PMID- 36082709
OWN - NLM
STAT- MEDLINE
DCOM- 20220912
LR  - 20220912
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 38
IP  - 9
DP  - 2022 Sep
TI  - [Potentilla anserina polysaccharide alleviates the damage of mouse glomerular 
      mesangial cells induced by high glucose via promoting the expression of 
      circ-AKT3].
PG  - 794-800
AB  - Objective To explore the effect of Potentilla anserina polysaccharide on the 
      injury of glomerular mesangial cells induced by high glucose and its possible 
      mechanism. Methods High glucose was used to induce SV40 MES 13 mouse glomerular 
      mesangial cells to establish a cell injury model, and 0.1, 0.3, 0.6 mg/mL 
      Potentilla anserina polysaccharides were added to treat the cells. pcDNA and 
      pcDNA-circ-AKT3 were respectively transfected into mesangial cells induced by 
      high glucose. si-NC and si-circ-AKT3 were transfected into glomerular mesangial 
      cells induced by high glucose and Potentilla anserina polysaccharide was added to 
      the cells. The levels of superoxide dismutase (SOD), glutathione peroxidase 
      (GSH-Px) and malondialedhyde (MDA) were tested according to the kit. The levels 
      of IL-6, IL-18 and monocyte chemoattractant protein-1 (MCP-1) were detected by 
      ELISA. Flow cytometry was used to detect the apoptosis rate. The real time 
      quantitative PCR was used to detect the expression of circ-AKT3 mRNA. Results In 
      glomerular mesangial cells induced by high glucose, the levels of SOD and GSH-Px 
      were decreased, but the levels of MDA, IL-6, IL-18, and MCP-1 were increased, 
      along with the increased rate of apoptosis, and decreased the expression level of 
      circ-AKT3. After Potentilla anserina polysaccharide treatment of glomerular 
      mesangial cells induced by high glucose, the levels of SOD and GSH-Px were 
      increased whereas the levels of MDA, IL-6, IL-18, and MCP-1 were decreased, 
      together with decreased apoptosis rate, and increased expression level of 
      circ-AKT3. The difference was significant among different concentration groups. 
      pcDNA-circ-AKT3 was transfected into glomerular mesangial cells induced by high 
      glucose, followed by the increased levels of SOD and GSH-Px, with the decreased 
      levels of MDA, IL-6, IL-18 and MCP-1. The apoptosis rate was also reported 
      decreased. Interference of circ-AKT3 expression, on the other hand, can restore 
      the protective effect of Potentilla anserina polysaccharide on high glucose 
      induced glomerular mesangial cell injury. Conclusion Potentilla anserina 
      polysaccharide can alleviate the damage of glomerular mesangial cells induced by 
      high glucose by increasing the expression of circ-AKT3.
FAU - Li, Lihua
AU  - Li L
AD  - Department of Nephrology, Qinghai Provincial People's Hospital, Xining 810000, 
      China.
FAU - Wang, Ningning
AU  - Wang N
AD  - Department of Nephrology, Qinghai Provincial People's Hospital, Xining 810000, 
      China.
FAU - Han, Jianfang
AU  - Han J
AD  - Department of Nephrology, Qinghai Provincial People's Hospital, Xining 810000, 
      China.
FAU - Li, Huahua
AU  - Li H
AD  - The First Department of Internal Medicine, Qinghai Kangle Hospital, Xining 
      810000, China.
FAU - Liu, Wenhua
AU  - Liu W
AD  - Department of Nephrology, Qinghai Provincial People's Hospital, Xining 810000, 
      China. *Corresponding author, E-mail: Lwh3653988@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-6)
RN  - 0 (Polysaccharides)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Glucose/metabolism
MH  - Interleukin-18/metabolism
MH  - Interleukin-6/metabolism
MH  - *Mesangial Cells/metabolism
MH  - Mice
MH  - Polysaccharides/pharmacology
MH  - *Potentilla/metabolism
MH  - Superoxide Dismutase/metabolism
EDAT- 2022/09/10 06:00
MHDA- 2022/09/14 06:00
CRDT- 2022/09/09 05:32
PHST- 2022/09/09 05:32 [entrez]
PHST- 2022/09/10 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2022 Sep;38(9):794-800.