PMID- 36059443 OWN - NLM STAT- MEDLINE DCOM- 20220908 LR - 20220914 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 13 DP - 2022 TI - Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas. PG - 928628 LID - 10.3389/fimmu.2022.928628 [doi] LID - 928628 AB - The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing of before and after-mortality larval samples as well as dead and surviving adults from a viral challenge. Analysis of the resequencing data identified 5,271 SNPs and 1,883 genomic regions covering 3,111 genes in larvae, and 18,692 SNPs and 28,314 regions covering 4,863 genes in adults that were significantly associated with herpesvirus-caused mortalities. Only 1,653 of the implicated genes were shared by larvae and adults, suggesting that the antiviral response or resistance in larvae and adults involves different sets of genes or differentiated members of expanded gene families. Combined analyses with previous transcriptomic data from challenge experiments revealed that transcription of many mortality-associated genes was also significantly upregulated by herpesvirus infection confirming their importance in antiviral response. Key immune response genes especially those encoding antiviral receptors such as TLRs and RLRs displayed strong association between variation in regulatory region and herpesvirus-caused mortality, suggesting they may confer resistance through transcriptional modulation. These results point to previously undescribed genetic mechanisms for disease resistance at different developmental stages and provide candidate polymorphisms and genes that are valuable for understanding antiviral immune responses and breeding for herpesvirus resistance. CI - Copyright (c) 2022 Yao, Li, Guan, He, Jouaux, Xu, Guo, Zhang and Zhang. FAU - Yao, Shanshan AU - Yao S AD - Chinese Academy of Sciences (CAS) and Shandong Province Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. AD - Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. AD - College of Life Sciences, Qingdao University, Qingdao, China. FAU - Li, Li AU - Li L AD - Chinese Academy of Sciences (CAS) and Shandong Province Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. AD - Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. AD - University of Chinese Academy of Sciences, College of Marine Science, Beijing, China. FAU - Guan, Xudong AU - Guan X AD - Chinese Academy of Sciences (CAS) and Shandong Province Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. FAU - He, Yan AU - He Y AD - Ministry of Education (MOE) Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China. FAU - Jouaux, Aude AU - Jouaux A AD - UMR BOREA, "Biologie des Organismes et Ecosystemes Aquatiques", MNHN, UPMC, UCBN, CNRS-7208, IRD, Universite de Caen Basse-Normandie, Esplanade de la Paix, Caen, France. FAU - Xu, Fei AU - Xu F AD - Chinese Academy of Sciences (CAS) and Shandong Province Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. FAU - Guo, Ximing AU - Guo X AD - Haskin Shellfish Research Laboratory, Department of Marine and Coastal Sciences, Rutgers University, Port Norris, NJ, United States. FAU - Zhang, Guofan AU - Zhang G AD - Chinese Academy of Sciences (CAS) and Shandong Province Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. AD - Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. AD - University of Chinese Academy of Sciences, College of Marine Science, Beijing, China. FAU - Zhang, Linlin AU - Zhang L AD - Chinese Academy of Sciences (CAS) and Shandong Province Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China. AD - Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. AD - University of Chinese Academy of Sciences, College of Marine Science, Beijing, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220819 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Antiviral Agents) RN - Ostreid herpesvirus 1 SB - IM MH - Animals MH - Antiviral Agents MH - *Crassostrea MH - DNA Viruses MH - Genomics MH - *Herpesviridae/genetics MH - Larva/genetics PMC - PMC9437489 OTO - NOTNLM OT - Ostreid herpesvirus OT - antiviral innate immunity OT - disease resistance OT - larval mortality OT - molluscan aquaculture OT - oyster OT - pooled-resequencing OT - transcriptomic response COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/09/06 06:00 MHDA- 2022/09/09 06:00 PMCR- 2022/01/01 CRDT- 2022/09/05 03:37 PHST- 2022/04/26 00:00 [received] PHST- 2022/07/29 00:00 [accepted] PHST- 2022/09/05 03:37 [entrez] PHST- 2022/09/06 06:00 [pubmed] PHST- 2022/09/09 06:00 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2022.928628 [doi] PST - epublish SO - Front Immunol. 2022 Aug 19;13:928628. doi: 10.3389/fimmu.2022.928628. eCollection 2022.