PMID- 36087895 OWN - NLM STAT- MEDLINE DCOM- 20221013 LR - 20221013 IS - 1873-6424 (Electronic) IS - 0269-7491 (Linking) VI - 313 DP - 2022 Nov 15 TI - Associations between internal concentrations of fluorinated and organochlorinated chemicals in women and in vitro fertilization outcomes: A multi-pollutant study. PG - 120087 LID - S0269-7491(22)01301-X [pii] LID - 10.1016/j.envpol.2022.120087 [doi] AB - The impact of persistent organic pollutants (POPs) on reproductive health is still poorly understood, even though infertility management has high associated societal and economical costs. The aims of this study were to characterize the internal levels of polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and perfluoroalkylated substances (PFAS) in women undergoing in vitro fertilization (IVF); and evaluate their association with IVF outcomes, individually and as mixtures in a combined multipollutant approach. Thus, 136 women undergoing IVF treatment at Nantes University Hospital (France) were prospectively recruited between 2019 and 2020. Serum samples were analyzed using liquid chromatography with tandem-mass spectrometry for 14 PFAS. Follicular fluid was analyzed with gas chromatography coupled to high resolution mass spectrometry for 14 PCBs and 25 OCPs. Intermediate and clinical IVF outcomes were ascertained by embryologists and clinicians using standardized protocols. Multivariate Poisson regression models and Bayesian Kernel Machine Regressions (BKMR) were used to identify individual and joint associations between POPs and IVF outcomes adjusting for age, body mass index (BMI) and anti-Mullerian hormone. The results showed that most POPs were widely present in women, and globally not associated with clinically relevant IVF outcomes, like live birth rates. Nonetheless, negative associations between PCB138 and trans-nonachlor with useable blastocysts were identified, beta -0.28 (95%CI [-0.52; -0.04] p = 0.02) and beta -0.22 (95%CI [-0.40; -0.03] p = 0.02). Conversely, PCB28 showed positive associations with the number of useable blastocysts, pregnancy rate and live birth rate. The BKMR analysis suggested the lack of association of the mixture with intermediate and clinical outcomes. The study supports the need of conducting further studies in a larger population sample in order to ensure sufficient statistical power to identify modest effects and a robust stratification analysis to account for the large underlying disease heterogeneity. CI - Copyright (c) 2022 Elsevier Ltd. All rights reserved. FAU - Lefebvre, Tiphaine AU - Lefebvre T AD - LABERCA, Oniris, INRAE, Nantes, France; CHU Nantes, Nantes Universite, Service de Medecine et Biologie de La Reproduction, Gynecologie Medicale, 38 Bd Jean Monnet, Nantes, France; Faculty of Medicine, Nantes Universite, France. FAU - Freour, Thomas AU - Freour T AD - CHU Nantes, Nantes Universite, Service de Medecine et Biologie de La Reproduction, Gynecologie Medicale, 38 Bd Jean Monnet, Nantes, France; Faculty of Medicine, Nantes Universite, France; Nantes Universite, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, F-44000, Nantes, France. FAU - Duval, Gauthier AU - Duval G AD - CHU Nantes, Nantes Universite, Service de Medecine et Biologie de La Reproduction, Gynecologie Medicale, 38 Bd Jean Monnet, Nantes, France. FAU - Ploteau, Stephane AU - Ploteau S AD - CHU Nantes, Nantes Universite, Service de Medecine et Biologie de La Reproduction, Gynecologie Medicale, 38 Bd Jean Monnet, Nantes, France; CHU Nantes, Department of Gynecology and Obstetrics, 38 Bd Jean Monnet, Nantes, France. FAU - Marchand, Philippe AU - Marchand P AD - LABERCA, Oniris, INRAE, Nantes, France. FAU - Le Bizec, Bruno AU - Le Bizec B AD - LABERCA, Oniris, INRAE, Nantes, France. FAU - Antignac, Jean-Philippe AU - Antignac JP AD - LABERCA, Oniris, INRAE, Nantes, France. FAU - Cano-Sancho, German AU - Cano-Sancho G AD - LABERCA, Oniris, INRAE, Nantes, France. Electronic address: german.cano-sancho@oniris-nantes.fr. LA - eng PT - Journal Article DEP - 20220907 PL - England TA - Environ Pollut JT - Environmental pollution (Barking, Essex : 1987) JID - 8804476 RN - 0 (Environmental Pollutants) RN - 0 (Fluorocarbons) RN - 0 (Pesticides) RN - 80497-65-0 (Anti-Mullerian Hormone) RN - DFC2HB4I0K (Polychlorinated Biphenyls) SB - IM MH - Anti-Mullerian Hormone MH - Bayes Theorem MH - *Environmental Pollutants/analysis MH - Female MH - Fertilization in Vitro MH - *Fluorocarbons/analysis MH - Gas Chromatography-Mass Spectrometry MH - Humans MH - Persistent Organic Pollutants MH - *Pesticides/analysis MH - *Polychlorinated Biphenyls/analysis MH - Pregnancy OTO - NOTNLM OT - Follicular fluid OT - In vitro fertilization OT - Infertility OT - Multipollutant OT - Persistent organic pollutants COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2022/09/11 06:00 MHDA- 2022/10/14 06:00 CRDT- 2022/09/10 19:36 PHST- 2022/03/16 00:00 [received] PHST- 2022/08/12 00:00 [revised] PHST- 2022/08/29 00:00 [accepted] PHST- 2022/09/11 06:00 [pubmed] PHST- 2022/10/14 06:00 [medline] PHST- 2022/09/10 19:36 [entrez] AID - S0269-7491(22)01301-X [pii] AID - 10.1016/j.envpol.2022.120087 [doi] PST - ppublish SO - Environ Pollut. 2022 Nov 15;313:120087. doi: 10.1016/j.envpol.2022.120087. Epub 2022 Sep 7.