PMID- 36080252
OWN - NLM
STAT- MEDLINE
DCOM- 20220912
LR  - 20220913
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 17
DP  - 2022 Aug 26
TI  - Pro-Apoptotic Effect of Zeolitic Imidazolate Framework-8 (ZIF-8)-Loaded 
      Dihydromyricetin on HepG2 Cells.
LID - 10.3390/molecules27175484 [doi]
LID - 5484
AB  - Dihydromyricetin (DHM) has garnered attention due to its promising antitumor 
      activity, but its low bioavailability restricts its clinical application. Thus, 
      developing nano-drug delivery systems could enhance its antitumor activity. We 
      prepared DHM@ZIF-8 nanoparticles using the zeolite imidazole framework-8 (ZIF-8) 
      as a carrier loaded with dihydromyricetin. A series of characterizations were 
      performed, including morphology, particle size, zeta potential, X-single crystal 
      diffraction, ultraviolet spectroscopy, infrared spectroscopy, and 
      Brunauer-Emmett-Teller (BET). The in vitro release characteristics of DHM@ZIF-8 
      under pH = 5.0 and pH = 7.4 were studied using membrane dialysis. The antitumor 
      activity and pro-apoptotic mechanism of DHM@ZIF-8 were investigated through CCK-8 
      assay, reactive oxygen species (ROS), Annexin V/PI double-staining, transmission 
      electron microscopy, and Western blot. The results depicted that DHM@ZIF-8 
      possessed a regular morphology with a particle size of 211.07 +/- 9.65 nm (PDI: 
      0.19 +/- 0.06) and a Zeta potential of -28.77 +/- 0.67 mV. The 24 h drug releasing 
      rate in PBS solution at pH = 7.4 was 32.08% and at pH = 5.0 was 85.52% in a 
      simulated tumor micro acid environment. DHM@ZIF-8 could significantly enhance the 
      killing effect on HepG2 cells compared to the prodrug. It can effectively remove 
      ROS from the tumor cells, promote apoptosis, and significantly affect the 
      expression of apoptosis-related proteins within tumor cells.
FAU - Mi, Xiao
AU  - Mi X
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing 100193, China.
FAU - Lu, Juan
AU  - Lu J
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing 100193, China.
FAU - Dong, Mingran
AU  - Dong M
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing 100193, China.
FAU - Lou, Yang
AU  - Lou Y
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing 100193, China.
FAU - Zhan, Xia
AU  - Zhan X
AUID- ORCID: 0000-0002-3936-7809
AD  - Key Laboratory of Cleaner Production and Integrated Resource Utilization of China 
      National Light Industry, Beijing Technology and Business University, Beijing 
      100048, China.
FAU - Chen, Xi
AU  - Chen X
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 
      Peking Union Medical College, Beijing 100193, China.
LA  - eng
GR  - No. 2021-I2M-1-022/CAMS Innovation Fund for Medical Science(CIFMS)/
PT  - Journal Article
DEP - 20220826
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Flavonols)
RN  - 0 (Reactive Oxygen Species)
RN  - 1318-02-1 (Zeolites)
RN  - KD8QND6427 (dihydromyricetin)
SB  - IM
MH  - Flavonols
MH  - Hep G2 Cells
MH  - Humans
MH  - Reactive Oxygen Species/metabolism
MH  - Renal Dialysis
MH  - *Zeolites/chemistry/pharmacology
PMC - PMC9458003
OTO - NOTNLM
OT  - HepG2 cells
OT  - ZIF-8
OT  - apoptosis
OT  - dihydromyricetin
OT  - zeolitic imidazolate framework
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/10 06:00
MHDA- 2022/09/14 06:00
PMCR- 2022/08/26
CRDT- 2022/09/09 01:28
PHST- 2022/07/20 00:00 [received]
PHST- 2022/08/22 00:00 [revised]
PHST- 2022/08/23 00:00 [accepted]
PHST- 2022/09/09 01:28 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2022/08/26 00:00 [pmc-release]
AID - molecules27175484 [pii]
AID - molecules-27-05484 [pii]
AID - 10.3390/molecules27175484 [doi]
PST - epublish
SO  - Molecules. 2022 Aug 26;27(17):5484. doi: 10.3390/molecules27175484.