PMID- 36059705
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230412
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 12
DP  - 2022
TI  - High expression of nectin-1 indicates a poor prognosis and promotes metastasis in 
      hepatocellular carcinoma.
PG  - 953529
LID - 10.3389/fonc.2022.953529 [doi]
LID - 953529
AB  - OBJECTIVES: Nectins are a new class of cell-adhesion molecules that play an 
      important role in tumorigenesis and disease progression. The aim of this study 
      was to investigate the prognostic and pathogenetic roles of nectins in 
      hepatocellular carcinoma (HCC). METHODS: The expression levels of the nectin 
      family in HCC and their role in prognosis were analyzed by bioinformatics 
      analysis based on The Cancer Genome Atlas (TCGA) liver hepatocellular carcinoma 
      database. The correlations between nectins and immune cells were analyzed using 
      TIMER. The functional enrichment of the nectin-1 coexpression network was 
      evaluated in TCGA cohort, and the expression levels of nectin-1 were detected by 
      immunohistochemistry and Western blot analysis. A Transwell kit was used for cell 
      migration experiments. Cell proliferation was analyzed using Cell Counting Kit-8. 
      RESULTS: The expression levels of nectin-1 protein in the cancer tissues of 28 
      patients with HCC were higher than those in paracancerous tissues. The 
      Kaplan-Meier plotter analysis showed that the high expression of all nectin 
      family numbers was related to the poor prognosis of HCC patients. The abnormal 
      expression of nectin-1 effectively distinguished the prognosis at different 
      stages and grades of HCC. The high expression of 17 methylation sites of the 
      nectin-1 gene was related to the high overall survival of HCC patients. Kyoto 
      Encyclopedia of Genes and Genomes analysis of genes negatively correlated with 
      nectin-1, revealing their close relation to the regulation of the immune-effector 
      process. Pearson's correlation analysis showed that nectin-1 was significantly 
      positively correlated with multiple immune genes and B cells, CD4(+) T cells, 
      macrophages, neutrophils, and dendritic cell infiltration. Cell proliferation of 
      the knockdown (KD) group decreased significantly compared to the NC-KD group. The 
      number of metastatic cells in the KD group decreased significantly compared to 
      that in the NC-KD group. CONCLUSIONS: Abnormal expression of nectins and multiple 
      methylation sites closely correlates with poor prognosis in HCC patients. Nectins 
      are related to immune cell infiltration and immune-related genes. In particular, 
      nectin-1 can promote the proliferation and migration of liver cancer cells and 
      distinguish the prognosis at different stages and grades of HCC. Nectin-1 might 
      be a new potential molecular marker for prognostic evaluation and also a 
      therapeutic target for HCC.
CI  - Copyright (c) 2022 Wang, Xing, Chen, Yang, Wang and Xing.
FAU - Wang, Xuequan
AU  - Wang X
AD  - Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, 
      Linhai, China.
FAU - Xing, Ziming
AU  - Xing Z
AD  - Academy of Medical Engineering and Translational Medicine, Tianjin University, 
      Tianjin, China.
FAU - Chen, Huazhong
AU  - Chen H
AD  - Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, 
      Linhai, China.
FAU - Yang, Haihua
AU  - Yang H
AD  - Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, 
      Linhai, China.
FAU - Wang, Qiupeng
AU  - Wang Q
AD  - Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, 
      Linhai, China.
FAU - Xing, Tongjing
AU  - Xing T
AD  - Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, 
      Linhai, China.
LA  - eng
PT  - Journal Article
DEP - 20220818
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
EIN - Front Oncol. 2023 Mar 21;13:1134139. PMID: 37025598
PMC - PMC9433868
OTO - NOTNLM
OT  - cell proliferation
OT  - hepatocellular carcinoma
OT  - metastasis
OT  - nectin-1
OT  - prognostic
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/09/06 06:00
MHDA- 2022/09/06 06:01
PMCR- 2022/01/01
CRDT- 2022/09/05 03:41
PHST- 2022/05/26 00:00 [received]
PHST- 2022/07/22 00:00 [accepted]
PHST- 2022/09/05 03:41 [entrez]
PHST- 2022/09/06 06:00 [pubmed]
PHST- 2022/09/06 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2022.953529 [doi]
PST - epublish
SO  - Front Oncol. 2022 Aug 18;12:953529. doi: 10.3389/fonc.2022.953529. eCollection 
      2022.