PMID- 36080475
OWN - NLM
STAT- MEDLINE
DCOM- 20220912
LR  - 20240109
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 17
DP  - 2022 Sep 5
TI  - Investigation of Photosystem II Functional Size in Higher Plants under 
      Physiological and Stress Conditions Using Radiation Target Analysis and Sucrose 
      Gradient Ultracentrifugation.
LID - 10.3390/molecules27175708 [doi]
LID - 5708
AB  - The photosystem II (PSII) reaction centre is the critical supramolecular 
      pigment-protein complex in the chloroplast which catalyses the light-induced 
      transfer of electrons from water to plastoquinone. Structural studies have 
      demonstrated the existence of an oligomeric PSII. We carried out radiation 
      inactivation target analysis (RTA), together with sucrose gradient 
      ultracentrifugation (SGU) of PSII, to study the functional size of PSII in 
      diverse plant species under physiological and stress conditions. Two PSII 
      populations, made of dimeric and monomeric core particles, were revealed in Pisum 
      sativum, Spinacea oleracea, Phaseulus vulgaris, Medicago sativa, Zea mais and 
      Triticum durum. However, this core pattern was not ubiquitous in the higher 
      plants since we found one monomeric core population in Vicia faba and a dimeric 
      core in the Triticum durum yellow-green strain, respectively. The PSII functional 
      sizes measured in the plant seedlings in vivo, as a decay of the maximum quantum 
      yield of PSII for primary photochemistry, were in the range of 75-101 +/- 18 kDa, 2 
      to 3 times lower than those determined in vitro. Two abiotic stresses, heat and 
      drought, imposed individually on Pisum sativum, increased the content of the 
      dimeric core in SGU and the minimum functional size determined by RTA in vivo. 
      These data suggest that PSII can also function as a monomer in vivo, while under 
      heat and drought stress conditions, the dimeric PSII structure is predominant.
FAU - Giardi, Maria Teresa
AU  - Giardi MT
AD  - Institute of Crystallography, CNR, Via Salaria Km 29.3, 00016 Monterotondo, 
      Italy.
AD  - Biosensor Srl, Via Olmetti 44, 00060 Formello, Italy.
FAU - Antonacci, Amina
AU  - Antonacci A
AD  - Institute of Crystallography, CNR, Via Salaria Km 29.3, 00016 Monterotondo, 
      Italy.
FAU - Touloupakis, Eleftherios
AU  - Touloupakis E
AUID- ORCID: 0000-0002-5133-0849
AD  - Research Institute on Terrestrial Ecosystems, CNR, Via Madonna del Piano 10, 
      50019 Sesto Fiorentino, Italy.
FAU - Mattoo, Autar K
AU  - Mattoo AK
AD  - USDA-ARS, Sustainable Agricultural Systems Laboratory, Beltsville, MD 20705, USA.
LA  - eng
GR  - 8042-21000-143-00D/United States Department of Agriculture/
PT  - Journal Article
DEP - 20220905
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Photosystem II Protein Complex)
RN  - 57-50-1 (Sucrose)
RN  - OAC30J69CN (Plastoquinone)
SB  - IM
MH  - Pisum sativum
MH  - *Photosystem II Protein Complex/chemistry
MH  - Plastoquinone
MH  - Spinacia oleracea/chemistry
MH  - *Sucrose
MH  - Ultracentrifugation
PMC - PMC9457868
OTO - NOTNLM
OT  - core populations in vitro
OT  - photosystem II
OT  - radiation target analysis
OT  - stress conditions
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/10 06:00
MHDA- 2022/09/14 06:00
PMCR- 2022/09/05
CRDT- 2022/09/09 01:29
PHST- 2022/07/28 00:00 [received]
PHST- 2022/08/31 00:00 [revised]
PHST- 2022/09/01 00:00 [accepted]
PHST- 2022/09/09 01:29 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2022/09/05 00:00 [pmc-release]
AID - molecules27175708 [pii]
AID - molecules-27-05708 [pii]
AID - 10.3390/molecules27175708 [doi]
PST - epublish
SO  - Molecules. 2022 Sep 5;27(17):5708. doi: 10.3390/molecules27175708.