PMID- 36054755
OWN - NLM
STAT- MEDLINE
DCOM- 20220922
LR  - 20221017
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 94
IP  - 37
DP  - 2022 Sep 20
TI  - Colorimetric Cotton Swab for Viral Protease Detection.
PG  - 12699-12705
LID - 10.1021/acs.analchem.2c02033 [doi]
AB  - Reporting the activity of a specific viral protease remains an acute need for 
      rapid point-of-care detection strategies that can distinguish active infection 
      from a resolved infection. In this work, we present a simple colorimetric 
      approach for reporting the activity of a specific viral protease through direct 
      color conversion on a cotton swab, which has the potential to be extended to 
      detect the corresponding virus. We use SARS-CoV-2 viral protease as a 
      proof-of-concept model system. We use 4-aminomalachite green (4-AMG) as the base 
      chromophore structure to design a CoV2-AMG reporter, which is selective toward 
      the SARS-CoV-2 M(pro) but does not produce any observable color change in the 
      presence of other viral proteases. The color change is observable by the naked 
      eye, as well as smartphone imaging, which affords a lower limit of detection. The 
      simplicity and generalizability of the method could be instrumental in combating 
      future viral outbreaks.
FAU - Dutta, Ranit
AU  - Dutta R
FAU - Makhaik, Sparsh
AU  - Makhaik S
FAU - Zhao, Peiyao
AU  - Zhao P
FAU - Cruz, Kristalle G
AU  - Cruz KG
FAU - Park, Kwang-Won
AU  - Park KW
AUID- ORCID: 0000-0002-2700-7005
FAU - Liu, Hongxu
AU  - Liu H
AUID- ORCID: 0000-0003-0076-1259
FAU - Andrew, Trisha L
AU  - Andrew TL
AUID- ORCID: 0000-0002-8193-2912
FAU - Hardy, Jeanne A
AU  - Hardy JA
AUID- ORCID: 0000-0002-3406-7997
FAU - Thayumanavan, S
AU  - Thayumanavan S
AUID- ORCID: 0000-0002-6475-6726
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20220902
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.- (Viral Proteases)
SB  - IM
MH  - *COVID-19/diagnosis
MH  - Colorimetry/methods
MH  - Humans
MH  - Peptide Hydrolases
MH  - *SARS-CoV-2
MH  - Viral Proteases
EDAT- 2022/09/03 06:00
MHDA- 2022/09/23 06:00
CRDT- 2022/09/02 15:13
PHST- 2022/09/03 06:00 [pubmed]
PHST- 2022/09/23 06:00 [medline]
PHST- 2022/09/02 15:13 [entrez]
AID - 10.1021/acs.analchem.2c02033 [doi]
PST - ppublish
SO  - Anal Chem. 2022 Sep 20;94(37):12699-12705. doi: 10.1021/acs.analchem.2c02033. 
      Epub 2022 Sep 2.