PMID- 36077376 OWN - NLM STAT- MEDLINE DCOM- 20220912 LR - 20220913 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 23 IP - 17 DP - 2022 Sep 1 TI - Decreased Epicardial CTRP3 mRNA Levels in Patients with Type 2 Diabetes Mellitus and Coronary Artery Disease Undergoing Elective Cardiac Surgery: A Possible Association with Coronary Atherosclerosis. LID - 10.3390/ijms23179988 [doi] LID - 9988 AB - (1) Background: C1q TNF-related protein 3 (CTRP3) is an adipokine with anti-inflammatory and cardioprotective properties. In our study, we explored changes in serum CTRP3 and its gene expression in epicardial (EAT) and subcutaneous (SAT) adipose tissue in patients with and without coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM) undergoing elective cardiac surgery. (2) Methods: SAT, EAT, and blood samples were collected at the start and end of surgery from 34 patients: (i) 11 without CAD or T2DM, (ii) 14 with CAD and without T2DM, and (iii) 9 with both CAD and T2DM. mRNA levels of CTRP3 were assessed by quantitative reverse transcription PCR. Circulating levels of CTRP3 and other factors were measured using ELISA and Luminex Multiplex commercial kits. (3) Results: Baseline plasma levels of TNF-alpha and IL6 did not differ among the groups and increased at the end of surgery. Baseline circulating levels of CTRP3 did not differ among the groups and decreased after surgery. In contrast, baseline CTRP3 mRNA levels in EAT were significantly decreased in CAD/T2DM group, while no differences were found for TNF-alpha and IL6 gene expression. (4) Conclusions: Our data suggest that decreased EAT mRNA levels of CTRP3 could contribute to higher risk of atherosclerosis in patients with CAD and T2DM. FAU - Matloch, Zdenek AU - Matloch Z AD - Department of Cardiothoracic Anaesthesia and Intensive Care, The James Cook University Hospital, Marton Road, Middlesbrough TS4 3BW, UK. AD - First Faculty of Medicine, Charles University, Katerinska 32, 121 08 Prague, Czech Republic. FAU - Mraz, Milos AU - Mraz M AD - Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Kasperova, Barbora Judita AU - Kasperova BJ AUID- ORCID: 0000-0003-4578-1093 AD - Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Kratochvilova, Helena AU - Kratochvilova H AD - Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, U Nemocnice 499/2, 128 08 Prague, Czech Republic. AD - Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Svoboda, Petr AU - Svoboda P AUID- ORCID: 0000-0001-7297-4208 AD - Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. AD - Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic. FAU - Pleyerova, Iveta AU - Pleyerova I AUID- ORCID: 0000-0002-6894-1558 AD - Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Reznickova, Katerina AU - Reznickova K AUID- ORCID: 0000-0003-3710-3866 AD - Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Norman, Sarah AU - Norman S AD - Department of Anaesthesia, The James Cook University Hospital, Marton Road, Middlesbrough TS4 3BW, UK. FAU - Hlavacek, Daniel AU - Hlavacek D AUID- ORCID: 0000-0001-8453-1666 AD - Department of Cardiac Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Mahrik, Jakub AU - Mahrik J AUID- ORCID: 0000-0002-9163-1881 AD - Department of Cardiac Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Ivak, Peter AU - Ivak P AD - Department of Cardiac Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. AD - Department of Physiology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic. FAU - Lacinova, Zdenka AU - Lacinova Z AD - Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, U Nemocnice 499/2, 128 08 Prague, Czech Republic. AD - Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Netuka, Ivan AU - Netuka I AD - Department of Cardiac Surgery, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. FAU - Haluzik, Martin AU - Haluzik M AD - Diabetes Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. AD - Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, U Nemocnice 499/2, 128 08 Prague, Czech Republic. LA - eng GR - LX22NPO5104/National Institute for Research of Metabolic and Cardiovascular Diseases/ GR - NV19-02-00118/Institute of Clinical and Experimental Medicine/ PT - Journal Article DEP - 20220901 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Interleukin-6) RN - 0 (RNA, Messenger) RN - 0 (Tumor Necrosis Factor-alpha) SB - IM MH - Adipose Tissue/metabolism MH - *Cardiac Surgical Procedures MH - *Coronary Artery Disease/complications/genetics/surgery MH - *Diabetes Mellitus, Type 2/complications/genetics/surgery MH - Humans MH - Interleukin-6/metabolism MH - Pericardium/metabolism MH - RNA, Messenger/metabolism MH - Tumor Necrosis Factor-alpha/genetics/metabolism PMC - PMC9456433 OTO - NOTNLM OT - CTRP3 OT - adipokines OT - coronary artery disease OT - diabetes mellitus OT - epicardial adipose tissue OT - gene expression COIS- The authors declare no conflict of interest. EDAT- 2022/09/10 06:00 MHDA- 2022/09/14 06:00 PMCR- 2022/09/01 CRDT- 2022/09/09 01:08 PHST- 2022/07/31 00:00 [received] PHST- 2022/08/26 00:00 [revised] PHST- 2022/08/29 00:00 [accepted] PHST- 2022/09/09 01:08 [entrez] PHST- 2022/09/10 06:00 [pubmed] PHST- 2022/09/14 06:00 [medline] PHST- 2022/09/01 00:00 [pmc-release] AID - ijms23179988 [pii] AID - ijms-23-09988 [pii] AID - 10.3390/ijms23179988 [doi] PST - epublish SO - Int J Mol Sci. 2022 Sep 1;23(17):9988. doi: 10.3390/ijms23179988.