PMID- 36051397
OWN - NLM
STAT- MEDLINE
DCOM- 20220908
LR  - 20220914
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 13
DP  - 2022
TI  - Signaling pathways activated by sea bass gonadotropin-inhibitory hormone peptides 
      in COS-7 cells transfected with their cognate receptor.
PG  - 982246
LID - 10.3389/fendo.2022.982246 [doi]
LID - 982246
AB  - Results of previous studies provided evidence for the existence of a functional 
      gonadotropin-inhibitory hormone (GnIH) system in the European sea bass, 
      Dicentrarchus labrax, which exerted an inhibitory action on the 
      brain-pituitary-gonadal axis of this species. Herein, we further elucidated the 
      intracellular signaling pathways mediating in sea bass GnIH actions and the 
      potential interactions with sea bass kisspeptin (Kiss) signaling. Although GnIH1 
      and GnIH2 had no effect on basal CRE-luc activity, they significantly decreased 
      forskolin-elicited CRE-luc activity in COS-7 cells transfected with their cognate 
      receptor GnIHR. Moreover, an evident increase in SRE-luc activity was noticed 
      when COS-7 cells expressing GnIHR were challenged with both GnIH peptides, and 
      this stimulatory action was significantly reduced by two inhibitors of the PKC 
      pathway. Notably, GnIH2 antagonized Kiss2-evoked CRE-luc activity in COS-7 cells 
      expressing GnIHR and Kiss2 receptor (Kiss2R). However, GnIH peptides did not 
      alter NFAT-RE-luc activity and ERK phosphorylation levels. These data indicate 
      that sea bass GnIHR signals can be transduced through the PKA and PKC pathways, 
      and GnIH can interfere with kisspeptin actions by reducing its signaling. Our 
      results provide additional evidence for the understanding of signaling pathways 
      activated by GnIH peptides in teleosts, and represent a starting point for the 
      study of interactions with multiple neuroendocrine factors on cell signaling.
CI  - Copyright (c) 2022 Wang, Paullada-Salmeron, Verges-Castillo, Gomez and Munoz-Cueto.
FAU - Wang, Bin
AU  - Wang B
AD  - Department of Biology, Faculty of Marine and Environmental Sciences, University 
      of Cadiz, Cadiz, Spain.
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Qingdao, China.
AD  - Laboratory for Marine Fisheries and Food Production Processes, Pilot National 
      Laboratory for Marine Science and Technology (Qingdao), Qingdao, China.
FAU - Paullada-Salmeron, Jose A
AU  - Paullada-Salmeron JA
AD  - Department of Biology, Faculty of Marine and Environmental Sciences, University 
      of Cadiz, Cadiz, Spain.
AD  - Marine Research Institute (INMAR), Marine Campus of International Excellence 
      (CEIMAR) and Agrifood Campus of International Excellence (ceiA3), Cadiz, Spain.
AD  - The European University of the Seas (SEA-EU), Cadiz, Spain.
FAU - Verges-Castillo, Alba
AU  - Verges-Castillo A
AD  - Department of Biology, Faculty of Marine and Environmental Sciences, University 
      of Cadiz, Cadiz, Spain.
AD  - Marine Research Institute (INMAR), Marine Campus of International Excellence 
      (CEIMAR) and Agrifood Campus of International Excellence (ceiA3), Cadiz, Spain.
AD  - The European University of the Seas (SEA-EU), Cadiz, Spain.
FAU - Gomez, Ana
AU  - Gomez A
AD  - Institute of Aquaculture of Torre de la Sal, CSIC, Castellon, Spain.
FAU - Munoz-Cueto, Jose A
AU  - Munoz-Cueto JA
AD  - Department of Biology, Faculty of Marine and Environmental Sciences, University 
      of Cadiz, Cadiz, Spain.
AD  - Marine Research Institute (INMAR), Marine Campus of International Excellence 
      (CEIMAR) and Agrifood Campus of International Excellence (ceiA3), Cadiz, Spain.
AD  - The European University of the Seas (SEA-EU), Cadiz, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220816
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Kisspeptins)
SB  - IM
MH  - Animals
MH  - *Bass/physiology
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Chorionic Gonadotropin
MH  - Kisspeptins/metabolism
MH  - Signal Transduction
PMC - PMC9424679
OTO - NOTNLM
OT  - GnIH
OT  - GnIH receptor
OT  - kisspeptin
OT  - kisspeptin receptor
OT  - signaling pathway
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/09/03 06:00
MHDA- 2022/09/09 06:00
PMCR- 2022/01/01
CRDT- 2022/09/02 02:30
PHST- 2022/06/30 00:00 [received]
PHST- 2022/07/22 00:00 [accepted]
PHST- 2022/09/02 02:30 [entrez]
PHST- 2022/09/03 06:00 [pubmed]
PHST- 2022/09/09 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2022.982246 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2022 Aug 16;13:982246. doi: 
      10.3389/fendo.2022.982246. eCollection 2022.