PMID- 36077946
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220913
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 12
IP  - 17
DP  - 2022 Aug 29
TI  - Histone Methyltransferase SETD2 Is Required for Porcine Early Embryonic 
      Development.
LID - 10.3390/ani12172226 [doi]
LID - 2226
AB  - SET domain-containing 2 (SETD2) is a methyltransferase that can catalyze the di- 
      and tri-methylation of lysine 36 on histone H3 (H3K36me2/me3). SETD2 frequently 
      mediates H3K36me3 modification to regulate both oocyte maturation and 
      preimplantation embryonic development in mice. However, the specific substrate 
      and function of SETD2 in porcine early embryonic development are still unclear. 
      In this study, SETD2 preferentially catalyzed H3K36me3 to regulate porcine early 
      embryonic development. SETD2 mRNA is dynamically expressed during early embryonic 
      development. Functional studies using an RNA interference (RNAi) approach 
      revealed that the expression levels of SETD2 mRNA were effectively knocked down 
      by siRNA microinjection. Immunofluorescence analysis indicated that SETD2 
      knockdown (KD) did not affect H3K36me2 modification but significantly reduced 
      H3K36me3 levels, suggesting a preferential H3K36me3 recognition of SETD2 in 
      porcine embryos. Furthermore, SETD2 KD significantly reduced blastocyst rate and 
      disrupted allocation between inner cell mass (ICM) and trophectoderm (TE) 
      lineage. The expression levels of key genes important for specification of the 
      first two lineages apparently decreased in SETD2 KD blastocysts. SETD2 KD 
      markedly increased the apoptotic percentage of cells within embryos and altered 
      the expression of pro- and anti-apoptotic genes. Therefore, our data indicate 
      that SETD2 is essential for porcine early embryonic development.
FAU - Shao, Weini
AU  - Shao W
AD  - Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource 
      Conservation and Breeding, College of Animal Science and Technology, Anhui 
      Agricultural University, Hefei 230036, China.
FAU - Ning, Wei
AU  - Ning W
AD  - Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource 
      Conservation and Breeding, College of Animal Science and Technology, Anhui 
      Agricultural University, Hefei 230036, China.
FAU - Liu, Chang
AU  - Liu C
AD  - Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource 
      Conservation and Breeding, College of Animal Science and Technology, Anhui 
      Agricultural University, Hefei 230036, China.
FAU - Zou, Yuanyuan
AU  - Zou Y
AD  - Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource 
      Conservation and Breeding, College of Animal Science and Technology, Anhui 
      Agricultural University, Hefei 230036, China.
FAU - Yao, Yurui
AU  - Yao Y
AD  - Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource 
      Conservation and Breeding, College of Animal Science and Technology, Anhui 
      Agricultural University, Hefei 230036, China.
FAU - Kang, Jiaxin
AU  - Kang J
AD  - Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource 
      Conservation and Breeding, College of Animal Science and Technology, Anhui 
      Agricultural University, Hefei 230036, China.
FAU - Cao, Zubing
AU  - Cao Z
AUID- ORCID: 0000-0002-8916-8154
AD  - Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource 
      Conservation and Breeding, College of Animal Science and Technology, Anhui 
      Agricultural University, Hefei 230036, China.
LA  - eng
GR  - 2021YFA0805905/Sub-project of National Key Research and Development Program of 
      China/
GR  - AHCYJSTX-04/Special Fund for Anhui Agriculture Research System/
GR  - 340000211260001000431/Joint Research Project on the Anhui Local Pigs Breeding and 
      Utilization/
GR  - S202110364017/Anhui Provincial Innovation and Entrepreneurship Training Plan for 
      Undergraduate Student/
GR  - 202110364055/Innovation and Entrepreneurship Training Plan of Anhui Agricultural 
      University for Undergraduate Student/
PT  - Journal Article
DEP - 20220829
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC9454584
OTO - NOTNLM
OT  - H3K36me3
OT  - SETD2
OT  - blastocyst
OT  - lineage differentiation
OT  - pig
COIS- The authors declare no conflict of interest with regard to the study.
EDAT- 2022/09/10 06:00
MHDA- 2022/09/10 06:01
PMCR- 2022/08/29
CRDT- 2022/09/09 01:12
PHST- 2022/08/02 00:00 [received]
PHST- 2022/08/22 00:00 [revised]
PHST- 2022/08/24 00:00 [accepted]
PHST- 2022/09/09 01:12 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/10 06:01 [medline]
PHST- 2022/08/29 00:00 [pmc-release]
AID - ani12172226 [pii]
AID - animals-12-02226 [pii]
AID - 10.3390/ani12172226 [doi]
PST - epublish
SO  - Animals (Basel). 2022 Aug 29;12(17):2226. doi: 10.3390/ani12172226.