
      control. Energy sensing (Prkaa1 and Prkab1) and glycolysis (Gsy1 and Gsk3b) genes 
      were lower (P < 0.05) with Let7i-5p overexpression compared with miR-195-5p and 
      control. Fat metabolism (Cpt1a, Scd1, and Hadha) genes were lower (P < 0.05) in 
      miR-195-5p than in control. These data indicate that consuming CHO after aerobic 
      exercise alters miRNA profiles compared with CON, and these differences may 
      govern mechanisms facilitating muscle recovery.NEW & NOTEWORTHY Results provide 
      novel insight into effects of carbohydrate intake on the expression of skeletal 
      muscle microRNA during early recovery from aerobic exercise and reveal that 
      Let7i-5p and miR-195-5p are important regulators of skeletal muscle protein 
      breakdown to aid in facilitating muscle recovery.
FAU - Margolis, Lee M
AU  - Margolis LM
AUID- ORCID: 0000-0002-0652-1304
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - Carrigan, Christopher T
AU  - Carrigan CT
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - Murphy, Nancy E
AU  - Murphy NE
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - DiBella, Marissa N
AU  - DiBella MN
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
AD  - Oak Ridge Institute of Science and Education, Belcamp, Maryland.
FAU - Wilson, Marques A
AU  - Wilson MA
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - Whitney, Claire C
AU  - Whitney CC
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - Howard, Emily E
AU  - Howard EE
AUID- ORCID: 0000-0001-7979-1236
AD  - Military Nutrition Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - Pasiakos, Stefan M
AU  - Pasiakos SM
AD  - Military Performance Division, U.S. Army Research Institute of Environmental 
      Medicine, Natick, Massachusetts.
FAU - Rivas, Donato A
AU  - Rivas DA
AUID- ORCID: 0000-0002-4500-6233
AD  - Nutrition, Exercise Physiology and Sarcopenia Laboratory, Jean Mayer USDA Human 
      Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts.
LA  - eng
GR  - K01 AG047247/AG/NIA NIH HHS/United States
GR  - P30 AG031679/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
DEP - 20220831
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Dietary Carbohydrates)
RN  - 9005-79-2 (Glycogen)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Humans
MH  - Male
MH  - Young Adult
MH  - Dietary Carbohydrates/pharmacology/metabolism
MH  - Exercise/physiology
MH  - *Glycogen/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Muscle, Skeletal/metabolism
PMC - PMC9639755
OTO - NOTNLM
OT  - Let7
OT  - endurance exercise
OT  - glycogen
OT  - miR-195
COIS- No conflicts of interest, financial or otherwise, are declared by the authors.
EDAT- 2022/09/01 06:00
MHDA- 2022/11/09 06:00
PMCR- 2023/11/01
CRDT- 2022/08/31 15:52
PHST- 2022/09/01 06:00 [pubmed]
PHST- 2022/11/09 06:00 [medline]
PHST- 2022/08/31 15:52 [entrez]
PHST- 2023/11/01 00:00 [pmc-release]
AID - E-00110-2022 [pii]
AID - 10.1152/ajpendo.00110.2022 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2022 Nov 1;323(5):E435-E447. doi: 
      10.1152/ajpendo.00110.2022. Epub 2022 Aug 31.