PMID- 36091465
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220913
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 10
DP  - 2022
TI  - 3D-printed collagen/silk fibroin/secretome derived from bFGF-pretreated HUCMSCs 
      scaffolds enhanced therapeutic ability in canines traumatic brain injury model.
PG  - 995099
LID - 10.3389/fbioe.2022.995099 [doi]
LID - 995099
AB  - The regeneration of brain tissue poses a great challenge because of the limited 
      self-regenerative capabilities of neurons after traumatic brain injury (TBI). For 
      this purpose, 3D-printed collagen/silk fibroin/secretome derived from human 
      umbilical cord blood mesenchymal stem cells (HUCMSCs) pretreated with bFGF 
      scaffolds (3D-CS-bFGF-ST) at a low temperature were prepared in this study. From 
      an in vitro perspective, 3D-CS-bFGF-ST showed good biodegradation, appropriate 
      mechanical properties, and good biocompatibility. In regard to vivo, during the 
      tissue remodelling processes of TBI, the regeneration of brain tissues was 
      obviously faster in the 3D-CS-bFGF-ST group than in the other two groups 
      (3D-printed collagen/silk fibroin/secretome derived from human umbilical cord 
      blood mesenchymal stem cells (3D-CS-ST) group and TBI group) by motor assay, 
      histological analysis, and immunofluorescence assay. Satisfactory regeneration 
      was achieved in the two 3D-printed scaffold-based groups at 6 months postsurgery, 
      while the 3D-CS-bFGF-ST group showed a better outcome than the 3D-CS-ST group.
CI  - Copyright (c) 2022 Liu, Zhang, Wei, Hao, Zhong, Zhong, Liu, Liu, Feng, Wang, Zhang, 
      Tian and Zhou.
FAU - Liu, Xiaoyin
AU  - Liu X
AD  - Department of Neurosurgery, West China Hospital, West China Medical School, 
      Sichuan University, Chengdu, China.
FAU - Zhang, Guijun
AU  - Zhang G
AD  - Department of Neurosurgery, West China Hospital, West China Medical School, 
      Sichuan University, Chengdu, China.
FAU - Wei, Pan
AU  - Wei P
AD  - Department of Neurosurgery, The First People's Hospital of Long Quan yi District, 
      Chengdu, China.
FAU - Hao, Lifang
AU  - Hao L
AD  - Department of Radiology, Liao Cheng The Third People's Hospital, Liaocheng, 
      China.
FAU - Zhong, Lin
AU  - Zhong L
AD  - The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
FAU - Zhong, Kunhon
AU  - Zhong K
AD  - State Key Laboratory of Biotherapy, West China Hospital, West China Medical 
      School, Sichuan University, Chengdu, China.
FAU - Liu, Chang
AU  - Liu C
AD  - Department of Neurosurgery, West China Hospital, West China Medical School, 
      Sichuan University, Chengdu, China.
FAU - Liu, Peng
AU  - Liu P
AD  - Department of Neurosurgery, West China Hospital, West China Medical School, 
      Sichuan University, Chengdu, China.
FAU - Feng, Qingbo
AU  - Feng Q
AD  - Department of Liver Surgery and Liver Transplantation, State Key Laboratory of 
      Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, 
      China.
FAU - Wang, Shan
AU  - Wang S
AD  - Department of Neurosurgery, West China Hospital, West China Medical School, 
      Sichuan University, Chengdu, China.
FAU - Zhang, Jianyong
AU  - Zhang J
AD  - Department of General Surgery, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, China.
FAU - Tian, Rui
AU  - Tian R
AD  - Department of Neurosurgery, West China Hospital, West China Medical School, 
      Sichuan University, Chengdu, China.
FAU - Zhou, Liangxue
AU  - Zhou L
AD  - Department of Neurosurgery, West China Hospital, West China Medical School, 
      Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20220824
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC9449499
OTO - NOTNLM
OT  - bFGF
OT  - collagen
OT  - human umbilical cord blood mesenchymal stem cells
OT  - secretome
OT  - silk fibroin
OT  - traumatic brain injury
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/09/13 06:00
MHDA- 2022/09/13 06:01
PMCR- 2022/01/01
CRDT- 2022/09/12 04:00
PHST- 2022/07/15 00:00 [received]
PHST- 2022/08/01 00:00 [accepted]
PHST- 2022/09/12 04:00 [entrez]
PHST- 2022/09/13 06:00 [pubmed]
PHST- 2022/09/13 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 995099 [pii]
AID - 10.3389/fbioe.2022.995099 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2022 Aug 24;10:995099. doi: 10.3389/fbioe.2022.995099. 
      eCollection 2022.