PMID- 36090682
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220913
IS  - 1226-8453 (Print)
IS  - 2093-4947 (Electronic)
IS  - 1226-8453 (Linking)
VI  - 46
IP  - 5
DP  - 2022 Sep
TI  - Synthesis of ginsenoside Rb(1)-imprinted magnetic polymer nanoparticles for the 
      extraction and cellular delivery of therapeutic ginsenosides.
PG  - 621-627
LID - 10.1016/j.jgr.2022.01.005 [doi]
AB  - BACKGROUND: Panax ginseng (ginseng) is a traditional medicine that is reported to 
      have cardioprotective effects; ginsenosides are the major bioactive compounds in 
      the ginseng root. METHODS: Magnetic molecularly imprinted polymer (MMIP) 
      nanoparticles might be useful for both the extraction of the targeted (imprinted) 
      molecules, and for the delivery of those molecules to cells. In this work, plant 
      growth regulators were used to enhance the adventitious rooting of ginseng root 
      callus; imprinted polymeric particles were synthesized for the extraction of 
      ginsenoside Rb(1) from root extracts, and then employed for subsequent 
      particle-mediated delivery to cardiomyocytes to mitigate hypoxia/reoxygenation 
      injury. RESULTS: These synthesized composite nanoparticles were first 
      characterized by their specific surface area, adsorption capacity, and 
      magnetization, and then used for the extraction of ginsenoside Rb(1) from a crude 
      extract of ginseng roots. The ginsenoside-loaded MMIPs were then shown to have 
      protective effects on mitochondrial membrane potential and cellular viability for 
      H9c2 cells treated with CoCl(2) to mimic hypoxia injury. The protective effect of 
      the ginsenosides was assessed by staining with JC-1 dye to monitor the 
      mitochondrial membrane potential. CONCLUSION: MMIPs can play a dual role in both 
      the extraction and cellular delivery of therapeutic ginsenosides.
CI  - (c) 2022 The Korean Society of Ginseng. Publishing services by Elsevier B.V.
FAU - Liu, Kai-Hsi
AU  - Liu KH
AD  - Department of Internal Medicine, Division of Cardiology, Zuoying Branch of 
      Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
AD  - Department of Electrical Engineering, National University of Kaohsiung, 
      Kaohsiung, Taiwan.
FAU - Lin, Hung-Yin
AU  - Lin HY
AD  - Department of Chemical and Materials Engineering, National University of 
      Kaohsiung, Kaohsiung, Taiwan.
FAU - Thomas, James L
AU  - Thomas JL
AD  - Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, 
      USA.
FAU - Shih, Yuan-Pin
AU  - Shih YP
AD  - Department of Chemical and Materials Engineering, National University of 
      Kaohsiung, Kaohsiung, Taiwan.
FAU - Yang, Zhuan-Yi
AU  - Yang ZY
AD  - Department of Chemical Engineering, I-Shou University, Kaohsiung, Taiwan.
FAU - Chen, Jen-Tsung
AU  - Chen JT
AD  - Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan.
FAU - Lee, Mei-Hwa
AU  - Lee MH
AD  - Department of Materials Science and Engineering, I-Shou University, Kaohsiung, 
      Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20220204
PL  - Korea (South)
TA  - J Ginseng Res
JT  - Journal of ginseng research
JID - 100890690
PMC - PMC9459059
OTO - NOTNLM
OT  - Extraction
OT  - Ginseng root
OT  - Ginsenoside Rb1
OT  - Molecular imprinting
OT  - Myocyte
COIS- All authors declare that they have no conflict of interest.
EDAT- 2022/09/13 06:00
MHDA- 2022/09/13 06:01
PMCR- 2022/02/04
CRDT- 2022/09/12 03:47
PHST- 2021/04/19 00:00 [received]
PHST- 2022/01/14 00:00 [revised]
PHST- 2022/01/26 00:00 [accepted]
PHST- 2022/09/12 03:47 [entrez]
PHST- 2022/09/13 06:00 [pubmed]
PHST- 2022/09/13 06:01 [medline]
PHST- 2022/02/04 00:00 [pmc-release]
AID - S1226-8453(22)00005-7 [pii]
AID - 10.1016/j.jgr.2022.01.005 [doi]
PST - ppublish
SO  - J Ginseng Res. 2022 Sep;46(5):621-627. doi: 10.1016/j.jgr.2022.01.005. Epub 2022 
      Feb 4.