PMID- 36059519 OWN - NLM STAT- MEDLINE DCOM- 20220908 LR - 20220914 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 13 DP - 2022 TI - Human dendritic cell targeting peptide can be targeted to porcine dendritic cells to improve antigen capture efficiency to stimulate stronger immune response. PG - 950597 LID - 10.3389/fimmu.2022.950597 [doi] LID - 950597 AB - Dendritic cells (DCs) play a key role in the natural recognition of pathogens and subsequent activation of adaptive immune responses due to their potent antigen-presenting ability. Dendritic cell-targeting peptide (DCpep) is strongly targeted to DCs, which often express antigens, to enhance the efficacy of vaccines. Our previous study showed that recombinant Lactobacillus expressing human DCpep could significantly induce stronger immune responses than recombinant Lactobacillus without DCpep, but the mechanism remains unclear. In this study, the mechanism by which DCpep enhances the immune response against recombinant Lactobacillus was explored. Fluorescence-labeled human DCpep was synthesized to evaluate the binding ability of human DCpep to porcine monocyte-derived dendritic cells (Mo-DCs) and DCs of the small intestine. The effects of Mo-DC function induced by recombinant Lactobacillus expressing human DCpep fused with the porcine epidemic diarrhea virus (PEDV) core neutralizing epitope (COE) antigen were also investigated. The results showed that human DCpep bind to porcine DCs, but not to porcine small intestinal epithelial cells. Human DCpep can also improve the capture efficiency of recombinant Lactobacillus by Mo-DCs, promote the maturation of dendritic cells, secrete more cytokines, and enhance the ability of porcine DCs to activate T-cell proliferation. Taken together, these results promote advanced understanding of the mechanism by which DCpep enhances immune responses. We found that some DCpeps are conserved between humans and pigs, which provides a theoretical basis for the development of a DC-targeted vaccine. CI - Copyright (c) 2022 Xia, Yang, Guo, Guo, Xin, Jiang, Cui, Zhou, Qiao, Wang, Li, Shan, Tang, Wang and Li. FAU - Xia, Tian AU - Xia T AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. FAU - Yang, Huizhu AU - Yang H AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. FAU - Guo, Yuyao AU - Guo Y AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. FAU - Guo, Tiantian AU - Guo T AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. FAU - Xin, Lingxiang AU - Xin L AD - Division of Bacterial Biologics Testing (I) China Institute of Veterinary Drug Control (IVDC), Beijing, China. FAU - Jiang, Yanping AU - Jiang Y AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Cui, Wen AU - Cui W AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Zhou, Han AU - Zhou H AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Qiao, Xinyuan AU - Qiao X AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Wang, Xiaona AU - Wang X AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Li, Jiaxuan AU - Li J AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Shan, Zhifu AU - Shan Z AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Tang, Lijie AU - Tang L AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Wang, Li AU - Wang L AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. FAU - Li, Yijing AU - Li Y AD - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China. AD - China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeastern Science Inspection Station, Harbin, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220819 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Peptides) RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Dendritic Cells MH - Humans MH - Lactobacillus MH - Lymphocyte Activation MH - Peptides MH - *Porcine epidemic diarrhea virus MH - Swine MH - *Viral Vaccines PMC - PMC9437479 OTO - NOTNLM OT - antigen-presenting OT - human dendritic cells-targeting peptide OT - porcine dendritic cells OT - porcine epidemic diarrhea virus OT - probiotic bacteria COIS- The research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/09/06 06:00 MHDA- 2022/09/09 06:00 PMCR- 2022/01/01 CRDT- 2022/09/05 03:38 PHST- 2022/05/23 00:00 [received] PHST- 2022/07/22 00:00 [accepted] PHST- 2022/09/05 03:38 [entrez] PHST- 2022/09/06 06:00 [pubmed] PHST- 2022/09/09 06:00 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2022.950597 [doi] PST - epublish SO - Front Immunol. 2022 Aug 19;13:950597. doi: 10.3389/fimmu.2022.950597. eCollection 2022.