PMID- 36057923
OWN - NLM
STAT- MEDLINE
DCOM- 20221206
LR  - 20221206
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 103
IP  - 2
DP  - 2023 Jan 30
TI  - In silico tools to assess the potential allergenicity of shiitake mushrooms 
      (Lentinula edodes).
PG  - 877-890
LID - 10.1002/jsfa.12199 [doi]
AB  - BACKGROUND: Computational tools may have an edge over conventional methods for 
      the preliminary evaluation of food allergenicity. In this study, the allergenic 
      potential of Lentinula edodes was evaluated and validated using in silico tools. 
      RESULTS: The potential cross-reactivity of mushroom proteins with fungal 
      allergens was determined using sequence alignment - the Fast Alignment (FASTA) 
      and Basic Local Alignment Search Tool (BLAST) algorithm. Eight L. edodes proteins 
      were cross-reactive with allergens from fungal origin, showing 52%-89% sequence 
      identity using FASTA algorithm-based alignment. The BLAST data were corroborated 
      by percentage identity and query coverage. Physico-chemical property-based 
      allergenicity was deciphered by AlgPred, Allermatch, and AllergenFP software, 
      which predicted six out of eight proteins as potential allergens. Sequence 
      alignment showed 66%-86% conservancy between mushroom protein and known fungal 
      allergens. Secondary structure and amino acid composition supported structural 
      affinity between query and fungal proteins. Three-dimensional structures of five 
      mushroom proteins were generated, quality assessed, and superimposed with fungal 
      allergens, suggesting possible allergenicity of mushroom proteins. An 
      enzyme-linked immunosorbent assay (ELISA) demonstrated immunoglobulin E (IgE) 
      binding in 13 out of 21 food-hypersensitive patients' sera. CONCLUSION: In silico 
      tools provide preliminary indications about the potential allergenicity and 
      cross-reactivity of mushroom proteins. This approach may be used for the 
      prelusive allergenicity assessment of allergen sources. (c) 2022 Society of 
      Chemical Industry.
CI  - (c) 2022 Society of Chemical Industry.
FAU - Vashisht, Srishti
AU  - Vashisht S
AD  - Allergy and Immunology Section, CSIR-Institute of Genomicsand Integrative 
      Biology, Delhi, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
FAU - Singh, Naresh
AU  - Singh N
AD  - Allergy and Immunology Section, CSIR-Institute of Genomicsand Integrative 
      Biology, Delhi, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
FAU - Sharma, Akansha
AU  - Sharma A
AD  - Allergy and Immunology Section, CSIR-Institute of Genomicsand Integrative 
      Biology, Delhi, India.
FAU - Saini, Neeru
AU  - Saini N
AD  - Allergy and Immunology Section, CSIR-Institute of Genomicsand Integrative 
      Biology, Delhi, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
FAU - Gaur, Shailendra Nath
AU  - Gaur SN
AD  - Department of Respiratory Medicine, Vallabhbhai Patel Chest Institute, University 
      of Delhi, Delhi, India.
FAU - Arora, Naveen
AU  - Arora N
AUID- ORCID: 0000-0001-5115-1034
AD  - Allergy and Immunology Section, CSIR-Institute of Genomicsand Integrative 
      Biology, Delhi, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
LA  - eng
GR  - HCP0019/Council of Scientific and Industrial Research, India/
PT  - Journal Article
DEP - 20220920
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Allergens)
SB  - IM
MH  - Humans
MH  - Allergens/chemistry
MH  - *Shiitake Mushrooms
MH  - *Food Hypersensitivity
MH  - Sequence Alignment
MH  - Cross Reactions
OTO - NOTNLM
OT  - IgE based allergenicity assessment
OT  - allergenicity assessment
OT  - food allergy
OT  - in silico allergenicity assessment
OT  - mushroom allergy
EDAT- 2022/09/05 06:00
MHDA- 2022/12/07 06:00
CRDT- 2022/09/04 13:57
PHST- 2022/08/05 00:00 [revised]
PHST- 2021/12/27 00:00 [received]
PHST- 2022/09/04 00:00 [accepted]
PHST- 2022/09/05 06:00 [pubmed]
PHST- 2022/12/07 06:00 [medline]
PHST- 2022/09/04 13:57 [entrez]
AID - 10.1002/jsfa.12199 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2023 Jan 30;103(2):877-890. doi: 10.1002/jsfa.12199. Epub 2022 
      Sep 20.