PMID- 36077522
OWN - NLM
STAT- MEDLINE
DCOM- 20220912
LR  - 20220913
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 17
DP  - 2022 Sep 4
TI  - Anti-Inflammation and Anti-Pyroptosis Activities of Mangiferin via Suppressing 
      NF-kappaB/NLRP3/GSDMD Signaling Cascades.
LID - 10.3390/ijms231710124 [doi]
LID - 10124
AB  - Mangiferin (MF), a xanthone that extensively exists in many herbal medicines, 
      processes significant activities of anti-inflammation and immunomodulation. The 
      potential regulatory effect and mechanism of mangiferin on cell pyroptosis remain 
      unclear. In this study, mouse bone-marrow-derived macrophages (BMDMs) were 
      stimulated with 1 mug/mL LPS to induce cell pyroptosis and were treated with 10, 
      50, or 100 mug/mL MF for regulating pyroptosis. The cell supernatants TNF-alpha, 
      IL-1beta, IL-6, and IL-18 were detected by enzyme-linked immunosorbent assay 
      (ELISA); gene expression of TNF-alpha, IL-1beta, IL-6, IL-18, Caspase-1, Caspase-11, and 
      gasdermin D (GSDMD) was tested by real-time polymerase chain reaction (RT-PCR), 
      and protein expression levels of apoptosis-associated speck-like protein 
      containing a caspase-recruitment domain (ASC), nod-like receptor protein-3 
      (NLRP3), caspase-1, caspase-11, GSDMD, and NF-kappaB were detected by Western blot. 
      The results showed that MF significantly inhibited the secretion and gene 
      expression of TNF-alpha, IL-6, IL-1beta, and IL-18 that were elevated by LPS. Moreover, 
      MF significantly suppressed the gene expression of Caspase-1, Caspase-11, and 
      GSDMD, and decreased the protein levels of NLRP3, caspase-1, caspase-11, 
      full-length GSDMD (GSDMD-FL), GSDMD N-terminal (GSDMD-N), and NF-kappaB. In 
      conclusion, mangiferin has a multi-target regulating effect on inflammation and 
      pyroptosis by inhibiting the NF-kappaB pathway, suppressing inflammatory 
      caspase-mediated pyroptosis cascades, and reducing GSDMD cleavage in LPS-induced 
      BMDMs.
FAU - Feng, Min
AU  - Feng M
AD  - College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 
      010018, China.
AD  - Engineering Technology Research Center of Traditional Chinese Veterinary Medicine 
      of Gansu Province, Lanzhou Institute of Animal Husbandry and Pharmaceutical 
      Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
FAU - Wei, Shaoqiang
AU  - Wei S
AD  - Engineering Technology Research Center of Traditional Chinese Veterinary Medicine 
      of Gansu Province, Lanzhou Institute of Animal Husbandry and Pharmaceutical 
      Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
FAU - Zhang, Shidong
AU  - Zhang S
AUID- ORCID: 0000-0002-3045-9010
AD  - Engineering Technology Research Center of Traditional Chinese Veterinary Medicine 
      of Gansu Province, Lanzhou Institute of Animal Husbandry and Pharmaceutical 
      Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
FAU - Yang, Ying
AU  - Yang Y
AD  - College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 
      010018, China.
LA  - eng
PT  - Journal Article
DEP - 20220904
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (NLR Proteins)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Xanthones)
RN  - 1M84LD0UMD (mangiferin)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Caspase 1/metabolism
MH  - Caspases
MH  - Inflammasomes/metabolism
MH  - Interleukin-18
MH  - Interleukin-6
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - *NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - NLR Proteins
MH  - Tumor Necrosis Factor-alpha
MH  - *Xanthones/pharmacology
PMC - PMC9456045
OTO - NOTNLM
OT  - GSDMD
OT  - LPS
OT  - inflammatory caspases
OT  - mangiferin
OT  - pyroptosis
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/10 06:00
MHDA- 2022/09/14 06:00
PMCR- 2022/09/04
CRDT- 2022/09/09 01:09
PHST- 2022/08/15 00:00 [received]
PHST- 2022/08/30 00:00 [revised]
PHST- 2022/09/01 00:00 [accepted]
PHST- 2022/09/09 01:09 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2022/09/04 00:00 [pmc-release]
AID - ijms231710124 [pii]
AID - ijms-23-10124 [pii]
AID - 10.3390/ijms231710124 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Sep 4;23(17):10124. doi: 10.3390/ijms231710124.