PMID- 36087889
OWN - NLM
STAT- MEDLINE
DCOM- 20221011
LR  - 20221027
IS  - 1879-0240 (Electronic)
IS  - 0965-1748 (Linking)
VI  - 149
DP  - 2022 Oct
TI  - TRPV channel nanchung and TRPA channel water witch form insecticide-activated 
      complexes.
PG  - 103835
LID - S0965-1748(22)00117-5 [pii]
LID - 10.1016/j.ibmb.2022.103835 [doi]
AB  - We have previously shown that insect vanilloid-type transient receptor potential 
      (TRPV) channels Nanchung (Nan) and Inactive (Iav) form complexes, which can be 
      over-stimulated and eventually silenced by commercial insecticides, afidopyropen, 
      pymetrozine and pyrifluquinazon. Silencing of the TRPV channels by the 
      insecticides perturbs function of the mechano-sensory organs, chordotonal organs, 
      disrupting sound perception, gravitaxis, and feeding. In addition to TRPV 
      channels, chordotonal organs express an ankyrin-type transient receptor potential 
      (TRPA) channel, Water witch (Wtrw). Genetic data implicate Wtrw in sound and 
      humidity sensing, although the signaling pathway, which links Wtrw to these 
      functions has not been clearly defined. Here we show that, in heterologous 
      system, Nan and Wtrw form calcium channels, which can be activated by 
      afidopyropen, pymetrozine and an endogenous agonist, nicotinamide. Analogous to 
      Nan-Iav heteromers, Nan forms the main binding interface for afidopyropen, 
      whereas co-expression of Wtrw dramatically increases its binding affinity. 
      Pymetrozine competes with afidopyropen for binding to Nan-Wtrw complexes, 
      suggesting that these compounds have overlapping binding sites. Analysis of 
      Drosophila single-nucleus transcriptomic atlas revealed co-expression of nan and 
      wtrw in audio- and mechanosensory neurons. The observation that Nan can form 
      insecticide-sensitive heteromers with more than one type of TRP channels, raises 
      a possibility that Nan may partner with some other TRP channel(s). In addition, 
      we show that Wtrw can be activated by plant-derived reactive electrophiles, allyl 
      isothiocyanate and cinnamaldehyde, defining new molecular target for these 
      repellents.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Kandasamy, Ramani
AU  - Kandasamy R
AD  - BASF Corporation, 26 Davis Drive, Research Triangle Park, NC, 27709, USA.
FAU - Costea, Paul Igor
AU  - Costea PI
AD  - BASF SE, RGD/BE, Carl-Bosch-Strasse 38, 67056, Ludwigshafen am Rhein, Germany.
FAU - Stam, Lynn
AU  - Stam L
AD  - BASF Corporation, 26 Davis Drive, Research Triangle Park, NC, 27709, USA.
FAU - Nesterov, Alexandre
AU  - Nesterov A
AD  - BASF Corporation, 26 Davis Drive, Research Triangle Park, NC, 27709, USA. 
      Electronic address: alexandre.nesterov@basf.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220907
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Ankyrins)
RN  - 0 (Aquaporins)
RN  - 0 (Calcium Channels)
RN  - 0 (Heterocyclic Compounds, 4 or More Rings)
RN  - 0 (Insecticides)
RN  - 0 (Lactones)
RN  - 0 (Transient Receptor Potential Channels)
RN  - 0 (afidopyropen)
RN  - 059QF0KO0R (Water)
RN  - 25X51I8RD4 (Niacinamide)
SB  - IM
MH  - Animals
MH  - Ankyrins/metabolism
MH  - *Aquaporins
MH  - Calcium Channels/genetics
MH  - Drosophila/metabolism
MH  - Heterocyclic Compounds, 4 or More Rings
MH  - *Insecticides/pharmacology
MH  - Lactones
MH  - Niacinamide
MH  - *Transient Receptor Potential Channels/genetics
MH  - Water/metabolism
OTO - NOTNLM
OT  - Insecticides
OT  - Nanchung
OT  - Repellents
OT  - TRPA channels
OT  - TRPV channels
OT  - Water witch
COIS- Declaration of competing interest The authors declare no conflicts of interest 
      associated with this manuscript.
EDAT- 2022/09/11 06:00
MHDA- 2022/10/12 06:00
CRDT- 2022/09/10 19:36
PHST- 2022/05/25 00:00 [received]
PHST- 2022/08/23 00:00 [revised]
PHST- 2022/09/02 00:00 [accepted]
PHST- 2022/09/11 06:00 [pubmed]
PHST- 2022/10/12 06:00 [medline]
PHST- 2022/09/10 19:36 [entrez]
AID - S0965-1748(22)00117-5 [pii]
AID - 10.1016/j.ibmb.2022.103835 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2022 Oct;149:103835. doi: 10.1016/j.ibmb.2022.103835. 
      Epub 2022 Sep 7.