PMID- 36060961
OWN - NLM
STAT- MEDLINE
DCOM- 20220908
LR  - 20220913
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 13
DP  - 2022
TI  - Distinct impacts of fat and fructose on the liver, muscle, and adipose tissue 
      metabolome: An integrated view.
PG  - 898471
LID - 10.3389/fendo.2022.898471 [doi]
LID - 898471
AB  - OBJECTIVE: In the last years, changes in dietary habits have contributed to the 
      increasing prevalence of metabolic disorders, such as non-alcoholic fatty liver 
      disease (NAFLD) and type 2 diabetes mellitus (T2DM). The differential burden of 
      lipids and fructose on distinct organs needs to be unveiled. Herein, we 
      hypothesized that high-fat and high-fructose diets differentially affect the 
      metabolome of insulin-sensitive organs such as the liver, muscle, and different 
      adipose tissue depots. METHODS: We have studied the impact of 12 weeks of a 
      control (11.50% calories from fat, 26.93% from protein, and 61.57% from 
      carbohydrates), high-fat/sucrose (HFat), or high-fructose (HFruct) feeding on 
      C57Bl/6J male mice. Besides glucose homeostasis, we analyzed the hepatic levels 
      of glucose and lipid-metabolism-related genes and the metabolome of the liver, 
      the muscle, and white (WAT) and brown adipose tissue (BAT) depots. RESULTS: HFat 
      diet led to a more profound impact on hepatic glucose and lipid metabolism than 
      HFruct, with mice presenting glucose intolerance, increased saturated fatty 
      acids, and no glycogen pool, yet both HFat and HFruct presented hepatic insulin 
      resistance. HFat diet promoted a decrease in glucose and lactate pools in the 
      muscle and an increase in glutamate levels. While HFat had alterations in BAT 
      metabolites that indicate increased thermogenesis, HFruct led to an increase in 
      betaine, a protective metabolite against fructose-induced inflammation. 
      CONCLUSIONS: Our data illustrate that HFat and HFruct have a negative but 
      distinct impact on the metabolome of the liver, muscle, WAT, and BAT.
CI  - Copyright (c) 2022 Meneses, Sousa-Lima, Jarak, Raposo, Alves and Macedo.
FAU - Meneses, Maria Joao
AU  - Meneses MJ
AD  - iNOVA4Health, NOVA Medical School/Faculdade de Ciencias Medicas (NMS/FCM), 
      Universidade Nova de Lisboa, Lisbon, Portugal.
AD  - Portuguese Diabetes Association - Education and Research Center (APDP-ERC), 
      Lisbon, Portugal.
FAU - Sousa-Lima, Ines
AU  - Sousa-Lima I
AD  - iNOVA4Health, NOVA Medical School/Faculdade de Ciencias Medicas (NMS/FCM), 
      Universidade Nova de Lisboa, Lisbon, Portugal.
FAU - Jarak, Ivana
AU  - Jarak I
AD  - Department of Pharmaceutical Technology, Faculty of Pharmacy, University of 
      Coimbra, Coimbra, Portugal.
AD  - Department of Anatomy and Unit for Multidisciplinary Research in Biomedicine 
      (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of 
      Porto, Porto, Portugal.
FAU - Raposo, Joao F
AU  - Raposo JF
AD  - iNOVA4Health, NOVA Medical School/Faculdade de Ciencias Medicas (NMS/FCM), 
      Universidade Nova de Lisboa, Lisbon, Portugal.
AD  - Portuguese Diabetes Association - Education and Research Center (APDP-ERC), 
      Lisbon, Portugal.
FAU - Alves, Marco G
AU  - Alves MG
AD  - Department of Anatomy and Unit for Multidisciplinary Research in Biomedicine 
      (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of 
      Porto, Porto, Portugal.
FAU - Macedo, Maria Paula
AU  - Macedo MP
AD  - iNOVA4Health, NOVA Medical School/Faculdade de Ciencias Medicas (NMS/FCM), 
      Universidade Nova de Lisboa, Lisbon, Portugal.
AD  - Portuguese Diabetes Association - Education and Research Center (APDP-ERC), 
      Lisbon, Portugal.
AD  - Medical Sciences Department, University of Aveiro, Aveiro, Portugal.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220817
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
RN  - 30237-26-4 (Fructose)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adipose Tissue, Brown/metabolism
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - *Fructose/adverse effects
MH  - Glucose/metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Metabolome
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscles
PMC - PMC9428722
OTO - NOTNLM
OT  - adipose tissue
OT  - diet
OT  - metabolomics
OT  - muscle
OT  - non-alcoholic fatty liver disease
OT  - prediabetes
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest. The reviewer RS declared a shared affiliation with the 
      author MA to the handling editor at the time of review.
EDAT- 2022/09/06 06:00
MHDA- 2022/09/09 06:00
PMCR- 2022/01/01
CRDT- 2022/09/05 04:01
PHST- 2022/03/17 00:00 [received]
PHST- 2022/07/12 00:00 [accepted]
PHST- 2022/09/05 04:01 [entrez]
PHST- 2022/09/06 06:00 [pubmed]
PHST- 2022/09/09 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2022.898471 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2022 Aug 17;13:898471. doi: 
      10.3389/fendo.2022.898471. eCollection 2022.