PMID- 36080128
OWN - NLM
STAT- MEDLINE
DCOM- 20220912
LR  - 20220913
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 17
DP  - 2022 Aug 23
TI  - LC-MS Metabolite Profiling and the Hypoglycemic Activity of Morus alba L. 
      Extracts.
LID - 10.3390/molecules27175360 [doi]
LID - 5360
AB  - Morus alba L. is used in traditional Chinese medicine for its anti-diabetic 
      activity; however, the part of the hypoglycemic activity and related active 
      metabolites are still not fully clarified. In this study, the metabolites in the 
      M. alba roots, leaves, twigs, and fruits extracts (70% ethanol extracts) were 
      systematically identified, and their hypoglycemic activity was evaluated by the 
      high-fat diet/streptozotocin-induced 2 diabetes mellitus (T2D) mouse model. A 
      total of 60 high-level compounds, including 16 polyphenols, 43 flavonoids, and 
      one quinic acid, were identified by high-performance liquid 
      chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) 
      combined with the fragmentation pathways of standards and the self-established 
      database. Among them, 23 metabolites were reported for the first time from this 
      plant. In contrast to the extracts of M. alba leaves and fruits, the extracts of 
      roots and twigs displayed significant hypoglycemic activity The glycemia was 
      significantly reduced from 32.08 +/- 1.27 to 20.88 +/- 1.82 mmol/L and from 33.32 +/- 
      1.98 to 24.74 +/- 1.02 mmol/L, respectively, after 4 weeks of treatment with roots 
      and twigs extracts. Compound 46 (morusin), which is a high-level component 
      identified from the extracts of M. alba roots, also displayed significant 
      activity in decreasing the blood glucose level of T2D mice reduced from 31.45 +/- 
      1.23 to 23.45 +/- 2.13 mmol/L. In addition, the extracts of roots and twigs 
      displayed significant activity in reducing postprandial glycemia. This work marks 
      the first comparison of the metabolites and hypoglycemic activity of M. alba 
      roots, leaves, twigs, and fruits extracts, and provides a foundation for further 
      development of M. alba extracts as anti-diabetic drugs.
FAU - Zhou, Qing Yi-Jun
AU  - Zhou QY
AD  - Science and Technology Innovation Center, Hunan University of Chinese Medicine, 
      Changsha 410208, China.
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 
      410013, China.
FAU - Liao, Xin
AU  - Liao X
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 
      410013, China.
FAU - Kuang, Hao-Ming
AU  - Kuang HM
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 
      410013, China.
FAU - Li, Jia-Yu
AU  - Li JY
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 
      410013, China.
FAU - Zhang, Shui-Han
AU  - Zhang SH
AD  - Science and Technology Innovation Center, Hunan University of Chinese Medicine, 
      Changsha 410208, China.
AD  - Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 
      410013, China.
LA  - eng
GR  - 2020XF6027/The Special Funds for Development of Local Science and Technology from 
      Central Government/
PT  - Journal Article
DEP - 20220823
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Chromatography, Liquid
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - Hypoglycemic Agents/therapeutic use
MH  - Mice
MH  - *Morus/chemistry
MH  - Plant Extracts/chemistry
MH  - Plant Leaves/chemistry
MH  - Tandem Mass Spectrometry
PMC - PMC9457631
OTO - NOTNLM
OT  - LC-MS
OT  - Morus alba L.
OT  - fragmentation pathways
OT  - hypoglycemic activity
OT  - metabolite profiling
OT  - mulberry
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/10 06:00
MHDA- 2022/09/14 06:00
PMCR- 2022/08/23
CRDT- 2022/09/09 01:27
PHST- 2022/07/07 00:00 [received]
PHST- 2022/08/16 00:00 [revised]
PHST- 2022/08/18 00:00 [accepted]
PHST- 2022/09/09 01:27 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2022/08/23 00:00 [pmc-release]
AID - molecules27175360 [pii]
AID - molecules-27-05360 [pii]
AID - 10.3390/molecules27175360 [doi]
PST - epublish
SO  - Molecules. 2022 Aug 23;27(17):5360. doi: 10.3390/molecules27175360.