PMID- 36047990
OWN - NLM
STAT- MEDLINE
DCOM- 20220922
LR  - 20221207
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 56
IP  - 18
DP  - 2022 Sep 20
TI  - Selective Enrichment of Comammox Nitrospira in a Moving Bed Biofilm Reactor with 
      Sufficient Oxygen Supply.
PG  - 13338-13346
LID - 10.1021/acs.est.2c03299 [doi]
AB  - The recent discovery of comammox (complete ammonia oxidation) Nitrospira has 
      upended the long-held nitrification paradigm. Although comammox Nitrospira have 
      been identified in wastewater treatment systems, the conditions for their 
      dominance over canonical ammonia oxidizers remain unclear. Here, we report the 
      dominance of comammox Nitrospira in a moving bed biofilm reactor (MBBR) fed with 
      synthetic mainstream wastewater. Integrated 16S rRNA gene amplicon sequencing, 
      fluorescence in situ hybridization (FISH), and metagenomic sequencing methods 
      demonstrated the selective enrichment of comammox bacteria when the MBBR was 
      operated at a dissolved oxygen (DO) concentration above 6 mg O(2)/L. The 
      dominance of comammox Nitrospira over canonical ammonia oxidizers (i.e., 
      Nitrosomonas) was attributed to the low residual ammonium concentration 
      (0.02-0.52 mg N/L) formed in the high-DO MBBR. Two clade A comammox Nitrospira 
      were identified, which are phylogenetically close to Candidatus Nitrospira 
      nitrosa. Interestingly, cryosectioning-FISH showed these two comammox species 
      spatially distributed on the surface of the biofilm. Moreover, the 
      ammonia-oxidizing activity of comammox Nitrospira-dominated biofilms was 
      susceptible to the oxygen supply, which dropped by half with the DO concentration 
      decrease from 6 to 2 mg O(2)/L. These features collectively suggest a low 
      apparent oxygen affinity for the comammox Nitrospira-dominated biofilms in the 
      high-DO nitrifying MBBR.
FAU - Zhao, Jing
AU  - Zhao J
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St Lucia, Queensland 4072, Australia.
FAU - Zheng, Min
AU  - Zheng M
AUID- ORCID: 0000-0001-9148-7544
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St Lucia, Queensland 4072, Australia.
FAU - Su, Zicheng
AU  - Su Z
AUID- ORCID: 0000-0002-3189-6212
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St Lucia, Queensland 4072, Australia.
FAU - Liu, Tao
AU  - Liu T
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St Lucia, Queensland 4072, Australia.
FAU - Li, Jie
AU  - Li J
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St Lucia, Queensland 4072, Australia.
FAU - Guo, Jianhua
AU  - Guo J
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St Lucia, Queensland 4072, Australia.
FAU - Yuan, Zhiguo
AU  - Yuan Z
AUID- ORCID: 0000-0002-7566-1482
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St Lucia, Queensland 4072, Australia.
FAU - Hu, Shihu
AU  - Hu S
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St Lucia, Queensland 4072, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220901
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Ammonium Compounds)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Waste Water)
RN  - 7664-41-7 (Ammonia)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - *Ammonia
MH  - *Ammonium Compounds
MH  - Bacteria/genetics
MH  - Biofilms
MH  - Bioreactors
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrification
MH  - Oxidation-Reduction
MH  - Oxygen
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Wastewater/microbiology
OTO - NOTNLM
OT  - comammox Nitrospira
OT  - complete ammonia oxidation
OT  - metagenomics
OT  - moving bed biofilm reactor
OT  - oxygen affinity
EDAT- 2022/09/02 06:00
MHDA- 2022/09/23 06:00
CRDT- 2022/09/01 10:23
PHST- 2022/09/02 06:00 [pubmed]
PHST- 2022/09/23 06:00 [medline]
PHST- 2022/09/01 10:23 [entrez]
AID - 10.1021/acs.est.2c03299 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2022 Sep 20;56(18):13338-13346. doi: 
      10.1021/acs.est.2c03299. Epub 2022 Sep 1.