PMID- 36087009
OWN - NLM
STAT- MEDLINE
DCOM- 20221201
LR  - 20230211
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 34
IP  - 12
DP  - 2022 Nov 29
TI  - Chromatin-associated microprocessor assembly is regulated by the U1 snRNP 
      auxiliary protein PRP40.
PG  - 4920-4935
LID - 10.1093/plcell/koac278 [doi]
AB  - In plants, microRNA (miRNA) biogenesis involves cotranscriptional processing of 
      RNA polymerase II (RNAPII)-generated primary transcripts by a multi-protein 
      complex termed the microprocessor. Here, we report that Arabidopsis (Arabidopsis 
      thaliana) PRE-MRNA PROCESSING PROTEIN 40 (PRP40), the U1 snRNP auxiliary protein, 
      positively regulates the recruitment of SERRATE, a core component of the plant 
      microprocessor, to miRNA genes. The association of DICER-LIKE1 (DCL1), the 
      microprocessor endoribonuclease, with chromatin was altered in prp40ab mutant 
      plants. Impaired cotranscriptional microprocessor assembly was accompanied by 
      RNAPII accumulation at miRNA genes and retention of miRNA precursors at their 
      transcription sites in the prp40ab mutant plants. We show that cotranscriptional 
      microprocessor assembly, regulated by AtPRP40, positively affects RNAPII 
      transcription of miRNA genes and is important to reach the correct levels of 
      produced miRNAs.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of American 
      Society of Plant Biologists.
FAU - Stepien, Agata
AU  - Stepien A
AUID- ORCID: 0000-0001-9733-7651
AD  - Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology 
      and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
FAU - Dolata, Jakub
AU  - Dolata J
AUID- ORCID: 0000-0001-6141-8863
AD  - Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology 
      and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
FAU - Gulanicz, Tomasz
AU  - Gulanicz T
AUID- ORCID: 0000-0002-8166-4532
AD  - Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology 
      and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
FAU - Bielewicz, Dawid
AU  - Bielewicz D
AUID- ORCID: 0000-0003-0488-7545
AD  - Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology 
      and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
FAU - Bajczyk, Mateusz
AU  - Bajczyk M
AUID- ORCID: 0000-0001-7043-2827
AD  - Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology 
      and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
FAU - Smolinski, Dariusz J
AU  - Smolinski DJ
AUID- ORCID: 0000-0002-8272-0196
AD  - Department of Cellular and Molecular Biology, Nicolaus Copernicus University, 
      Torun 87-100, Poland.
AD  - Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 
      Torun 87-100, Poland.
FAU - Szweykowska-Kulinska, Zofia
AU  - Szweykowska-Kulinska Z
AUID- ORCID: 0000-0003-0032-469X
AD  - Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology 
      and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
FAU - Jarmolowski, Artur
AU  - Jarmolowski A
AUID- ORCID: 0000-0002-1073-146X
AD  - Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology 
      and Biotechnology, Adam Mickiewicz University, Poznan 61-614, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Ribonucleoprotein, U1 Small Nuclear)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - 0 (Chromatin)
SB  - IM
MH  - *Arabidopsis Proteins/genetics/metabolism
MH  - Ribonucleoprotein, U1 Small Nuclear/genetics/metabolism
MH  - *Arabidopsis/genetics/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA Polymerase II/genetics/metabolism
MH  - Microcomputers
MH  - Chromatin/genetics/metabolism
MH  - RNA Processing, Post-Transcriptional/genetics
PMC - PMC9709975
EDAT- 2022/09/11 06:00
MHDA- 2022/12/02 06:00
PMCR- 2022/09/10
CRDT- 2022/09/10 06:23
PHST- 2022/02/28 00:00 [received]
PHST- 2022/09/05 00:00 [accepted]
PHST- 2022/09/11 06:00 [pubmed]
PHST- 2022/12/02 06:00 [medline]
PHST- 2022/09/10 06:23 [entrez]
PHST- 2022/09/10 00:00 [pmc-release]
AID - 6695222 [pii]
AID - koac278 [pii]
AID - 10.1093/plcell/koac278 [doi]
PST - ppublish
SO  - Plant Cell. 2022 Nov 29;34(12):4920-4935. doi: 10.1093/plcell/koac278.