PMID- 36075265 OWN - NLM STAT- Publisher LR - 20231014 IS - 1305-7456 (Print) IS - 1305-7464 (Electronic) VI - 17 IP - 3 DP - 2023 Jul TI - Anti-Peri-implantitis Bacteria's Ability of Robusta Green Coffee Bean (Coffea Canephora) Ethanol Extract: An In Silico and In Vitro Study. PG - 649-662 LID - 10.1055/s-0042-1750803 [doi] AB - OBJECTIVE: This study was aimed to investigate RGCBE extract as antioxidant and anti-peri-implantitis bacteria through in vitro study and its potential as antioxidant, antibacterial, anti-inflammatory, antibone resorption, and proosteogenic through in silico study. MATERIALS: AND METHODS: Absorption, distribution, metabolism, excretion and toxicity prediction, molecular docking simulation, and visualization of chlorogenic acid (CGA) and coumaric acid (CA) as anti-inflammatory, antioxidant, and antibacterial were investigated in silico. Inhibition zone by diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of RGCBE extract against Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), and Prevotella intermedia (Pi) were done. STATISTICAL ANALYSIS: the analysis of variance (ANOVA) difference test, and the post-hoc Tukey's Honest Significant Different (HSD) with a different significance value of p<0.05 RESULTS: GCA and CA compounds are good drug molecules and it has low toxicity. Chlorogenic acid have higher binding activity than coumaric acid to tumor necrosis factor (TNF)-alpha, nuclear factor (NF)-kappaB, receptor activation NF-kappaB (RANK) and its ligand (RANKL), interleukin (IL)-6, IL-10, runt related transcription factor (RUNX2), receptor activator nuclear Kappa beta Ligand-osteoprotegrin osteocalcin (RANKL-OPG), osteocalcin, nuclear factor associated T-cell 1 (NFATc1), tartate resistant acid phosphatase (TRAP), peptidoglycan, flagellin, dectin, Hsp70, and Hsp10 protein. RGCB ethanol extract has high antioxidant ability and it has MIC, MBC, and inhibit the growth of Aa, Pg, Fn, and Pi at 50% concentration with significantly different (p=0.0001 and<0.05). CONCLUSION: RGCB ethanol extract has high antioxidant ability and 50% RGCB ethanol extract may act as strong anti-peri-implantitis bacteria in vitro. In addition, CGA in RGCB potential as antioxidant, antibacterial, anti-inflammatory, antibone resorption, and proosteogenic in silico. CI - The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/). FAU - Nugraha, Alexander Patera AU - Nugraha AP AD - Dental Implant Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. AD - Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. AD - Postgraduate Department of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Ardani, I Gusti Aju Wahju AU - Ardani IGAW AD - Dental Implant Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. AD - Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Sitalaksmi, Ratri Maya AU - Sitalaksmi RM AD - Dental Implant Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. AD - Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Ramadhani, Nastiti Faradilla AU - Ramadhani NF AD - Dental Implant Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. AD - Postgraduate Department of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. AD - Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Rachmayanti, Desi AU - Rachmayanti D AD - Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Kumala, Dina AU - Kumala D AD - Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Kharisma, Viol Dhea AU - Kharisma VD AD - Department of Biology, Faculty of Mathematic and Natural Science, Universitas Brawijaya, Malang, Indonesia. FAU - Rahmadani, Desintya AU - Rahmadani D AD - Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Puspitaningrum, Martining Shoffa AU - Puspitaningrum MS AD - Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Rizqianti, Yuniar AU - Rizqianti Y AD - Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Ari, Muhammad Dimas Aditya AU - Ari MDA AD - Dental Implant Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. AD - Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Nugraha, Albertus Putera AU - Nugraha AP AD - Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Noor, Tengku Natasha Eleena Binti Tengku Ahmad AU - Noor TNEBTA AD - Membership of Faculty of Dental Surgery, Edinburgh University, United Kingdom. AD - Malaysian Armed Forces Dental Officer, 609 Armed Forces Dental Clinic, Kem Semenggo, Kuching, Sarawak, Malaysia. FAU - Luthfi, Muhammad AU - Luthfi M AD - Oral Biology Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia. LA - eng PT - Journal Article DEP - 20220908 PL - Germany TA - Eur J Dent JT - European journal of dentistry JID - 101303672 PMC - PMC10569850 COIS- None declared. EDAT- 2022/09/09 06:00 MHDA- 2022/09/09 06:00 PMCR- 2022/09/01 CRDT- 2022/09/08 19:12 PHST- 2022/09/09 06:00 [pubmed] PHST- 2022/09/09 06:00 [medline] PHST- 2022/09/08 19:12 [entrez] PHST- 2022/09/01 00:00 [pmc-release] AID - 2232044 [pii] AID - 10.1055/s-0042-1750803 [doi] PST - ppublish SO - Eur J Dent. 2023 Jul;17(3):649-662. doi: 10.1055/s-0042-1750803. Epub 2022 Sep 8.