PMID- 36064726
OWN - NLM
STAT- MEDLINE
DCOM- 20220907
LR  - 20240102
IS  - 1751-0147 (Electronic)
IS  - 0044-605X (Print)
IS  - 0044-605X (Linking)
VI  - 64
IP  - 1
DP  - 2022 Sep 5
TI  - Analytical performance of a canine ELISA monocyte chemoattractant protein-1 assay 
      for use in cats and evaluation of circulating levels in normal weight and obese 
      cats.
PG  - 22
LID - 10.1186/s13028-022-00640-3 [doi]
LID - 22
AB  - BACKGROUND: In human and murine obesity, adipose tissue dwelling macrophages and 
      adipocytes produce monocyte chemoattractant protein-1 (MCP-1) leading to systemic 
      low-grade inflammation. The aim of the study was to validate a canine MCP-1 ELISA 
      assay for use in cats and to investigate whether a difference in MCP-1 
      concentrations could be detected between: a) cats having normal or elevated 
      circulating serum amyloid A (SAA) levels and b) normal weight and obese cats. 
      Serum obtained from 36 client-owned cats of various breed, age and sex with 
      normal (n = 20) to elevated SAA (n = 16) was used for the validation of the 
      canine MCP-1 ELISA assay. As no golden standard exists for measurement of 
      inflammation, circulating MCP-1 concentrations were compared to SAA measurements, 
      as an indicator of systemic inflammation. Analytical precision, dilution recovery 
      and detection limit were calculated. A possible correlation between MCP-1 
      concentrations and obesity related measures (body fat percentage (BF%), insulin 
      sensitivity and cytokine expression) were investigated in another population of 
      73 healthy, lean to obese, neutered domestic short-haired cats. RESULTS: Intra- 
      (2.7-4.1%) and inter-assay (2.2-3.6%) coefficient of variation and dilution 
      recovery were acceptable, and the detection limit was 27.1 pg/mL. MCP-1 did not 
      correlate with SAA, and there was no difference between the inflammatory 
      (SAA > 20 mg/L) and non-inflammatory group, due to a marked overlap in MCP-1 
      concentrations. Circulating MCP-1 concentrations were unaffected by BF% 
      (r(2) = 2.7 x 10(-6), P = 0.21) and other obesity-related markers. CONCLUSIONS: 
      The present canine ELISA assay seems to be able to measure circulating feline 
      MCP-1. However, further studies are needed to determine its possible use for 
      detecting inflammation in relation to disease processes or obesity-related 
      low-grade inflammation in cats.
CI  - (c) 2022. The Author(s).
FAU - Stenberg, Kathrine
AU  - Stenberg K
AUID- ORCID: 0000-0003-3275-4256
AD  - Department of Veterinary Clinical Sciences, Faculty of Health and Medical 
      Sciences, University of Copenhagen, Dyrlaegevej 16, 1870, Frederiksberg, Denmark. 
      katst@sund.ku.dk.
FAU - Gensby, Line
AU  - Gensby L
AD  - Department of Veterinary Clinical Sciences, Faculty of Health and Medical 
      Sciences, University of Copenhagen, Dyrlaegevej 16, 1870, Frederiksberg, Denmark.
AD  - AniCura Vangede Animal Hospital, Plantevej 2, 2870, Dyssegard, Denmark.
FAU - Cremer, Signe Emilie
AU  - Cremer SE
AD  - Department of Veterinary Clinical Sciences, Faculty of Health and Medical 
      Sciences, University of Copenhagen, Dyrlaegevej 16, 1870, Frederiksberg, Denmark.
AD  - Coloplast, Holtedam 1-3, 3050, Humlebaek, Denmark.
FAU - Nielsen, Michelle Moller
AU  - Nielsen MM
AD  - Department of Veterinary Clinical Sciences, Faculty of Health and Medical 
      Sciences, University of Copenhagen, Dyrlaegevej 16, 1870, Frederiksberg, Denmark.
FAU - Bjornvad, Charlotte Reinhard
AU  - Bjornvad CR
AD  - Department of Veterinary Clinical Sciences, Faculty of Health and Medical 
      Sciences, University of Copenhagen, Dyrlaegevej 16, 1870, Frederiksberg, Denmark.
LA  - eng
GR  - N2012-0024/Agria Djurforsakring/
PT  - Journal Article
DEP - 20220905
PL  - England
TA  - Acta Vet Scand
JT  - Acta veterinaria Scandinavica
JID - 0370400
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - *Cat Diseases
MH  - Cats
MH  - Chemokine CCL2
MH  - *Dog Diseases
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Humans
MH  - *Inflammation/veterinary
MH  - *Insulin Resistance
MH  - Mice
MH  - Obesity/veterinary
PMC - PMC9446815
OTO - NOTNLM
OT  - Feline
OT  - Inflammation
OT  - MCP-1
OT  - Obesity
OT  - Sensitivity
OT  - Specificity
OT  - Validation
COIS- The authors declare that they have no competing interests.
EDAT- 2022/09/06 06:00
MHDA- 2022/09/08 06:00
PMCR- 2022/09/05
CRDT- 2022/09/05 23:59
PHST- 2021/12/21 00:00 [received]
PHST- 2022/08/22 00:00 [accepted]
PHST- 2022/09/05 23:59 [entrez]
PHST- 2022/09/06 06:00 [pubmed]
PHST- 2022/09/08 06:00 [medline]
PHST- 2022/09/05 00:00 [pmc-release]
AID - 10.1186/s13028-022-00640-3 [pii]
AID - 640 [pii]
AID - 10.1186/s13028-022-00640-3 [doi]
PST - epublish
SO  - Acta Vet Scand. 2022 Sep 5;64(1):22. doi: 10.1186/s13028-022-00640-3.