PMID- 3607230
OWN - NLM
STAT- MEDLINE
DCOM- 19870828
LR  - 20190828
IS  - 0301-4622 (Print)
IS  - 0301-4622 (Linking)
VI  - 26
IP  - 2-3
DP  - 1987 May 9
TI  - Stimulus-secretion coupling mechanisms in mast cells.
PG  - 291-301
AB  - We have investigated several aspects of the complex sequence of events, 
      transmitting the antigen-induced signal of cross-linking immunoglobulin E (IgE) 
      resident in the membrane surface of mast cells into the signals yielding the 
      final process of mediator release. Already the initial phase of this cascade 
      still requires a better understanding. Namely, we are still missing a clear 
      physical description of the effective stimulus-producing antigen-IgE complex in 
      terms of size and spatial requirements. We are investigating this problem on a 
      well-defined cell line (rat basophilic leukemia-RBL-2H3) using synthetic divalent 
      haptens and a monoclonal IgE. A subsequent phase following IgE aggregation is a 
      transient increase in the free calcium concentration in these cells' cytosol. The 
      source of the Ca2+ and the way by which it enters the cytosol are studied 
      predominantly by examining antigen-induced channel activity in the cells' 
      membrane allowing Ca2+ influx from the exterior medium. Finally, we have observed 
      that under certain experimental conditions, antigen-induced degranulation can be 
      achieved even without a rise in cytosolic free calcium. In our search for 
      alternative second messengers, we examine the potential candidacy of the 
      cytosolic Na+/H+ balance. So far, we have found that antigen-stimulated secretion 
      does require extracellular sodium and involves changes in its cytosolic pH. 
      However, further studies are required to clarify its possible role as a coupling 
      element.
FAU - Pecht, I
AU  - Pecht I
FAU - Corcia, A
AU  - Corcia A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, Fc)
RN  - 333DO1RDJY (Serotonin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Basophils
MH  - Calcium/physiology
MH  - Cell Line
MH  - Immunoglobulin E/physiology
MH  - Leukemia, Experimental
MH  - Mast Cells/drug effects/*immunology/metabolism
MH  - Receptors, Antigen, B-Cell/physiology
MH  - Receptors, Fc/physiology
MH  - Serotonin/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1987/05/09 00:00
MHDA- 1987/05/09 00:01
CRDT- 1987/05/09 00:00
PHST- 1987/05/09 00:00 [pubmed]
PHST- 1987/05/09 00:01 [medline]
PHST- 1987/05/09 00:00 [entrez]
AID - 0301-4622(87)80030-3 [pii]
AID - 10.1016/0301-4622(87)80030-3 [doi]
PST - ppublish
SO  - Biophys Chem. 1987 May 9;26(2-3):291-301. doi: 10.1016/0301-4622(87)80030-3.