PMID- 36048577
OWN - NLM
STAT- MEDLINE
DCOM- 20221110
LR  - 20221110
IS  - 1365-2761 (Electronic)
IS  - 0140-7775 (Linking)
VI  - 45
IP  - 12
DP  - 2022 Dec
TI  - First identification of Nocardia seriolae GapA adhesion function and its three 
      B-cell epitopes with cell-binding activity.
PG  - 1845-1855
LID - 10.1111/jfd.13709 [doi]
AB  - Fish nocardiosis mainly caused by Nocardia seriolae (N. seriolae) is a serious 
      threat to aquaculture. Bacterial adhesion to host cells mediated by adhesin is an 
      initial step of pathogenesis. But it is not clear whether 
      glyceraldehyde-3-phosphate dehydrogenase (GapA) is an adhesin of N. seriolae. 
      Here, recombinant GapA protein (rGapA) was prokaryotic expressed, and its role in 
      the bacterial adhesion to Ctenopharyngodon idella kidney cells was investigated 
      by indirect immunofluorescence, protein-binding assay and adhesion inhibition 
      assay. The results showed that an obvious green fluorescence was observed on the 
      surface of the cells co-incubated with rGapA protein; the cytomembrane proteins 
      of the cells pretreated with rGapA could react with anti-rGapA antibody; and the 
      antibody significantly inhibited the adhesion ability of the bacteria. 
      Subsequently, B-cell linear epitopes of GapA protein were identified by using a 
      immunoinformatics approach combined with peptide ELISA and Western blot for the 
      first time. It was found that four predicted epitopes (Ep(58-69) , Ep(139-150) , 
      Ep(186-197) , Ep(318-329) ) could all react with anti-rGapA antibody and 
      obviously inhibit the immunoreactivity between rGapA and anti-rGapA antibody, and 
      they were confirmed as indeed B-cell linear epitopes of the protein. Furthermore, 
      flow cytometry analysis found the percentage of positive cells co-incubated with 
      FITC-labelled epitope peptides (Ep(139-150) , Ep(186-197) , Ep(318-329) ) was 
      significantly higher than those in the FITC-labelled Ep(58-69) , unrelated 
      control peptide and cell control. Collectively, GapA is an adhesin of N. 
      seriolae, and epitope peptides (Ep(139-150) , Ep(186-197) , Ep(318-329) ) possess 
      cell-binding activity, which are potential candidates for developing a multiple 
      epitopes-based adhesin vaccine against fish nocardiosis.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Guo, Jiajing
AU  - Guo J
AD  - Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of 
      Animal Science and Technology, Anhui Agricultural University, Hefei, P. R. China.
FAU - Yue, Xiaozhen
AU  - Yue X
AD  - Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of 
      Animal Science and Technology, Anhui Agricultural University, Hefei, P. R. China.
FAU - Chang, Jiaojiao
AU  - Chang J
AD  - Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of 
      Animal Science and Technology, Anhui Agricultural University, Hefei, P. R. China.
FAU - Zhang, Zhenyuan
AU  - Zhang Z
AD  - Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of 
      Animal Science and Technology, Anhui Agricultural University, Hefei, P. R. China.
FAU - Li, Jinnian
AU  - Li J
AD  - Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of 
      Animal Science and Technology, Anhui Agricultural University, Hefei, P. R. China.
FAU - Liu, Xuelan
AU  - Liu X
AD  - Anhui Province Key Lab of Veterinary Pathobiology and Disease Control, College of 
      Animal Science and Technology, Anhui Agricultural University, Hefei, P. R. China.
LA  - eng
GR  - China Natural Science Foundation, Project No.31672698/
GR  - Graduate Innovation Foundation, Project No. 2021yjs-18/
GR  - Training Program for College Students' Innovation in Anhui Agricultural 
      University, Project No. XJDC2021450./
GR  - Anhui Agricultural University/
PT  - Journal Article
DEP - 20220901
PL  - England
TA  - J Fish Dis
JT  - Journal of fish diseases
JID - 9881188
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
RN  - 0 (Recombinant Proteins)
RN  - Nocardia seriolae
SB  - IM
MH  - Animals
MH  - Epitopes, B-Lymphocyte
MH  - Fluorescein-5-isothiocyanate
MH  - *Fish Diseases/microbiology
MH  - Phylogeny
MH  - *Nocardia/physiology
MH  - *Nocardia Infections/microbiology
MH  - *Carps
MH  - Recombinant Proteins
OTO - NOTNLM
OT  - Nocardia seriolae
OT  - B-cell linear epitope
OT  - GapA protein
OT  - adhesin
OT  - cell-binding activity
EDAT- 2022/09/02 06:00
MHDA- 2022/11/11 06:00
CRDT- 2022/09/01 12:23
PHST- 2022/08/11 00:00 [revised]
PHST- 2022/06/28 00:00 [received]
PHST- 2022/08/16 00:00 [accepted]
PHST- 2022/09/02 06:00 [pubmed]
PHST- 2022/11/11 06:00 [medline]
PHST- 2022/09/01 12:23 [entrez]
AID - 10.1111/jfd.13709 [doi]
PST - ppublish
SO  - J Fish Dis. 2022 Dec;45(12):1845-1855. doi: 10.1111/jfd.13709. Epub 2022 Sep 1.