PMID- 36079819
OWN - NLM
STAT- MEDLINE
DCOM- 20220912
LR  - 20220913
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 14
IP  - 17
DP  - 2022 Aug 29
TI  - Thymus serpyllum Exhibits Anti-Diabetic Potential in Streptozotocin-Induced 
      Diabetes Mellitus Type 2 Mice: A Combined Biochemical and In Vivo Study.
LID - 10.3390/nu14173561 [doi]
LID - 3561
AB  - Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder that is 
      characterized by hyperglycemia, insulin resistance, and lack of insulin 
      production. It has been previously reported that Thymus serpyllum has therapeutic 
      potential against many diseases. To investigate the antidiabetic action of Thymus 
      serpyllum, this study aimed to analyze its restorative impact in diabetic mice, 
      in which it was administered in diet. Diabetes was induced in BALB/c mice fed 
      with a high-fat diet and two intraperitoneal injections of streptozotocin. With 
      the onset of diabetes, the mice were administered daily with aqueous extract of 
      Thymus serpyllum (500 mg/kg/d and 800 mg/kg/d) for 4 weeks. Body weight and 
      fasting blood glucose levels were measured after every 1 week of the treatment. 
      Subsequently, intraperitoneal glucose tolerance and insulin tolerance tests were 
      conducted. In addition, liver tissue was isolated for assessment in terms of 
      levels of gene expression of the AMPK, IRS1, and GLUT2 gene. Treatment with the 
      aqueous extract of Thymus serpyllum was found to be significantly effective in 
      controlling hyperglycemia and improving glucose and insulin tolerance. 
      Predictable with these impacts, the extract of Thymus serpyllum upregulated the 
      AMPK expression at the mRNA level, as well as upregulating the expression of IRS1 
      and GLUT2 gene. Histopathological examination of the liver, kidney, and pancreas 
      also revealed the restorative impact in terms of cellular morphology. The results 
      hence demonstrated that oral administration of aqueous extract of Thymus 
      serpyllum can potentially attenuate hyperglycemia in the liver muscle of 
      streptozotocin (STZ)-induced diabetic mice via AMPK and IRS1 upregulation.
FAU - Azhar, Jahanzaib
AU  - Azhar J
AUID- ORCID: 0000-0002-2210-1422
AD  - Department of Healthcare Biotechnology, Atta-Ur-Rahman School of Applied 
      Biosciences (ASAB), National University of Sciences and Technology (NUST), 
      Islamabad 44000, Pakistan.
FAU - John, Peter
AU  - John P
AD  - Department of Healthcare Biotechnology, Atta-Ur-Rahman School of Applied 
      Biosciences (ASAB), National University of Sciences and Technology (NUST), 
      Islamabad 44000, Pakistan.
FAU - Bhatti, Attya
AU  - Bhatti A
AUID- ORCID: 0000-0002-2781-5221
AD  - Department of Healthcare Biotechnology, Atta-Ur-Rahman School of Applied 
      Biosciences (ASAB), National University of Sciences and Technology (NUST), 
      Islamabad 44000, Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20220829
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - *Diabetes Mellitus, Experimental
MH  - *Diabetes Mellitus, Type 2/drug therapy/metabolism
MH  - Glucose/metabolism
MH  - *Hyperglycemia/drug therapy/metabolism
MH  - Hypoglycemic Agents
MH  - Insulin
MH  - Liver/metabolism
MH  - Mice
MH  - Streptozocin/adverse effects
MH  - *Thymus Plant/metabolism
PMC - PMC9460602
OTO - NOTNLM
OT  - AMPK
OT  - IRS1
OT  - Thymus serpyllum
OT  - glucose and insulin tolerance
OT  - type 2 diabetes
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/10 06:00
MHDA- 2022/09/14 06:00
PMCR- 2022/08/29
CRDT- 2022/09/09 01:25
PHST- 2022/07/19 00:00 [received]
PHST- 2022/08/24 00:00 [revised]
PHST- 2022/08/26 00:00 [accepted]
PHST- 2022/09/09 01:25 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2022/08/29 00:00 [pmc-release]
AID - nu14173561 [pii]
AID - nutrients-14-03561 [pii]
AID - 10.3390/nu14173561 [doi]
PST - epublish
SO  - Nutrients. 2022 Aug 29;14(17):3561. doi: 10.3390/nu14173561.