PMID- 36081795
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220912
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - Trichodimerol inhibits inflammation through suppression of the nuclear 
      transcription factor-kappaB/NOD-like receptor thermal protein domain associated 
      protein 3 signaling pathway.
PG  - 999996
LID - 10.3389/fmicb.2022.999996 [doi]
LID - 999996
AB  - Excessive inflammation causes chronic diseases and tissue damage. Although there 
      has been drug treatment, its side effects are relatively large. Searching for 
      effective anti-inflammatory drugs from natural products has become the focus of 
      attention. First isolated from Trichoderma longibraciatum, trichodimerol is a 
      natural product with TNF inhibition. In this study, lipopolysaccharide 
      (LPS)-induced RAW264.7 macrophages were used as a model to investigate the 
      anti-inflammatory activity of trichodimerol. The results of nitric oxide (NO) 
      detection, enzyme-linked immunosorbent assay (ELISA), and reactive oxygen species 
      (ROS) showed that trichodimerol could reduce the production of NO, ROS, and the 
      proinflammatory cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha. 
      Western blotting results showed that trichodimerol could inhibit the production 
      of inflammatory mediators such as cyclooxygenase (COX)-2 and inducible nitric 
      oxide synthase (iNOS) and the protein expression of nuclear transcription 
      factor-kappaB (NF-kappaB), p-IKK, p-IkappaB, Toll-like receptor 4 (TLR4), NOD-like 
      receptor thermal protein domain associated protein 3 (NLRP3), cysteinyl aspartate 
      specific proteinase (Caspase)-1, and ASC, which indicated that trichodimerol may 
      inhibit inflammation through the NF-kappaB and NLRP3 pathways. At the same time, 
      molecular docking showed that trichodimerol can directly combine with the 
      TLR4-MD2 complex. Hence, trichodimerol inhibits inflammation by obstructing the 
      interaction between LPS and the TLR4-MD2 heterodimer and suppressing the 
      downstream NF-kappaB and NLRP3 pathways.
CI  - Copyright (c) 2022 Huo, Lei, Guo, Hu, Kuang, Liu, Peng, Dai, Wang, Gu, Guo and 
      Deng.
FAU - Huo, Xue-Yan
AU  - Huo XY
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Lei, Li-Rong
AU  - Lei LR
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Guo, Wen-Xiu
AU  - Guo WX
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Hu, Yun-Jie
AU  - Hu YJ
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Kuang, Qi-Xuan
AU  - Kuang QX
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Liu, Meng-Dan
AU  - Liu MD
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Peng, Wan
AU  - Peng W
AD  - Institute of Rare Diseases, West China Hospital of Sichuan University, Chengdu, 
      China.
FAU - Dai, Yi-Fei
AU  - Dai YF
AD  - Department of Basic Medical Sciences, School of Medicine, Tsinghua University, 
      Beijing, China.
FAU - Wang, Dong
AU  - Wang D
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Gu, Yu-Cheng
AU  - Gu YC
AD  - Syngenta Jealott's Hill International Research Centre, Berkshire, United Kingdom.
FAU - Guo, Da-Le
AU  - Guo DL
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Deng, Yun
AU  - Deng Y
AD  - State Key Laboratory of Southwestern Chinese Medicine Resources, School of 
      Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20220823
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9445571
OTO - NOTNLM
OT  - NF-kappaB
OT  - NLRP3
OT  - inflammation
OT  - molecular docking
OT  - trichodimerol
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/09/10 06:00
MHDA- 2022/09/10 06:01
PMCR- 2022/08/23
CRDT- 2022/09/09 02:12
PHST- 2022/07/21 00:00 [received]
PHST- 2022/08/01 00:00 [accepted]
PHST- 2022/09/09 02:12 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/10 06:01 [medline]
PHST- 2022/08/23 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.999996 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Aug 23;13:999996. doi: 10.3389/fmicb.2022.999996. 
      eCollection 2022.