PMID- 36087265
OWN - NLM
STAT- MEDLINE
DCOM- 20220913
LR  - 20220919
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2543
DP  - 2022
TI  - Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics.
PG  - 141-153
LID - 10.1007/978-1-0716-2553-8_12 [doi]
AB  - Ferroptosis is a new form of cell death with hallmark of lipid peroxidation and 
      iron accumulation. It has been shown that lipid peroxidation can result in 
      electrophilic metabolites which in turn induce protein carbonylations. 
      Identification of specific carbonylated proteins and sites in ferroptotic cells 
      will be of great significance for understanding the mechanism and discovering 
      potential biomarkers for this new cell death. The protocol described herein is an 
      optimized pipeline which combines the labeling of carbonylated proteins by a 
      commercially available aniline-based probe with the tandem orthogonal proteolysis 
      activity-based protein profiling (TOP-ABPP) strategy to portrait the landscape of 
      carbonylations in ferroptotic cells.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Chen, Ying
AU  - Chen Y
AD  - Synthetic and Functional Biomolecules Center, Beijing National Laboratory for 
      Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular 
      Engineering of Ministry of Education, College of Chemistry and Molecular 
      Engineering, Peking University, Beijing, China.
FAU - Wang, Chu
AU  - Wang C
AD  - Synthetic and Functional Biomolecules Center, Beijing National Laboratory for 
      Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular 
      Engineering of Ministry of Education, College of Chemistry and Molecular 
      Engineering, Peking University, Beijing, China. chuwang@pku.edu.cn.
AD  - Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China. 
      chuwang@pku.edu.cn.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cell Death
MH  - *Ferroptosis
MH  - Lipid Peroxidation
MH  - Protein Carbonylation
MH  - Proteomics/methods
OTO - NOTNLM
OT  - ABPP
OT  - Aniline-based probe
OT  - DADPS
OT  - Dimethylation
OT  - Ferroptosis
OT  - Protein carbonylations
EDAT- 2022/09/11 06:00
MHDA- 2022/09/14 06:00
CRDT- 2022/09/10 11:20
PHST- 2022/09/10 11:20 [entrez]
PHST- 2022/09/11 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
AID - 10.1007/978-1-0716-2553-8_12 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2543:141-153. doi: 10.1007/978-1-0716-2553-8_12.