PMID- 36068768 OWN - NLM STAT- MEDLINE DCOM- 20220908 LR - 20220908 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 241 DP - 2022 Aug TI - Comparative proteomic analysis reveals insights into the response of Cryptolaemus montrouzieri to bottom-up transfer of cadmium and lead across a multi-trophic food chain. PG - 113852 LID - S0147-6513(22)00692-3 [pii] LID - 10.1016/j.ecoenv.2022.113852 [doi] AB - Contamination of agro-ecosystems with heavy metals can affect the development and reproduction of insect natural enemies. This study reports a detailed Tandem Mass Tag based quantitative proteomic analysis of underlying mechanisms responsible for stress response of Cryptolaemus montrouzieri against heavy metals (cadmium (Cd) and lead (Pb)) transported across a multi-trophic food chain. A total of 6639 proteins were detected under Cd as well as Pb stress. In Pb versus the control cluster, 69 proteins (28 up-regulated and 41 down-regulated) were differentially expressed whereas 268 proteins were differentially expressed under Cd versus the control cluster, having 198 proteins up-regulated and 70 down-regulated proteins. The analysis of differentially expressed proteins showed that 27 proteins overlapped in both clusters representing the core proteome to Pb and Cd stress. The bioinformatics analysis demonstrated that these proteins were mapped to 57 and 99 pathways in Pb versus control and Cd versus control clusters, respectively. The functional classification by COG, GO and KEGG databases showed significant changes in protein expression by C. montrouzieri under Pb and Cd stress. The heavy metal stress (Pb and Cd) induced significant changes in expression of proteins like hexokinase (HK), succinyl-CoA, trypsin like proteins, cysteine proteases, cell division cycle proteins, and yellow gene proteins. The results provide detailed information on the protein expression levels of C. montrouzieri and will serve as basic information for future proteomic studies on heavy metal responses of insect predators within a multi-trophic food chain. CI - Copyright (c) 2022. Published by Elsevier Inc. FAU - Wang, Xingmin AU - Wang X AD - Key Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangzhou 510642, PR China; Engineering Research Centre of Agricultural Pest Biocontrol, Ministry of Education and Guangdong Province, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: wangxmcn@scau.edu.cn. FAU - Sang, Wen AU - Sang W AD - Key Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangzhou 510642, PR China; Engineering Research Centre of Agricultural Pest Biocontrol, Ministry of Education and Guangdong Province, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: sangwen@scau.edu.cn. FAU - Xie, Yonghui AU - Xie Y AD - Kunming Branch of Yunnan Provincial Tobacco Company, Kunming 650021, PR China. Electronic address: xiaohui3615@163.com. FAU - Xu, Jing AU - Xu J AD - Key Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangzhou 510642, PR China; Engineering Research Centre of Agricultural Pest Biocontrol, Ministry of Education and Guangdong Province, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: zhibaoxujing@stu.scau.edu.cn. FAU - Sun, Tingfei AU - Sun T AD - Key Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangzhou 510642, PR China; Engineering Research Centre of Agricultural Pest Biocontrol, Ministry of Education and Guangdong Province, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: suntingfei@stu.scau.edu.cn. FAU - Cuthbertson, Andrew G S AU - Cuthbertson AGS AD - Independent Science Advisor, York YO10 5AQ, UK. Electronic address: andrew_cuthbertson@live.co.uk. FAU - Wu, Jianhui AU - Wu J AD - Key Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangzhou 510642, PR China; Engineering Research Centre of Agricultural Pest Biocontrol, Ministry of Education and Guangdong Province, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: jhw@scau.edu.cn. FAU - Ali, Shaukat AU - Ali S AD - Key Laboratory of Bio-Pesticide Innovation and Application, South China Agricultural University, Guangzhou 510642, PR China; Engineering Research Centre of Agricultural Pest Biocontrol, Ministry of Education and Guangdong Province, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: aliscau@scau.edu.cn. LA - eng PT - Journal Article DEP - 20220706 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 RN - 0 (Metals, Heavy) RN - 00BH33GNGH (Cadmium) RN - 2P299V784P (Lead) SB - IM MH - Animals MH - Cadmium/metabolism MH - *Coleoptera/physiology MH - Ecosystem MH - Food Chain MH - Insecta MH - Lead/metabolism/toxicity MH - *Metals, Heavy/metabolism/toxicity MH - Proteomics OTO - NOTNLM OT - Contamination OT - Cryptolaemus montrouzieri OT - Dysmicoccus neobrevipes OT - Solanum melongena OT - TMT technology EDAT- 2022/09/08 06:00 MHDA- 2022/09/09 06:00 CRDT- 2022/09/07 01:46 PHST- 2022/03/21 00:00 [received] PHST- 2022/06/30 00:00 [revised] PHST- 2022/07/02 00:00 [accepted] PHST- 2022/09/07 01:46 [entrez] PHST- 2022/09/08 06:00 [pubmed] PHST- 2022/09/09 06:00 [medline] AID - S0147-6513(22)00692-3 [pii] AID - 10.1016/j.ecoenv.2022.113852 [doi] PST - ppublish SO - Ecotoxicol Environ Saf. 2022 Aug;241:113852. doi: 10.1016/j.ecoenv.2022.113852. Epub 2022 Jul 6.