PMID- 36064647
OWN - NLM
STAT- MEDLINE
DCOM- 20220908
LR  - 20220922
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 13
IP  - 1
DP  - 2022 Sep 5
TI  - Systemic proteomics and miRNA profile analysis of exosomes derived from human 
      pluripotent stem cells.
PG  - 449
LID - 10.1186/s13287-022-03142-1 [doi]
LID - 449
AB  - BACKGROUND: Increasing studies have reported the therapeutic effect of 
      mesenchymal stem cell (MSC)-derived exosomes by which protein and miRNA are 
      clearly characterized. However, the proteomics and miRNA profiles of exosomes 
      derived from human embryonic stem cells (hESCs) and human-induced pluripotent 
      stem cells (hiPSCs) remain unclear. METHODS: In this study, we isolated exosomes 
      from hESCs, hiPSCs, and human umbilical cord mesenchymal stem cells (hUC-MSCs) 
      via classic ultracentrifugation and a 0.22-mum filter, followed by the 
      conservative identification. Tandem mass tag labeling and label-free relative 
      peptide quantification together defined their proteomics. High-throughput 
      sequencing was performed to determine miRNA profiles. Then, we conducted a 
      bioinformatics analysis to identify the dominant biological processes and 
      pathways modulated by exosome cargos. Finally, the western blot and RT-qPCR were 
      performed to detect the actual loads of proteins and miRNAs in three types of 
      exosomes. RESULTS: Based on our study, the cargos from three types of exosomes 
      contribute to sophisticated biological processes. In comparison, hESC exosomes 
      (hESC-Exos) were superior in regulating development, metabolism, and anti-aging, 
      and hiPSC exosomes (hiPSC-Exos) had similar biological functions as hESC-Exos, 
      whereas hUC-MSCs exosomes (hUC-MSC-Exos) contributed more to immune regulation. 
      CONCLUSIONS: The data presented in our study help define the protein and miRNA 
      landscapes of three exosomes, predict their biological functions via systematic 
      and comprehensive network analysis at the system level, and reveal their 
      respective potential applications in different fields so as to optimize exosome 
      selection in preclinical and clinical trials.
CI  - (c) 2022. The Author(s).
FAU - Bi, Youkun
AU  - Bi Y
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Qiao, Xinlong
AU  - Qiao X
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Liu, Qun
AU  - Liu Q
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Song, Shaole
AU  - Song S
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Zhu, Keqi
AU  - Zhu K
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Qiu, Xun
AU  - Qiu X
AD  - Department of Medical Oncology, The Second Affiliated Hospital of Dalian Medical 
      University, Dalian, 116023, China.
FAU - Zhang, Xiang
AU  - Zhang X
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
FAU - Jia, Ce
AU  - Jia C
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
FAU - Wang, Huiwen
AU  - Wang H
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
FAU - Yang, Zhiguang
AU  - Yang Z
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Sixth Department of Liver Disease, Dalian Public Health Clinical Center, Dalian 
      Medical University, Dalian, 116023, China. zy730302@aliyun.com.
FAU - Ji, Guangju
AU  - Ji G
AUID- ORCID: 0000-0001-8626-3490
AD  - Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese 
      Academy of Sciences, Beijing, 100101, China. gj28@ibp.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220905
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (MicroRNAs)
SB  - IM
MH  - *Exosomes/genetics/metabolism
MH  - Humans
MH  - *Induced Pluripotent Stem Cells/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Proteomics
MH  - Umbilical Cord
PMC - PMC9444124
OTO - NOTNLM
OT  - Exosomes
OT  - Human embryonic stem cells
OT  - Human umbilical cord mesenchymal stem cells
OT  - Human-induced pluripotent stem cells
OT  - Proteomics
OT  - miRNA
COIS- The authors declare no potential conflict of interest. The authors declare no 
      potential conflict of interest.
EDAT- 2022/09/06 06:00
MHDA- 2022/09/09 06:00
PMCR- 2022/09/05
CRDT- 2022/09/05 23:54
PHST- 2022/05/23 00:00 [received]
PHST- 2022/08/16 00:00 [accepted]
PHST- 2022/09/05 23:54 [entrez]
PHST- 2022/09/06 06:00 [pubmed]
PHST- 2022/09/09 06:00 [medline]
PHST- 2022/09/05 00:00 [pmc-release]
AID - 10.1186/s13287-022-03142-1 [pii]
AID - 3142 [pii]
AID - 10.1186/s13287-022-03142-1 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2022 Sep 5;13(1):449. doi: 10.1186/s13287-022-03142-1.