PMID- 36080161
OWN - NLM
STAT- MEDLINE
DCOM- 20220912
LR  - 20220913
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 17
DP  - 2022 Aug 24
TI  - Effect of Pinoresinol and Vanillic Acid Isolated from Catalpa bignonioides on 
      Mouse Myoblast Proliferation via the Akt/mTOR Signaling Pathway.
LID - 10.3390/molecules27175397 [doi]
LID - 5397
AB  - Growth and maintenance of skeletal muscle is essential for athletic performance 
      and a healthy life. Stimulating the proliferation and differentiation of muscle 
      cells may help prevent loss of muscle mass. To discover effective natural 
      substances enabling to mitigate muscle loss without side effects, we evaluated 
      muscle growth with several compounds extracted from Catalpa bignonioides Walt. 
      Among these compounds, pinoresinol and vanillic acid increased C2C12, a mouse 
      myoblast cell line, proliferation being the most without cytotoxicity. These 
      substances activated the Akt/mammalian target of the rapamycin (mTOR) pathway, 
      which positively regulates the proliferation of muscle cells. In addition, the 
      results of in silico molecular docking study showed that they may bind to the 
      active site of insulin-like growth factor 1 receptor (IGF-1R), which is an 
      upstream of the Akt/mTOR pathway, indicating that both pinoresinol and vanillic 
      acid stimulate myoblast proliferation through direct interaction with IGF-1R. 
      These results suggest that pinoresinol and vanillic acid may be a natural 
      supplement to improve the proliferation of skeletal muscle via IGF-1R/Akt/mTOR 
      signaling and thus strengthen muscles.
FAU - Kim, Seo-Young
AU  - Kim SY
AD  - Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea.
AD  - Honam National Institute of Biological Resources, Mokpo 58762, Korea.
FAU - Kwon, Sung-Pil
AU  - Kwon SP
AD  - Research & Development Center, Chungdam CDC JNPharm LLC., Chuncheon 24341, Korea.
AD  - Department of Biomedical Technology, Kangwon National University, Chuncheon 
      24341, Korea.
FAU - Park, SeonJu
AU  - Park S
AUID- ORCID: 0000-0002-0532-5977
AD  - Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea.
FAU - Cho, Su-Hyeon
AU  - Cho SH
AD  - Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea.
FAU - Oh, Youngse
AU  - Oh Y
AD  - Yonsei Institute of Pharmaceutical Sciences, College of Pharmacy, Yonsei 
      University, Incheon 21983, Korea.
FAU - Kim, Seung Hyun
AU  - Kim SH
AUID- ORCID: 0000-0003-2613-0674
AD  - Yonsei Institute of Pharmaceutical Sciences, College of Pharmacy, Yonsei 
      University, Incheon 21983, Korea.
FAU - Park, Yoon Ho
AU  - Park YH
AD  - Department of Biochemistry, College of Natural Sciences, Kangwon National 
      University, Chuncheon 24341, Korea.
FAU - Jung, Hyun Suk
AU  - Jung HS
AUID- ORCID: 0000-0002-2431-1327
AD  - Department of Biochemistry, College of Natural Sciences, Kangwon National 
      University, Chuncheon 24341, Korea.
FAU - Lee, Deug-Chan
AU  - Lee DC
AD  - Department of Biomedical Technology, Kangwon National University, Chuncheon 
      24341, Korea.
FAU - Jeong, Hoibin
AU  - Jeong H
AUID- ORCID: 0000-0002-0784-5386
AD  - Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea.
AD  - Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 24341, 
      Korea.
FAU - Kim, Kil-Nam
AU  - Kim KN
AUID- ORCID: 0000-0002-7712-7697
AD  - Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea.
LA  - eng
GR  - S3092691/Korea Institute for Advancement of Technology/
PT  - Journal Article
DEP - 20220824
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Furans)
RN  - 0 (Lignans)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - GM8Q3JM2Y8 (Vanillic Acid)
RN  - V4N1UDY811 (pinoresinol)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Furans
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Lignans
MH  - Mammals/metabolism
MH  - Mice
MH  - Molecular Docking Simulation
MH  - Muscle, Skeletal/metabolism
MH  - Myoblasts/metabolism
MH  - *Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - *Vanillic Acid/metabolism/pharmacology
PMC - PMC9457826
OTO - NOTNLM
OT  - Catalpa bignonioides
OT  - IGF-1 signaling
OT  - pinoresinol
OT  - vanillic acid
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/10 06:00
MHDA- 2022/09/14 06:00
PMCR- 2022/08/24
CRDT- 2022/09/09 01:27
PHST- 2022/07/12 00:00 [received]
PHST- 2022/08/19 00:00 [revised]
PHST- 2022/08/20 00:00 [accepted]
PHST- 2022/09/09 01:27 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2022/08/24 00:00 [pmc-release]
AID - molecules27175397 [pii]
AID - molecules-27-05397 [pii]
AID - 10.3390/molecules27175397 [doi]
PST - epublish
SO  - Molecules. 2022 Aug 24;27(17):5397. doi: 10.3390/molecules27175397.