PMID- 3607277
OWN - NLM
STAT- MEDLINE
DCOM- 19870909
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 70
IP  - 2
DP  - 1987 Aug
TI  - Eosinophils do respond to fMLP.
PG  - 379-83
AB  - Eosinophils were isolated from normal human blood by separation over Percoll 
      gradients, which resulted in eosinophil suspensions of a purity higher than 95% 
      and recoveries of about 65%. Normal human eosinophils were found to respond to 
      formyl-methionyl-leucyl-phenylalanine (fMLP) at concentrations greater than 
      10(-7) mol/L with an increase in the concentration of intracellular free calcium, 
      oxygen consumption, nitroblue tetrazolium reduction, and chemiluminescence. The 
      maximal response of eosinophils to fMLP was lower than that of neutrophils 
      isolated from the same blood samples and required at least ten times as much fMLP 
      as was needed for neutrophils. Low fMLP concentrations (approximately 10(-8) 
      mol/L), which in themselves did not stimulate O2 consumption by either 
      eosinophils or neutrophils, primed these cells to respond to a suboptimal 
      concentration of another stimulus. Purification of eosinophils after treatment of 
      whole blood with fMLP showed that these eosinophils had lost their ability to 
      respond to fMLP. We conclude that normal eosinophils do respond to fMLP and that 
      therefore fMLP should not be used to isolate eosinophils.
FAU - Yazdanbakhsh, M
AU  - Yazdanbakhsh M
FAU - Eckmann, C M
AU  - Eckmann CM
FAU - Koenderman, L
AU  - Koenderman L
FAU - Verhoeven, A J
AU  - Verhoeven AJ
FAU - Roos, D
AU  - Roos D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Acridines)
RN  - 2315-97-1 (10,10'-dimethyl-9,9'-biacridinium)
RN  - 298-83-9 (Nitroblue Tetrazolium)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acridines
MH  - Calcium/metabolism
MH  - Cell Separation/methods
MH  - Cytosol/metabolism
MH  - Eosinophils/*drug effects/metabolism
MH  - Humans
MH  - Luminescent Measurements
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Nitroblue Tetrazolium/metabolism
MH  - Oxidation-Reduction
MH  - Oxygen Consumption/drug effects
EDAT- 1987/08/01 00:00
MHDA- 1987/08/01 00:01
CRDT- 1987/08/01 00:00
PHST- 1987/08/01 00:00 [pubmed]
PHST- 1987/08/01 00:01 [medline]
PHST- 1987/08/01 00:00 [entrez]
AID - S0006-4971(20)78425-9 [pii]
PST - ppublish
SO  - Blood. 1987 Aug;70(2):379-83.