PMID- 36081907 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220912 IS - 2296-634X (Print) IS - 2296-634X (Electronic) IS - 2296-634X (Linking) VI - 10 DP - 2022 TI - Novel prognostic matrisome-related gene signature of head and neck squamous cell carcinoma. PG - 884590 LID - 10.3389/fcell.2022.884590 [doi] LID - 884590 AB - Background: Head and neck squamous cell carcinoma (HNSCC) is a common malignancy of the mucosal epithelium of the oral cavity, pharynx, and larynx. Laryngeal squamous cell carcinoma (LSCC) and oral squamous cell carcinoma are common HNSCC subtypes. Patients with metastatic HNSCC have a poor prognosis. Therefore, identifying molecular markers for the development and progression of HNSCC is essential for improving early diagnosis and predicting patient outcomes. Methods: Gene expression RNA-Seq data and patient clinical traits were obtained from The Cancer Genome Atlas-Head and Neck Squamous Cell Carcinoma (TCGA-HNSC) and Gene Expression Omnibus databases. Differentially expressed gene (DEG) screening was performed using the TCGA-HNSC dataset. Intersection analysis between the DEGs and a list of core matrisome genes obtained from the Matrisome Project was used to identify differentially expressed matrisome genes. A prognostic model was established using univariate and multivariate Cox regression analyses, least absolute shrinkage, and selection operator (LASSO) regression analysis. Immune landscape analysis was performed based on the single-sample gene set enrichment analysis algorithm, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, prognostic value, receiver operating characteristic curve analysis, and gene mutation analyses. Immunohistochemical results regarding prognostic protein levels were obtained from the Human Protein Atlas. Single-gene RNA-sequencing data were obtained from GSE150321 and GSE172577 datasets. CCK-8 and Transwell assays were used to confirm cell proliferation and migration. Results: A total of 1,779 DEGs, including 939 upregulated and 840 downregulated genes, between tumor and normal samples were identified using the TCGA-HNSC microarray data. Intersection analysis revealed 52 differentially expressed matrisome-related genes. After performing univariate and multivariate Cox regression and LASSO analyses, a novel prognostic model based on six matrisome genes (FN1, LAMB4, LAMB3, DMP1, CHAD, and MMRN1) for HNSCC was established. This risk model can successfully predict HNSCC survival. The high-risk group had worse prognoses and higher enrichment of pathways related to cancer development than the low-risk group. Silencing LAMB4 in HNSCC cell lines promoted cell proliferation and migration. Conclusion: This study provides a novel prognostic model for HNSCC. Thus, FN1, LAMB4, LAMB3, DMP1, CHAD, and MMRN1 may be the promising biomarkers for clinical practice. CI - Copyright (c) 2022 Huang, Liang, Dong, Huang, Li, Du and Huang. FAU - Huang, Chao AU - Huang C AD - Department of Nephrology, Xiangya Hospital, Central South University, Changsha, China. AD - Department of Otolaryngology-Head and Neck Surgery, Second Xiangya Hospital, Central South University, Changsha, China. FAU - Liang, Yun AU - Liang Y AD - Department of Cell Biology, School of Life Sciences, Central South University, Changsha, China. AD - Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, China. FAU - Dong, Yi AU - Dong Y AD - Department of Cell Biology, School of Life Sciences, Central South University, Changsha, China. AD - Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, China. FAU - Huang, Li AU - Huang L AD - Department of Otolaryngology-Head and Neck Surgery, Second Xiangya Hospital, Central South University, Changsha, China. FAU - Li, Anlei AU - Li A AD - Department of Cell Biology, School of Life Sciences, Central South University, Changsha, China. FAU - Du, Ran AU - Du R AD - Department of Cell Biology, School of Life Sciences, Central South University, Changsha, China. AD - Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, China. FAU - Huang, Hao AU - Huang H AD - Department of Nephrology, Xiangya Hospital, Central South University, Changsha, China. AD - Department of Cell Biology, School of Life Sciences, Central South University, Changsha, China. AD - Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, China. AD - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China. LA - eng PT - Journal Article DEP - 20220823 PL - Switzerland TA - Front Cell Dev Biol JT - Frontiers in cell and developmental biology JID - 101630250 PMC - PMC9445128 OTO - NOTNLM OT - LAMB4 OT - core matrisome gene OT - differentially expressed genes OT - extracellular matrix OT - head and neck squamous cell carcinoma OT - single-cell analysis COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/09/10 06:00 MHDA- 2022/09/10 06:01 PMCR- 2022/01/01 CRDT- 2022/09/09 02:15 PHST- 2022/02/26 00:00 [received] PHST- 2022/07/21 00:00 [accepted] PHST- 2022/09/09 02:15 [entrez] PHST- 2022/09/10 06:00 [pubmed] PHST- 2022/09/10 06:01 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 884590 [pii] AID - 10.3389/fcell.2022.884590 [doi] PST - epublish SO - Front Cell Dev Biol. 2022 Aug 23;10:884590. doi: 10.3389/fcell.2022.884590. eCollection 2022.